Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F17 I0 R2
|
276 |
45.3 |
3729697 |
93.0% |
3468618 |
60.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,373,368 |
G→A |
86.1% |
R480H (CGT→CAT) |
ycjM → |
predicted glucosyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,373,368 | 0 | G | A | 86.1%
| 217.2
/ 26.4
| 86 | R480H (CGT→CAT) | ycjM | predicted glucosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (10/2); new base A (32/42); total (42/44) |
| Fisher's exact test for biased strand distribution p-value = 1.27e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01 |
AACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGT > W3110S.gb/1373307‑1373431
|
aaCGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGc > 1:1706034/1‑69 (MQ=255)
aaCGATGAAGCTACATAAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGc > 1:1437760/1‑69 (MQ=255)
gATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCAt < 1:2256676/69‑1 (MQ=255)
gATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCAt < 1:360851/69‑1 (MQ=255)
gAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGc < 1:55246/63‑1 (MQ=255)
gAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGc < 1:2583419/63‑1 (MQ=255)
gAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGc < 1:3411221/63‑1 (MQ=255)
gAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGc < 1:3424567/63‑1 (MQ=255)
aaGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACg > 1:2956991/1‑69 (MQ=255)
aaGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACg > 1:36588/1‑69 (MQ=255)
gCTACATTAAGGCATGCGGTATATCATGAGTTGTCTCGTTTAATTACACTTCATCGCAGCCATAACGAg > 1:535140/1‑69 (MQ=255)
gCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGa < 1:2715302/68‑1 (MQ=255)
gCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGa < 1:2225242/68‑1 (MQ=255)
cTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGt < 1:2136974/69‑1 (MQ=255)
aCATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGttt > 1:29647/1‑69 (MQ=255)
aaGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCATCCATAACGat < 1:2077810/61‑2 (MQ=255)
aaGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAg < 1:3562450/61‑1 (MQ=255)
aaGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATc > 1:181443/1‑68 (MQ=255)
cATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGat < 1:977155/69‑1 (MQ=255)
tGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGc < 1:487674/46‑1 (MQ=255)
tGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGc < 1:1201239/46‑1 (MQ=255)
cGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGataata > 1:1059351/1‑69 (MQ=255)
cGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGataata > 1:273712/1‑69 (MQ=255)
cGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGataata > 1:2118109/1‑69 (MQ=255)
tatCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAAtttt > 1:3147576/1‑69 (MQ=255)
tatCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAAtttt > 1:2042688/1‑69 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTAc > 1:1437917/1‑69 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCAt < 1:3613411/40‑1 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCAt < 1:1203367/40‑1 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCAt < 1:1495578/40‑1 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCAt < 1:1111422/40‑1 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCAt < 1:1907000/40‑1 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCAt < 1:68576/40‑1 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAg > 1:3519519/1‑46 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAg < 1:2485969/46‑1 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTc > 1:2467343/1‑50 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGTATAATAATTTTAc > 1:1321713/1‑69 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGAtaataa < 1:2103622/63‑1 (MQ=255)
tCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTAc < 1:2360217/69‑1 (MQ=255)
aTGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCa > 1:1417424/1‑37 (MQ=255)
aTGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:566261/1‑69 (MQ=255)
aTGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:1910584/1‑69 (MQ=255)
aTGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCa > 1:220251/1‑69 (MQ=255)
gAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCAtt < 1:400737/69‑1 (MQ=255)
gAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCAtt < 1:3281856/69‑1 (MQ=255)
gAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCAtt < 1:2251545/69‑1 (MQ=255)
gTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAg < 1:1551629/40‑1 (MQ=255)
gTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTAc > 1:455582/1‑60 (MQ=255)
gTCGCGTTTAATTACACTTCATCGCAGCCATAACGAg > 1:1387686/1‑37 (MQ=255)
gTCGCGTTTAATTACACTTCATCGCAGCCATAACGAg > 1:3551999/1‑37 (MQ=255)
gTCGCGTTTAATTACACTTCATCGCAGCCATAACGAg > 1:1907202/1‑37 (MQ=255)
gTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAAtttt < 1:2243385/58‑1 (MQ=255)
gTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATAc < 1:3133102/69‑1 (MQ=255)
gTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATAc < 1:2449742/69‑1 (MQ=255)
gTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATAc < 1:2585642/69‑1 (MQ=255)
gTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATAc > 1:146042/1‑69 (MQ=255)
gTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATAc < 1:2721966/69‑1 (MQ=255)
gTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATAc < 1:2021034/69‑1 (MQ=255)
gTCGCGGTTAATTACACTTCATCGCAGCCATAACGAg > 1:474538/1‑37 (MQ=37)
gTCGAGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAAtttt < 1:99768/58‑1 (MQ=255)
cgcgTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACg > 1:1764655/1‑68 (MQ=255)
tAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTa < 1:3332676/66‑1 (MQ=255)
tAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAAtt > 1:1088713/1‑69 (MQ=255)
tAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAAtt > 1:2227062/1‑69 (MQ=255)
tAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGa < 1:1069484/40‑1 (MQ=255)
tAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAAtt > 1:1843564/1‑69 (MQ=255)
tAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAAtt > 1:2019438/1‑69 (MQ=255)
tAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAAtt > 1:255528/1‑69 (MQ=255)
acacTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATc > 1:1697199/1‑68 (MQ=255)
acacTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATc > 1:358923/1‑68 (MQ=255)
acacTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATc > 1:3600004/1‑68 (MQ=255)
acacTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATc > 1:3315715/1‑68 (MQ=255)
acacTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATc > 1:3706267/1‑68 (MQ=255)
acacTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATc > 1:1649534/1‑68 (MQ=255)
acacTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATc > 1:683994/1‑68 (MQ=255)
acacTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATc > 1:1128651/1‑68 (MQ=255)
cacTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATAc < 1:2597563/55‑1 (MQ=255)
cacTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATAc > 1:306387/1‑55 (MQ=255)
cacTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATAc > 1:3670942/1‑55 (MQ=255)
cacTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATAc < 1:1354614/55‑1 (MQ=255)
cacTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATAc < 1:1309071/55‑1 (MQ=255)
acTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCg < 1:1021627/68‑1 (MQ=255)
cTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGt < 1:533922/68‑1 (MQ=255)
cTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGt < 1:2075470/68‑1 (MQ=255)
cTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGt < 1:2271653/68‑1 (MQ=255)
cTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGt < 1:862664/68‑1 (MQ=255)
tcgtcgCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCAtt < 1:3262139/46‑1 (MQ=255)
tcgtcgCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGt < 1:1859874/66‑1 (MQ=255)
tcgtcgCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGt > 1:3382580/1‑66 (MQ=255)
cgtcgCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCa < 1:1130644/60‑1 (MQ=255)
cgtcgCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCa < 1:692754/60‑1 (MQ=255)
|
AACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGT > W3110S.gb/1373307‑1373431
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
