Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,207,066 |
A→G |
E440E (GAA→GAG) |
ymfN → |
predicted DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,207,066 | 0 | A | G | 100.0%
| 75.7
/ NA
| 22 | E440E (GAA→GAG) | ymfN | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (14/8); total (14/8) |
GTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAG > W3110S.gb/1207019‑1207119
|
gTTGCGCTGATTATGGCGGTTGGCAGAGCAATGCTGTACGAGAAAGAGGACACGCTGTCTGATca < 1:411849/65‑1 (MQ=255)
gcgcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACAt < 1:126799/65‑1 (MQ=255)
gcgcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACAt < 1:1556457/65‑1 (MQ=255)
gcgcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACAt < 1:2224114/65‑1 (MQ=255)
gcgcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACAt < 1:345411/65‑1 (MQ=255)
gcgcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACAt < 1:2363316/65‑1 (MQ=255)
cgcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACAt < 1:658933/64‑1 (MQ=255)
ttGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTTATCACAt < 1:2945021/49‑1 (MQ=39)
aCGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGa > 1:2874395/1‑63 (MQ=255)
aCGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg > 1:99017/1‑64 (MQ=255)
aCGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg > 1:1073297/1‑64 (MQ=255)
aCGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg > 1:61841/1‑64 (MQ=255)
aCGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg > 1:469206/1‑64 (MQ=255)
aCGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg > 1:268162/1‑64 (MQ=255)
aCGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg > 1:2636269/1‑64 (MQ=255)
aCGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg > 1:2306938/1‑64 (MQ=255)
aCGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg > 1:2270055/1‑64 (MQ=255)
aCGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg > 1:2030244/1‑64 (MQ=255)
aCGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg > 1:1839951/1‑64 (MQ=255)
aCGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg > 1:1732715/1‑64 (MQ=255)
aCGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg > 1:1608793/1‑64 (MQ=255)
aCGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg > 1:113114/1‑64 (MQ=255)
|
GTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAG > W3110S.gb/1207019‑1207119
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
