Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F28 I3 R1
|
176 |
0.0 |
1562086 |
58.2% |
909134 |
66.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
2,955,451 |
2 bp→CT |
coding (516‑517/678 nt) |
yjjG → |
predicted hydrolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,955,451 | 0 | G | C | 100.0%
| 41.1
/ NA
| 13 | L172L (CTG→CTC) | yjjG | predicted hydrolase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (0/13); total (0/13) |
| * | minE | 2,955,452 | 0 | A | T | 100.0%
| 42.1
/ NA
| 13 | M173L (ATG→TTG) | yjjG | predicted hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (0/13); total (0/13) |
TTCACGCGTGCTGATGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCG > minE/2955439‑2955508
||
cagaCGTGTGCTCTTGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCg < 1:1010532/67‑1 (MQ=255)
cagaCGTGTGCTCTTGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCg < 1:1081837/67‑1 (MQ=255)
cagaCGTGTGCTCTTGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCg < 1:1148523/67‑1 (MQ=255)
cagaCGTGTGCTCTTGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCg < 1:1169991/67‑1 (MQ=255)
cagaCGTGTGCTCTTGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCg < 1:126170/67‑1 (MQ=255)
cagaCGTGTGCTCTTGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCg < 1:1482920/67‑1 (MQ=255)
cagaCGTGTGCTCTTGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCg < 1:1485783/67‑1 (MQ=255)
cagaCGTGTGCTCTTGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCg < 1:256844/67‑1 (MQ=255)
cagaCGTGTGCTCTTGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCg < 1:31298/67‑1 (MQ=255)
cagaCGTGTGCTCTTGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCg < 1:411690/67‑1 (MQ=255)
cagaCGTGTGCTCTTGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCg < 1:717887/67‑1 (MQ=255)
cagaCGTGTGCTCTTGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCg < 1:913216/67‑1 (MQ=255)
cagaCGTGTGCTCTTGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCg < 1:997480/67‑1 (MQ=255)
||
TTCACGCGTGCTGATGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCG > minE/2955439‑2955508
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
