Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F17 I0 R2
|
276 |
45.3 |
3729697 |
93.0% |
3468618 |
60.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,207,066 |
A→G |
13.7% |
E440E (GAA→GAG) |
ymfN → |
predicted DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,207,066 | 0 | A | G | 13.7%
| 129.4
/ 13.3
| 51 | E440E (GAA→GAG) | ymfN | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (21/23); new base G (3/4); total (24/27) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GAAAACAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAATT > W3110S.gb/1206998‑1207125
|
gAAAACAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGagaaagaa < 1:1362391/69‑1 (MQ=255)
gAAAACAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGagaaagaa < 1:1596962/69‑1 (MQ=255)
aaaCAAAATCTATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAagagga < 1:890615/69‑1 (MQ=255)
aaaCAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAagagga < 1:2935727/69‑1 (MQ=255)
aaaCAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAagagga > 1:2567979/1‑69 (MQ=255)
aaaCAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAagagga > 1:255458/1‑69 (MQ=255)
aaaCAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAagagga < 1:1508886/69‑1 (MQ=255)
aaaCAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAagagga < 1:2009819/69‑1 (MQ=255)
aaaCAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAagaaga > 1:854344/1‑69 (MQ=255)
aaaCAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAagaaga < 1:1768168/69‑1 (MQ=255)
aaCAAAATCGCTGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGac > 1:1811839/1‑69 (MQ=255)
aaCAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGac > 1:921168/1‑69 (MQ=255)
aaCAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGac > 1:2527164/1‑69 (MQ=255)
aaCAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGac > 1:1268613/1‑69 (MQ=255)
cAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGacac < 1:969468/69‑1 (MQ=255)
cAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGacac < 1:2302733/69‑1 (MQ=255)
aTCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTg < 1:1073331/69‑1 (MQ=255)
tCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGaca > 1:3416288/1‑63 (MQ=255)
ggTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGacac > 1:1705844/1‑59 (MQ=255)
ggTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTg > 1:873829/1‑63 (MQ=255)
ggTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGAt > 1:2257140/1‑69 (MQ=255)
ggTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGAt < 1:2300323/69‑1 (MQ=255)
gCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATcac < 1:1090329/69‑1 (MQ=255)
gCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATcac < 1:1725077/69‑1 (MQ=255)
gCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATcac < 1:3622588/69‑1 (MQ=255)
gCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATca < 1:930801/68‑1 (MQ=255)
gCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATca < 1:854947/68‑1 (MQ=255)
ttGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTg > 1:2955615/1‑69 (MQ=255)
gcgcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAg < 1:2064427/69‑1 (MQ=255)
gcgcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAg > 1:3377311/1‑69 (MQ=255)
gcgcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAg > 1:2322285/1‑69 (MQ=255)
gcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGt < 1:1661574/68‑1 (MQ=255)
gcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGt < 1:1198254/68‑1 (MQ=255)
gcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGt < 1:1202550/68‑1 (MQ=255)
gcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGt < 1:2518936/68‑1 (MQ=255)
gcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGt < 1:13224/68‑1 (MQ=255)
cTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTcc < 1:1250534/69‑1 (MQ=255)
cTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTcc < 1:2628795/69‑1 (MQ=255)
cTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTcc < 1:295130/69‑1 (MQ=255)
gATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTa < 1:3061684/69‑1 (MQ=255)
aTTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAagaaga > 1:2743107/1‑41 (MQ=255)
aTTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAagaaga < 1:240032/41‑1 (MQ=255)
aTTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAagaaga < 1:2137621/41‑1 (MQ=255)
aTTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAagaaga < 1:1181153/41‑1 (MQ=255)
aTTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTaa > 1:283312/1‑68 (MQ=255)
ggTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACg < 1:369372/62‑1 (MQ=255)
ggTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACg < 1:1636501/62‑1 (MQ=255)
ttGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATca < 1:1997261/46‑1 (MQ=255)
gagCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCttt < 1:412626/69‑1 (MQ=255)
agCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTa < 1:1115460/69‑1 (MQ=255)
tGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGa < 1:2991795/69‑1 (MQ=255)
tGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGa < 1:960623/69‑1 (MQ=255)
cTGTACGAGAAAGAGGACACTCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg > 1:240350/1‑68 (MQ=255)
cTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg > 1:2909583/1‑68 (MQ=255)
cTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg > 1:1590065/1‑68 (MQ=255)
cTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg > 1:3698546/1‑68 (MQ=255)
tGTACGCGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCttt > 1:478353/1‑60 (MQ=255)
tGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCttt > 1:2852286/1‑60 (MQ=255)
tGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCttt > 1:1139987/1‑60 (MQ=255)
gTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGt < 1:1641663/38‑1 (MQ=255)
cGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAAtt > 1:70470/1‑69 (MQ=255)
cGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAAtt > 1:395315/1‑69 (MQ=255)
cGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAAtt > 1:928259/1‑69 (MQ=255)
cGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAAtt > 1:1905394/1‑69 (MQ=255)
cGAGAAAGAAGACACGCTGACTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAAt > 1:2583461/1‑68 (MQ=255)
agaagaCACGCTGTCTGATCACATTGAGTCCTACGGGATCcgctcgc < 1:281295/47‑1 (MQ=255)
agaagaCACGCTGTCTGATCACATTGAGTCCTACGGGATCcgctcgc < 1:2860077/47‑1 (MQ=255)
|
GAAAACAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAATT > W3110S.gb/1206998‑1207125
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
