Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,215,186 |
A→G |
V115V (GTT→GTC) |
yehS ← |
conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,215,186 | 0 | A | G | 100.0%
| 56.4
/ NA
| 16 | V115V (GTT→GTC) | yehS | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (0/16); total (0/16) |
TTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGG > W3110S.gb/2215141‑2215205
|
tttATGATCCGGTGCGCGCATCATCGCTTTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg < 1:3064022/65‑1 (MQ=255)
tttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg < 1:1040518/65‑1 (MQ=255)
tttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg < 1:1051204/65‑1 (MQ=255)
tttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg < 1:1175513/65‑1 (MQ=255)
tttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg < 1:147561/65‑1 (MQ=255)
tttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg < 1:2397775/65‑1 (MQ=255)
tttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg < 1:2503212/65‑1 (MQ=255)
tttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg < 1:253201/65‑1 (MQ=255)
tttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg < 1:2958127/65‑1 (MQ=255)
tttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg < 1:45205/65‑1 (MQ=255)
tttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg < 1:580067/65‑1 (MQ=255)
tttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg < 1:792773/65‑1 (MQ=255)
tttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg < 1:89911/65‑1 (MQ=255)
tttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg < 1:993537/65‑1 (MQ=255)
tttATGATCCGGAGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg < 1:1262209/65‑1 (MQ=255)
ttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCgg < 1:1325761/64‑1 (MQ=255)
|
TTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGG > W3110S.gb/2215141‑2215205
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
