Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,012,196 |
C→T |
T80I (ACT→ATT) |
yedK → |
hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,012,196 | 0 | C | T | 100.0%
| 11.5
/ NA
| 5 | T80I (ACT→ATT) | yedK | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (5/0); total (5/0) |
CCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCAACCAGTCGTAT > W3110S.gb/2012150‑2012214
|
cccGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAATTGCGGCAACCAGTCGTAt > 1:2284314/1‑65 (MQ=255)
cccGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAATTGCGGCAACCAGTCGTAt > 1:2287924/1‑65 (MQ=255)
cccGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAATTGCGGCAACCAGTCGTAt > 1:2453991/1‑65 (MQ=255)
cccGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAATTGCGGCAACCAGTCGTAt > 1:2722093/1‑65 (MQ=255)
cccGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAATTGCGGCAACCAGTCGTAt > 1:2842557/1‑65 (MQ=255)
|
CCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCAACCAGTCGTAT > W3110S.gb/2012150‑2012214
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
