Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F8 I0 R2
|
422 |
46.8 |
3726540 |
94.7% |
3529033 |
61.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,373,368 |
G→A |
86.3% |
R480H (CGT→CAT) |
ycjM → |
predicted glucosyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,373,368 | 0 | G | A | 86.3%
| 204.9
/ 23.2
| 80 | R480H (CGT→CAT) | ycjM | predicted glucosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (9/2); new base A (38/31); total (47/33) |
| Fisher's exact test for biased strand distribution p-value = 1.13e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 |
AACTGAACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGT > W3110S.gb/1373302‑1373431
|
aaCTGAACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTtcatcg > 1:2367918/1‑70 (MQ=255)
aaCTGAACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTtcatcg > 1:2363746/1‑70 (MQ=255)
aTGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAAc < 1:18886/71‑1 (MQ=255)
aTGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAAc < 1:815806/71‑1 (MQ=255)
aTGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAAc < 1:1781461/71‑1 (MQ=255)
aTGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAAc < 1:1388317/71‑1 (MQ=255)
tGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACg < 1:983694/71‑1 (MQ=255)
gAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGACATAACg < 1:1066540/70‑1 (MQ=255)
ttAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATcc > 1:607099/1‑71 (MQ=255)
aaGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCg > 1:524632/1‑70 (MQ=255)
aaGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCg > 1:3637682/1‑70 (MQ=255)
aaGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCg > 1:3584655/1‑70 (MQ=255)
aaGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCg > 1:2754425/1‑70 (MQ=255)
tataTCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATACtttt < 1:1628087/71‑1 (MQ=255)
tataTCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAAtttt < 1:148556/71‑1 (MQ=255)
tataTCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAAtttt < 1:1632472/71‑1 (MQ=255)
tatCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACg < 1:929643/46‑1 (MQ=255)
tatCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTAc < 1:2484503/71‑1 (MQ=255)
tatCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGACTTTCATCCGGATAATAATTTTAc > 1:1588769/1‑71 (MQ=255)
atCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTAc < 1:3648689/70‑1 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCa < 1:2271483/51‑1 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:268263/1‑71 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:1925070/1‑71 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCCGCCATAACGAGTTTCATCCGGATAATAATTTTAcc > 1:3279049/1‑70 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAAttt > 1:3568652/1‑66 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTAcc < 1:1924129/70‑1 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTAcc < 1:1382268/70‑1 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTAcc > 1:841482/1‑70 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTAcc < 1:3532695/70‑1 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTAcc < 1:3225411/70‑1 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTAcc < 1:923734/70‑1 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTAcc < 1:928044/70‑1 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTAcc < 1:2969095/70‑1 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTAcc < 1:2757169/70‑1 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:1521955/1‑71 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:1210470/1‑71 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:583650/1‑71 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa < 1:117364/71‑1 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:1387663/1‑71 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:1952742/1‑71 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:145296/1‑71 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:1982731/1‑71 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:3285400/1‑71 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:3241495/1‑71 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:3012091/1‑71 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:2529208/1‑71 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:2974575/1‑71 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:2621248/1‑71 (MQ=255)
aTGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCAt > 1:3012686/1‑70 (MQ=255)
aTGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCAtt > 1:36361/1‑71 (MQ=255)
aTGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCAtt > 1:2672668/1‑71 (MQ=255)
aTGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCAtt > 1:541294/1‑71 (MQ=255)
aTGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCAt > 1:3162278/1‑70 (MQ=255)
ttGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCAtt < 1:2598273/66‑1 (MQ=255)
ttGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCAtt < 1:265238/66‑1 (MQ=255)
ttGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCAtt < 1:3656146/66‑1 (MQ=255)
ttGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCAtt < 1:447952/66‑1 (MQ=255)
gTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGa > 1:735006/1‑71 (MQ=255)
gTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGa > 1:737899/1‑71 (MQ=255)
cgcgTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATaataat > 1:3446528/1‑53 (MQ=255)
cgcgTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATaataat > 1:2267201/1‑53 (MQ=255)
cgcgTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATaataat > 1:934357/1‑53 (MQ=255)
gcgTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGAtt < 1:1021863/70‑1 (MQ=255)
gcgTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGAtt > 1:361537/1‑70 (MQ=255)
gcgTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGAtt < 1:3290753/70‑1 (MQ=255)
gTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAAt < 1:2416610/71‑1 (MQ=255)
tttAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGat < 1:2422629/43‑1 (MQ=255)
tAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCa > 1:3412000/1‑71 (MQ=255)
tAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCa > 1:1499700/1‑71 (MQ=255)
tAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCa > 1:1557615/1‑71 (MQ=255)
tAATTACACTTCATCGCAGCCATAACGAGTTTCAt > 1:2253832/1‑35 (MQ=255)
tAATTACACTTCATCGCAGCCATAACGAGTTTCAt > 1:967426/1‑35 (MQ=255)
tAATTACACTTCATCGCAGCCATAACGAGTTTCAt > 1:1103474/1‑35 (MQ=255)
tAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGataa > 1:1026658/1‑43 (MQ=255)
tAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGataa > 1:450894/1‑43 (MQ=255)
tAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGataa > 1:16049/1‑43 (MQ=255)
tAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGataa > 1:2331512/1‑43 (MQ=255)
tAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGataa > 1:1901521/1‑43 (MQ=255)
tAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCa > 1:1408886/1‑71 (MQ=255)
cacTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGt < 1:315089/70‑1 (MQ=255)
cacTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGt < 1:3154501/70‑1 (MQ=255)
cacTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGt < 1:184169/70‑1 (MQ=255)
|
AACTGAACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGT > W3110S.gb/1373302‑1373431
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
