Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,257,455 |
C→T |
G9R (GGA→AGA) |
yeiJ ← |
predicted nucleoside transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,257,455 | 0 | C | T | 98.6%
| 218.5
/ ‑4.2
| 69 | G9R (GGA→AGA) | yeiJ | predicted nucleoside transporter |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (0/68); total (1/68) |
| Fisher's exact test for biased strand distribution p-value = 1.45e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 |
TTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCCCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACGGC > W3110S.gb/2257403‑2257514
|
ttcttcttGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTtc < 1:49125/64‑1 (MQ=255)
tcttGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTtc < 1:1464960/60‑1 (MQ=255)
tcttGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTtc < 1:224852/60‑1 (MQ=255)
tcttGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTtc < 1:2344943/60‑1 (MQ=255)
tcttGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTtc < 1:2395591/60‑1 (MQ=255)
tcttGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTtc < 1:2551055/60‑1 (MQ=255)
tcttGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTtc < 1:2763428/60‑1 (MQ=255)
tcttGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTtc < 1:291351/60‑1 (MQ=255)
tcttGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTtc < 1:16305/60‑1 (MQ=255)
tcttGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTtc < 1:162203/60‑1 (MQ=255)
tcttGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTtc < 1:1538027/60‑1 (MQ=255)
tcttGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTtc < 1:1402361/60‑1 (MQ=255)
tcttGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTtc < 1:1376666/60‑1 (MQ=255)
tcttGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTtc < 1:493731/60‑1 (MQ=255)
tcttGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTtc < 1:590223/60‑1 (MQ=255)
tcttGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTtc < 1:1276623/60‑1 (MQ=255)
tcttGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTtc < 1:729952/60‑1 (MQ=255)
tcttGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTtc < 1:842639/60‑1 (MQ=255)
tcttGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTtc < 1:933494/60‑1 (MQ=255)
tcttGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTtc < 1:985324/60‑1 (MQ=255)
cttGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTtc < 1:5539/59‑1 (MQ=255)
cttGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTtc < 1:2056543/59‑1 (MQ=255)
tGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGACATCCATa < 1:2944945/64‑1 (MQ=255)
tGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGACATCCATa < 1:2538759/64‑1 (MQ=255)
tGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGACATCCATa < 1:2825431/64‑1 (MQ=255)
tGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGACATCCATa < 1:226778/64‑1 (MQ=255)
tGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGACATCCATa < 1:2036751/64‑1 (MQ=255)
tGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGACATCCATa < 1:1429955/64‑1 (MQ=255)
tGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGACATCCATa < 1:1305227/64‑1 (MQ=255)
tGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGACATCCATa < 1:1287370/64‑1 (MQ=255)
gataGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAg < 1:3014525/61‑1 (MQ=255)
gACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGACATCCATa < 1:530101/63‑1 (MQ=255)
gACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAg < 1:2670373/64‑1 (MQ=255)
gACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAg < 1:450924/64‑1 (MQ=255)
gACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAg < 1:1973451/64‑1 (MQ=255)
gACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAg < 1:2579335/64‑1 (MQ=255)
gACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAg < 1:1222419/64‑1 (MQ=255)
gACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAg < 1:2542508/64‑1 (MQ=255)
gACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAg < 1:119180/64‑1 (MQ=255)
gACAGTAAAAACGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAg < 1:243742/64‑1 (MQ=255)
aaaaaCGCAATCGTCAGCAATACCACCATTCTCAGAACACTtc < 1:403592/43‑1 (MQ=255)
cGCAATCGTCAGCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAg < 1:1678376/53‑1 (MQ=255)
ttCAGCAATACCACCATTCTCAGAACACTTCTCATGa < 1:774390/36‑1 (MQ=37)
gTCAGCAATACCACCATTCTCAGAACACTTCTCATGa < 1:2596673/37‑1 (MQ=255)
gTCAGCAATACCACCATTCTCAGAACACTTCTCATGa < 1:2435148/37‑1 (MQ=255)
aGCAATACCACCATTCTCAGAACACTTCTCATGACATCCATAg < 1:879010/43‑1 (MQ=255)
ccaccaTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATg < 1:1060143/52‑1 (MQ=255)
accaTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATTTCATATTGGGCGACg < 1:1348744/65‑1 (MQ=255)
accaTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACg < 1:511827/65‑1 (MQ=255)
accaTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACg < 1:1211230/65‑1 (MQ=255)
accaTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACg < 1:1317075/65‑1 (MQ=255)
accaTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACg < 1:2985366/65‑1 (MQ=255)
accaTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACg < 1:480111/65‑1 (MQ=255)
accaTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACg < 1:704252/65‑1 (MQ=255)
accaTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACg < 1:476353/65‑1 (MQ=255)
accaTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACg < 1:434633/65‑1 (MQ=255)
accaTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACg < 1:405944/65‑1 (MQ=255)
accaTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACg < 1:361020/65‑1 (MQ=255)
accaTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACg < 1:2150336/65‑1 (MQ=255)
accaTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACg < 1:2910763/65‑1 (MQ=255)
accaTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACg < 1:2867114/65‑1 (MQ=255)
accaTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACg < 1:1750070/65‑1 (MQ=255)
accaTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACg < 1:2545170/65‑1 (MQ=255)
accaTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACg < 1:1008006/65‑1 (MQ=255)
accaTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACg < 1:2523685/65‑1 (MQ=255)
accaTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACg < 1:2412832/65‑1 (MQ=255)
accaTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACg < 1:2022042/65‑1 (MQ=255)
accaTTCTCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACg < 1:228506/65‑1 (MQ=255)
caTTCCCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACGGc > 1:2019762/1‑65 (MQ=255)
|
TTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCCCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACGGC > W3110S.gb/2257403‑2257514
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
