Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I1 R2 146 74.7 3299319 84.8% 2797822 66.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 575,416 T→C N135S (AAT→AGT)  ybjX ← conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE575,4160TC100.0% 383.2 / NA 101N135S (AAT→AGT) ybjXconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (69/32);  total (69/32)

Only 100 of 101 total aligned reads displayed.

CTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTATTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAT  >  minE/575367‑575467
                                                 |                                                   
cTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAATTATAAGCTGAAttctt                                >  1:1541959/1‑71 (MQ=255)
cTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttctt                                >  1:250034/1‑71 (MQ=255)
cTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttctt                                >  1:702529/1‑71 (MQ=255)
cTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttctt                                >  1:563103/1‑71 (MQ=255)
cTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttctt                                >  1:2967795/1‑71 (MQ=255)
cTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttctt                                >  1:290773/1‑71 (MQ=255)
cTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttctt                                >  1:2569697/1‑71 (MQ=255)
cTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttctt                                >  1:1876325/1‑71 (MQ=255)
cTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttctt                                >  1:1659733/1‑71 (MQ=255)
cTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttctt                                >  1:1426304/1‑71 (MQ=255)
cTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttctt                                >  1:1274841/1‑71 (MQ=255)
cTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttctt                                >  1:1262195/1‑71 (MQ=255)
cTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttctt                                >  1:1207911/1‑71 (MQ=255)
cTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttct                                 >  1:1022379/1‑70 (MQ=255)
cTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttct                                 >  1:2214961/1‑70 (MQ=255)
cTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttct                                 >  1:2149297/1‑70 (MQ=255)
   cTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTccg                             >  1:256922/1‑71 (MQ=255)
   cTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTccg                             >  1:1109170/1‑71 (MQ=255)
        cGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTccgccg                          >  1:208237/1‑69 (MQ=255)
        cGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGa                         >  1:1926395/1‑70 (MQ=255)
        cGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGa                         >  1:2561299/1‑70 (MQ=255)
        cGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGa                         >  1:2721112/1‑70 (MQ=255)
        cGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGa                         >  1:2716137/1‑70 (MQ=255)
        cGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGa                         >  1:1994505/1‑70 (MQ=255)
        cGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGa                         >  1:2010233/1‑70 (MQ=255)
        cGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGa                         >  1:2699825/1‑70 (MQ=255)
        cGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGa                         >  1:2171167/1‑70 (MQ=255)
        cGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGa                         >  1:2184522/1‑70 (MQ=255)
        cGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGa                         >  1:2251082/1‑70 (MQ=255)
        cGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGa                         >  1:2253958/1‑70 (MQ=255)
        cGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGa                         >  1:2523057/1‑70 (MQ=255)
        cGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGa                         >  1:2549255/1‑70 (MQ=255)
        cGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGa                         >  1:2805704/1‑70 (MQ=255)
        cGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGa                         >  1:1869636/1‑70 (MQ=255)
        cGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGa                         >  1:284577/1‑70 (MQ=255)
        cGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGa                         >  1:1482551/1‑70 (MQ=255)
        cGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGa                         >  1:550686/1‑70 (MQ=255)
        cGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGa                         >  1:466001/1‑70 (MQ=255)
        cGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGa                         >  1:106969/1‑70 (MQ=255)
        cGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGa                         >  1:384805/1‑70 (MQ=255)
        cGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGa                         >  1:379072/1‑70 (MQ=255)
        cGTTTTTGCATTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGa                         >  1:2940814/1‑70 (MQ=255)
          tttttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAAtata                                            >  1:2696241/1‑49 (MQ=255)
          tttttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGaca                       >  1:1007088/1‑70 (MQ=255)
          tttttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGaca                       >  1:579330/1‑70 (MQ=255)
          tttttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGaca                       >  1:430384/1‑70 (MQ=255)
          tttttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGaca                       >  1:387442/1‑70 (MQ=255)
          tttttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGaca                       >  1:2647449/1‑70 (MQ=255)
          tttttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGaca                       >  1:3151571/1‑70 (MQ=255)
          tttttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGaca                       >  1:1106586/1‑70 (MQ=255)
          tttttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGaca                       >  1:2907256/1‑70 (MQ=255)
          tttttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGaca                       >  1:2894094/1‑70 (MQ=255)
          tttttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGaca                       >  1:2892887/1‑70 (MQ=255)
          tttttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGaca                       >  1:276626/1‑70 (MQ=255)
          tttttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGaca                       >  1:2783168/1‑70 (MQ=255)
          tttttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGaca                       >  1:1722527/1‑70 (MQ=255)
          tttttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGaca                       >  1:117267/1‑70 (MQ=255)
          tttttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGaca                       >  1:1331079/1‑70 (MQ=255)
          tttttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGaca                       >  1:1513520/1‑70 (MQ=255)
          tttttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGaca                       >  1:1580744/1‑70 (MQ=255)
          tttttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGaca                       >  1:1626491/1‑70 (MQ=255)
          tttttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGaca                       >  1:1801516/1‑70 (MQ=255)
          tttttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGaca                       >  1:1820165/1‑70 (MQ=255)
          tttttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGaca                       >  1:1873290/1‑70 (MQ=255)
          tttttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGaca                       >  1:197559/1‑70 (MQ=255)
          tttttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGac                        >  1:1004325/1‑69 (MQ=255)
          tttttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGACGaca                       >  1:2904443/1‑70 (MQ=255)
          catttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGaca                       >  1:1957182/3‑70 (MQ=255)
                       gCTTCGCCAGTTGCAG‑CCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTa        <  1:2973659/71‑1 (MQ=255)
                         ttCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTa        <  1:1946768/70‑1 (MQ=255)
                              cAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAt  <  1:3029574/71‑1 (MQ=255)
                              cAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAt  <  1:2618226/71‑1 (MQ=255)
                              cAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAt  <  1:1157327/71‑1 (MQ=255)
                              cAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAt  <  1:217681/71‑1 (MQ=255)
                              cAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAt  <  1:1144590/71‑1 (MQ=255)
                              cAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAt  <  1:441540/71‑1 (MQ=255)
                              cAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAt  <  1:1552901/71‑1 (MQ=255)
                              cAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAt  <  1:1578681/71‑1 (MQ=255)
                              cAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAt  <  1:2675623/71‑1 (MQ=255)
                              cAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAt  <  1:1624611/71‑1 (MQ=255)
                              cAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAt  <  1:3170740/71‑1 (MQ=255)
                              cAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAt  <  1:2693570/71‑1 (MQ=255)
                              cAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAt  <  1:3122599/71‑1 (MQ=255)
                              cAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAt  <  1:3102970/71‑1 (MQ=255)
                              cAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAt  <  1:3069078/71‑1 (MQ=255)
                              cAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAt  <  1:1123845/71‑1 (MQ=255)
                              cAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAt  <  1:2990009/71‑1 (MQ=255)
                              cAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAt  <  1:1647092/71‑1 (MQ=255)
                              cAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAt  <  1:2962737/71‑1 (MQ=255)
                              cAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAt  <  1:2943855/71‑1 (MQ=255)
                              cAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAt  <  1:1136372/71‑1 (MQ=255)
                              cAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAt  <  1:2549659/71‑1 (MQ=255)
                              cAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAt  <  1:1768557/71‑1 (MQ=255)
                              cAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAt  <  1:2777745/71‑1 (MQ=255)
                              cAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAt  <  1:290376/71‑1 (MQ=255)
                              cAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAt  <  1:1778616/71‑1 (MQ=255)
                              cAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAt  <  1:2093527/71‑1 (MQ=255)
                              cAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAt  <  1:2859048/71‑1 (MQ=255)
                              cAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAt  <  1:2027428/71‑1 (MQ=255)
                                              gTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAt  <  1:2771055/55‑1 (MQ=255)
                                                 |                                                   
CTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTATTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAT  >  minE/575367‑575467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A