Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F28 I3 R2
|
122 |
64.1 |
3058059 |
97.6% |
2984665 |
63.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
575,416 |
T→C |
N135S (AAT→AGT) |
ybjX ← |
conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 575,416 | 0 | T | C | 100.0%
| 182.6
/ NA
| 52 | N135S (AAT→AGT) | ybjX | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (33/19); total (33/19) |
GTCAGCTCAAGCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTATTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCATAATGGTAACGTA > minE/575350‑575479
|
gTCAGCTCAAGCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCaa < 1:1359067/71‑1 (MQ=255)
gTCAGCTCAAGCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCaa < 1:373966/71‑1 (MQ=255)
gTCAGCTCAAGCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCaa < 1:2525539/71‑1 (MQ=255)
gTCAGCTCAAGCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCa < 1:110348/70‑1 (MQ=255)
cAAGCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAg > 1:1278441/1‑71 (MQ=255)
cAAGCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAg > 1:1990468/1‑71 (MQ=255)
aaGCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGc > 1:815788/1‑71 (MQ=255)
gCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTc > 1:2408223/1‑59 (MQ=255)
gCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTg < 1:1325065/71‑1 (MQ=255)
gTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGaa > 1:899836/1‑71 (MQ=255)
gTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGaa > 1:2989753/1‑71 (MQ=255)
tGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGAAGCCCGGGGGTACTCAAATATAAGCTGAAt > 1:2409443/1‑71 (MQ=255)
gAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCaaa > 1:698213/1‑58 (MQ=255)
aaCTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttc > 1:2840477/1‑71 (MQ=255)
tGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttctt < 1:2581113/70‑1 (MQ=255)
gCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTcc > 1:2804996/1‑71 (MQ=255)
gCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTcc > 1:1803003/1‑71 (MQ=255)
cGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGGCCGGGGGTACTCAAATATAAGCTGAAttctt > 1:2491520/1‑66 (MQ=255)
cGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTccgc > 1:625564/1‑70 (MQ=255)
ccGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttctt < 1:2629986/64‑1 (MQ=255)
gTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGaca < 1:506017/71‑1 (MQ=255)
gTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGa > 1:1627787/1‑69 (MQ=255)
tttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGacata > 1:708622/1‑70 (MQ=255)
tGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCGGCCGacatacat > 1:20689/1‑71 (MQ=255)
cTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTcg < 1:110226/71‑1 (MQ=255)
ttCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTcgc > 1:2017588/1‑71 (MQ=255)
ttCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTcgc > 1:224441/1‑71 (MQ=255)
tCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTcgcg > 1:331157/1‑71 (MQ=255)
ccAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTcgcg > 1:1153022/1‑70 (MQ=255)
cTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTccgccg < 1:839828/53‑1 (MQ=255)
cTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTa < 1:387803/71‑1 (MQ=255)
tCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGaa > 1:1741702/1‑40 (MQ=255)
cAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCa > 1:772174/1‑70 (MQ=255)
gTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCATaa > 1:860175/1‑71 (MQ=255)
ttGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTa < 1:1520543/62‑1 (MQ=255)
ttGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCAt < 1:722787/68‑1 (MQ=255)
tGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTaa > 1:1359299/1‑62 (MQ=255)
cAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCATAATGgt < 1:1310129/71‑1 (MQ=255)
aGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACAtt > 1:1528464/1‑49 (MQ=255)
aGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACAtt > 1:2309779/1‑49 (MQ=255)
aGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCATAATGgta < 1:2497929/71‑1 (MQ=255)
aGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCATAATGgta < 1:948551/71‑1 (MQ=255)
gCCCGGGGGTACTCAAATATAAGCTGATTTCTTccgcc > 1:2601272/1‑38 (MQ=38)
gCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTc < 1:184987/49‑1 (MQ=255)
gCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCATAATGgtaa > 1:511250/1‑71 (MQ=255)
gCCCGGGGGAACTCAAATATAAGCTGAATTCTTCCGCCGacata > 1:2804441/1‑44 (MQ=255)
cccGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGacataca > 1:2609955/1‑45 (MQ=255)
cccGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTa > 1:2965086/1‑56 (MQ=255)
cccGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCATAATGgtaac < 1:605172/71‑1 (MQ=255)
cGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCATAATGgtaacg > 1:2308687/1‑70 (MQ=255)
ggggTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCATAATGgtaacgta < 1:1625149/70‑1 (MQ=255)
gggTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCATAATGgtaac > 1:991695/1‑66 (MQ=255)
|
GTCAGCTCAAGCGTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTATTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCGCGGAGTAACGCATAATGGTAACGTA > minE/575350‑575479
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
