Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I1 R2 146 74.7 3299319 84.8% 2797822 66.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 1,081,858 T→C N104S (AAC→AGC)  ydhY ← predicted 4Fe‑4S ferridoxin‑type protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,081,8580TC100.0% 229.5 / NA 62N104S (AAC→AGC) ydhYpredicted 4Fe‑4S ferridoxin‑type protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/41);  total (21/41)

TCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGTTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTC  >  minE/1081807‑1081908
                                                   |                                                  
tCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGATCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 <  1:2513545/71‑1 (MQ=255)
tCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 <  1:345196/71‑1 (MQ=255)
tCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 <  1:1070117/71‑1 (MQ=255)
tCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 <  1:2919989/71‑1 (MQ=255)
tCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 <  1:2251954/71‑1 (MQ=255)
tCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 <  1:2167212/71‑1 (MQ=255)
tCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 <  1:2152255/71‑1 (MQ=255)
tCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 <  1:1881531/71‑1 (MQ=255)
tCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 <  1:1778080/71‑1 (MQ=255)
tCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 <  1:1539307/71‑1 (MQ=255)
tCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 <  1:1484460/71‑1 (MQ=255)
gccGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 <  1:1291372/70‑1 (MQ=255)
  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGaaa                                      >  1:1051662/1‑64 (MQ=255)
  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 >  1:1483874/1‑69 (MQ=255)
  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 >  1:192928/1‑69 (MQ=255)
  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:1072571/1‑71 (MQ=255)
  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:966994/1‑71 (MQ=255)
  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:894002/1‑71 (MQ=255)
  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:488858/1‑71 (MQ=255)
  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:3060318/1‑71 (MQ=255)
  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:2948180/1‑71 (MQ=255)
  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:1072063/1‑71 (MQ=255)
  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:2858505/1‑71 (MQ=255)
  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:2689699/1‑71 (MQ=255)
  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:2678820/1‑71 (MQ=255)
  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:2553828/1‑71 (MQ=255)
  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:2472351/1‑71 (MQ=255)
  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:1910981/1‑71 (MQ=255)
  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:1793062/1‑71 (MQ=255)
  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:1907098/1‑71 (MQ=255)
  cgcgGTATAGTTGAGATCACCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:2391661/1‑71 (MQ=255)
      gTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATg                           <  1:3095931/71‑1 (MQ=255)
      gTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATg                           <  1:407877/71‑1 (MQ=255)
        ataGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGa                         >  1:1551496/1‑71 (MQ=255)
         taGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGAt                        >  1:1164925/1‑71 (MQ=255)
            ttGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 <  1:2371542/59‑1 (MQ=255)
                     ccATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 <  1:804880/50‑1 (MQ=255)
                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACTGGAGAAGAATGTc  <  1:859298/71‑1 (MQ=255)
                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:832117/71‑1 (MQ=255)
                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:1715129/71‑1 (MQ=255)
                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:1681653/71‑1 (MQ=255)
                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:870409/71‑1 (MQ=255)
                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:1605423/71‑1 (MQ=255)
                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:4047/71‑1 (MQ=255)
                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:963139/71‑1 (MQ=255)
                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:1017770/71‑1 (MQ=255)
                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:3252731/71‑1 (MQ=255)
                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:987165/71‑1 (MQ=255)
                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:3091487/71‑1 (MQ=255)
                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:1692828/71‑1 (MQ=255)
                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:1750451/71‑1 (MQ=255)
                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:1413159/71‑1 (MQ=255)
                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:1166546/71‑1 (MQ=255)
                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:2656853/71‑1 (MQ=255)
                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:1894925/71‑1 (MQ=255)
                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:1486650/71‑1 (MQ=255)
                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:2419255/71‑1 (MQ=255)
                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:2290872/71‑1 (MQ=255)
                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:1073310/71‑1 (MQ=255)
                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:2087898/71‑1 (MQ=255)
                                 ccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATTGATTTTGATACGGGAGAAGAATGTc  <  1:1087568/69‑1 (MQ=255)
                                 ccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:884064/69‑1 (MQ=255)
                                                   |                                                  
TCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGTTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTC  >  minE/1081807‑1081908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A