Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F17 I0 R2
|
278 |
43.2 |
2215644 |
94.6% |
2095999 |
61.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,833,531 |
C→T |
41.3% |
R92C (CGC→TGC) |
ygbL → |
predicted class II aldolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,833,531 | 0 | C | T | 41.3%
| 28.5
/ 51.7
| 46 | R92C (CGC→TGC) | ygbL | predicted class II aldolase |
| Reads supporting (aligned to +/- strand): ref base C (14/13); new base T (11/8); total (25/21) |
| Fisher's exact test for biased strand distribution p-value = 7.69e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGA > minE/1833463‑1833598
|
aaTGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCgtgc < 1:2143878/71‑1 (MQ=255)
gTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTa < 1:1469001/71‑1 (MQ=255)
aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCggtgg > 1:1999314/1‑69 (MQ=255)
aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCggtgg > 1:1544047/1‑69 (MQ=255)
aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCggtgg > 1:484380/1‑69 (MQ=255)
aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCggtgg > 1:486984/1‑69 (MQ=255)
aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCggtg > 1:412958/1‑68 (MQ=255)
aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCggtg > 1:571993/1‑68 (MQ=255)
cAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGCTGGTGc < 1:738210/71‑1 (MQ=255)
aaaCCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCa > 1:926848/1‑71 (MQ=255)
aCCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCAtt > 1:524186/1‑71 (MQ=255)
ccTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTg < 1:1026852/71‑1 (MQ=255)
ccTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTg < 1:441724/71‑1 (MQ=255)
tCGAAAGATGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGc < 1:110350/64‑1 (MQ=255)
gAAAGAGGTGCTCTTTCATCTGGCTCTGTATCGCAACAATCCGTGCTGTAAAGCggtgg > 1:1794293/1‑59 (MQ=255)
gAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTgca < 1:139737/69‑1 (MQ=255)
gAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTgca > 1:1139802/1‑69 (MQ=255)
tGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATgg < 1:1070975/71‑1 (MQ=255)
ctTTCATCTGGCGCTGTATCGCAACAATCCTCGCTGTAAAGCGGTGGTGCATTTgcaca < 1:1204445/59‑1 (MQ=255)
ctTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGa > 1:243732/1‑71 (MQ=255)
tttCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCggt < 1:682128/44‑1 (MQ=255)
tttCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCggt < 1:1855314/44‑1 (MQ=255)
tCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTgcacagcaca > 1:94713/1‑61 (MQ=255)
tCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTgcacagcaca > 1:639439/1‑61 (MQ=255)
tCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTgcacagcaca > 1:2182581/1‑61 (MQ=255)
tCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCggtg > 1:2161693/1‑43 (MQ=255)
tCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACgg > 1:171382/1‑71 (MQ=255)
ggCGCTGTATCGCAACAATCCGCGCTGTAAAGCggtggt > 1:1834796/1‑39 (MQ=255)
gcgcTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCtt < 1:1278915/69‑1 (MQ=255)
tGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCttt < 1:2116278/66‑1 (MQ=255)
tATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCttt > 1:550471/1‑64 (MQ=255)
tCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCctgcctgc < 1:1474944/71‑1 (MQ=255)
tCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCctgcctgc < 1:1088157/71‑1 (MQ=255)
tCGCAACAATCCGCGCTGTAAAGCGGTTGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCctgcctgc < 1:1206888/71‑1 (MQ=255)
tCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTcctgcctg > 1:1481293/1‑70 (MQ=255)
tCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTcctgcctg > 1:881752/1‑70 (MQ=255)
tCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTcctg < 1:1603360/66‑1 (MQ=255)
tCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCctgcctgc < 1:302785/71‑1 (MQ=255)
tCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCctgcctgc < 1:1731938/71‑1 (MQ=255)
tCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCctgcctgc < 1:1248207/71‑1 (MQ=255)
gCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCctgcctgc < 1:503158/69‑1 (MQ=255)
gCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCctgcctgc > 1:1030593/1‑69 (MQ=255)
gCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCctgcctgc > 1:972241/1‑69 (MQ=255)
gCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCctgcctgc > 1:125385/1‑69 (MQ=255)
gaacaaTCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAg > 1:940765/2‑71 (MQ=255)
acaaTCCTCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAggg < 1:136197/71‑1 (MQ=255)
aaTCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCt > 1:245978/1‑71 (MQ=255)
cgcgCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGa > 1:978695/1‑70 (MQ=255)
|
AATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGA > minE/1833463‑1833598
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
