Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	122	92.7	4382561	94.3%	4132755	66.1

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	575,416	T→C	N135S (AAT→AGT)	ybjX ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	575,416	0	T	C	100.0%	111.5 / NA	30	N135S (AAT→AGT)	ybjX	conserved hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/16); total (14/16)

GTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTATTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCG  >  minE/575362‑575454

gTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGaa                        <  1:95658/71‑1 (MQ=255)

gTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGaa                        <  1:2353013/71‑1 (MQ=255)

gTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGaa                        <  1:237867/71‑1 (MQ=255)

gTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGaa                        <  1:4006485/71‑1 (MQ=255)

gTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGaa                        <  1:3582488/71‑1 (MQ=255)

gTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGaa                        <  1:2615217/71‑1 (MQ=255)

gTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGaa                        <  1:3261918/71‑1 (MQ=255)

gTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGaa                        <  1:3112523/71‑1 (MQ=255)

   aaCTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttc                     >  1:3219132/1‑71 (MQ=255)

   aaCTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttc                     >  1:869968/1‑71 (MQ=255)

   aaCTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttc                     >  1:574174/1‑71 (MQ=255)

   aaCTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttc                     >  1:4284395/1‑71 (MQ=255)

   aaCTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttc                     >  1:3588207/1‑71 (MQ=255)

   aaCTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttc                     >  1:2692881/1‑71 (MQ=255)

   aaCTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttc                     >  1:2483613/1‑71 (MQ=255)

   aaCTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAt                       >  1:1789582/1‑69 (MQ=255)

               tttttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGaca          >  1:2883176/1‑70 (MQ=255)

                 tttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttc                     >  1:1650175/1‑57 (MQ=255)

                 tttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttc                     >  1:2458425/1‑57 (MQ=255)

                 tttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttc                     >  1:2181817/1‑57 (MQ=255)

                 tttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttc                     >  1:791414/1‑57 (MQ=255)

                 tttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttc                     >  1:203170/1‑57 (MQ=255)

                   tGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGacataca      <  1:3217362/70‑1 (MQ=255)

                    gCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGacataca      <  1:4147938/69‑1 (MQ=255)

                      cTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTcg  <  1:4017219/71‑1 (MQ=255)

                      cTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTcg  <  1:857666/71‑1 (MQ=255)

                      cTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTcg  <  1:500765/71‑1 (MQ=255)

                      cTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTcg  <  1:3961477/71‑1 (MQ=255)

                      cTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTcg  <  1:2587719/71‑1 (MQ=255)

                      cTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCACCGACATACATTcg  <  1:1654788/71‑1 (MQ=255)

GTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTATTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCG  >  minE/575362‑575454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

N/A