Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F28 I2 R1
|
28 |
4.9 |
280516 |
80.6% |
226095 |
64.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
2,523,040 |
T→C |
K225K (AAA→AAG) |
dinD ← |
DNA‑damage‑inducible protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,523,040 | 0 | T | C | 100.0%
| 15.7
/ NA
| 6 | K225K (AAA→AAG) | dinD | DNA‑damage‑inducible protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (3/3); total (3/3) |
CCAAGTTCCTGAATGGTTTGCCTCACCTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGTTTTGAATTCACGCCATCCCGCTTGAGTTTTTCTTCT > minE/2522975‑2523076
|
ccAAGTTCCTGAATGGTTTGCCTCACCTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGCTTTGa < 1:174578/71‑1 (MQ=255)
ccAAGTTCCTGAATGGTTTGCCTCACCTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGCTTTGa < 1:266903/71‑1 (MQ=255)
tGCCTCACCTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGCTTTGAATTCACGCCATCCCGCtt > 1:225811/1‑71 (MQ=255)
cTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGCTTTGAATTCACGCCATCCCGCTTGAGttttt > 1:124068/1‑71 (MQ=255)
tgtgtgGTGTTTGCTTGCTGCTTTGAATTCACGCCATCCCGCTTGAGtt < 1:131266/49‑1 (MQ=255)
gtgtgGTGTTTGCTTGCTGCTTTGAATTCACGCCATCCCGCTTGAGTTTTtcttct > 1:25106/1‑56 (MQ=255)
|
CCAAGTTCCTGAATGGTTTGCCTCACCTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGTTTTGAATTCACGCCATCCCGCTTGAGTTTTTCTTCT > minE/2522975‑2523076
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
