Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,215,186 |
A→G |
V115V (GTT→GTC) |
yehS ← |
conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,215,186 | 0 | A | G | 100.0%
| 162.7
/ NA
| 47 | V115V (GTT→GTC) | yehS | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (16/31); total (16/31) |
GCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAACGC > W3110S.gb/2215123‑2215245
|
gccgcATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCTTCGCTGTAATTTCCGGCATCGAGACGCgg < 1:2158736/70‑1 (MQ=255)
gccgcATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCgg < 1:709359/70‑1 (MQ=255)
gccgcATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCgg < 1:339732/70‑1 (MQ=255)
gccgcATTCGCGGAAGTTTTTATGATCCGGTGCCCGCATCATCGCTGTAATTTCCGGCATCGAGACGCgg < 1:4247131/70‑1 (MQ=255)
cATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAActg < 1:2246411/71‑1 (MQ=255)
ttCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAActgct > 1:3902365/1‑71 (MQ=255)
ttCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAActgct > 1:3688787/1‑71 (MQ=255)
cgcgGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGt > 1:3157117/1‑71 (MQ=255)
cgcgGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGt > 1:2756701/1‑71 (MQ=255)
gcgGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGtt < 1:4094185/71‑1 (MQ=255)
tttttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAg < 1:2439224/71‑1 (MQ=255)
ttttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAg > 1:1029429/1‑70 (MQ=255)
ttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGATGCGGAAGTGCTGTTCGGTGAGGa < 1:3171736/70‑1 (MQ=255)
ttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGa < 1:772874/70‑1 (MQ=255)
aTGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATa < 1:4209337/70‑1 (MQ=255)
tCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAg < 1:3756962/71‑1 (MQ=255)
tCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAg < 1:3670485/71‑1 (MQ=255)
tCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAg < 1:938909/71‑1 (MQ=255)
tCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAg < 1:2984815/71‑1 (MQ=255)
tCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAg < 1:3009955/71‑1 (MQ=255)
tCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAg < 1:2142744/71‑1 (MQ=255)
atcatcGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGt > 1:4130080/1‑71 (MQ=255)
catcGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGttt > 1:945430/1‑71 (MQ=255)
atcGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCATaa > 1:713320/1‑58 (MQ=255)
atcGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGaa > 1:3224148/1‑58 (MQ=255)
atcGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGaa > 1:2253374/1‑58 (MQ=255)
atcGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGtttt > 1:2717586/1‑71 (MQ=255)
atcGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGtttt > 1:2496609/1‑71 (MQ=255)
atcGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGtttt > 1:3899336/1‑71 (MQ=255)
atcGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGtttt > 1:152700/1‑71 (MQ=255)
tAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGt < 1:1589268/54‑1 (MQ=255)
tAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGaa < 1:2239151/71‑1 (MQ=255)
tAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGaa < 1:1729736/71‑1 (MQ=255)
tAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGaa < 1:758026/71‑1 (MQ=255)
tAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGaa < 1:1092063/71‑1 (MQ=255)
tCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgc > 1:3711038/1‑71 (MQ=255)
tCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgc > 1:60648/1‑71 (MQ=255)
ccGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgc < 1:898011/70‑1 (MQ=255)
ccGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgc < 1:3316651/70‑1 (MQ=255)
ccGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgc < 1:626524/70‑1 (MQ=255)
ccGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgc < 1:4573351/70‑1 (MQ=255)
ccGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgc < 1:4511912/70‑1 (MQ=255)
ccGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgc < 1:2717468/70‑1 (MQ=255)
ccGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgc < 1:1962617/70‑1 (MQ=255)
ccGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgc < 1:1395916/70‑1 (MQ=255)
ccGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgc < 1:1038344/70‑1 (MQ=255)
ccGGCAGCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgc < 1:4101728/70‑1 (MQ=255)
|
GCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAACGC > W3110S.gb/2215123‑2215245
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
