Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R2
|
356 |
55.1 |
4139924 |
95.4% |
3949487 |
63.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
556,858 |
A→T |
L36Q (CTG→CAG) |
folD ← |
bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 556,858 | 0 | A | T | 100.0%
| 167.0
/ NA
| 55 | L36Q (CTG→CAG) | folD | bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (24/31); total (24/31) |
GCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTA‑CCCACCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCA > W3110S.gb/556795‑556914
|
gCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTA‑CCCACCAGCACAACGGCCTGTCCTgg > 1:2866512/1‑71 (MQ=255)
gCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTA‑CCCACCAGCACAACGGCCTGTCCTgg > 1:1401040/1‑71 (MQ=255)
cGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTA‑CCCACCAGCACAACGGCCTGTCCTGGTGCCCg < 1:2473592/71‑1 (MQ=255)
cGACATAAATTTGCGATGCAGGGTTACTA‑CCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGc < 1:2211595/70‑1 (MQ=255)
cGACATAAATTTGCGATGCAGGGTTACTA‑CCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGc < 1:563339/70‑1 (MQ=255)
aCATATATTTGCGATGCAGGGTTACTA‑CCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc < 1:28085/71‑1 (MQ=255)
aCATAAATTTGCGATGCAGGGTTACTA‑CCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc < 1:3511254/71‑1 (MQ=255)
aCATAAATTTGCGATGCAGGGTTACTA‑CCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc < 1:198860/71‑1 (MQ=255)
aCATAAATTTGCGATGCAGGGTTACTA‑CCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc < 1:3854737/71‑1 (MQ=255)
aCATAAATTTGCGATGCAGGGTTACTA‑CCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc < 1:366248/71‑1 (MQ=255)
aCATAAATTTGCGATGCAGGGTTACTA‑CCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc < 1:3657579/71‑1 (MQ=255)
aTAAATTTGCGATGCAGGGTTACTA‑CCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCaa < 1:292213/71‑1 (MQ=255)
aTAAATTTGCGATGCAGGGTTACTA‑CCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCaa < 1:1492075/71‑1 (MQ=255)
aTAAATTTGCGATGCAGGGTTACTA‑CCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGACCGGCTGCaa < 1:3486244/71‑1 (MQ=255)
tAAATTTGCGATGCAGGGTTACTA‑CCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc < 1:1627262/68‑1 (MQ=255)
tAAATTTGCGATGCAGGGTTACTA‑CCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc < 1:3702419/68‑1 (MQ=255)
tAAATTTGCGATGCAGGGTTACTA‑CCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc < 1:623934/68‑1 (MQ=255)
tAAATTTGCGATGCAGGGTTACTA‑CCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCaa < 1:3488242/70‑1 (MQ=255)
tAAATTTGCGATGCAGGGTTACTA‑CCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCaa < 1:881151/70‑1 (MQ=255)
tAAATTTGCGATGCAGGGTTACTA‑CCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCaa < 1:2527168/70‑1 (MQ=255)
tAAATTTGCGATGCAGGGTTACTA‑CCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCaa < 1:1947185/70‑1 (MQ=255)
tAAATTTGCGATGCAGGGTTACTA‑CCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCaa < 1:1312506/70‑1 (MQ=255)
tAAATTTGCGATGCAGGGTTACTA‑CCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCaa < 1:1307632/70‑1 (MQ=255)
aaTTTGCGATGCAGGGTTACTA‑CCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCaa > 1:2642416/1‑68 (MQ=255)
aaTTTGCGATGCAGGGTTACTA‑CCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCaa > 1:3984881/1‑68 (MQ=255)
aaTTTGCGATGCAGGGTTACTA‑CCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCaa > 1:1561674/1‑68 (MQ=255)
gCGATGCAGGGTTACTACCCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATAcgcg < 1:1385447/70‑1 (MQ=255)
gCGATGCAGGGTTACTACCCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGGCCGGCTGCAATAcgcg < 1:1803024/70‑1 (MQ=255)
cAGGGTTACTA‑CCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc < 1:441804/55‑1 (MQ=255)
ttACTA‑CCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCaa < 1:965020/52‑1 (MQ=255)
tacta‑cCCACCAGCACAACGGCCTGTCCTGGTGCCCGTAGTCCGGCTGCAATACGCGCCTGAACttttt > 1:3015854/1‑69 (MQ=255)
tacta‑cCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGc < 1:3343443/46‑1 (MQ=255)
tacta‑cCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttttt > 1:4093150/1‑69 (MQ=255)
acta‑cCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAg > 1:1428277/1‑71 (MQ=255)
cta‑cCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGc < 1:3474920/71‑1 (MQ=255)
caccaGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACtt > 1:811647/1‑71 (MQ=255)
ccaGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCa < 1:593081/65‑1 (MQ=255)
ccaGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCa < 1:426033/65‑1 (MQ=255)
ccaGCACAACGGCCTGTCCTGGCGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCa < 1:510254/65‑1 (MQ=255)
caGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc < 1:736316/39‑1 (MQ=255)
aGCACAACGGCCTGTCCTGGTGCCCTCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAAc > 1:1236525/1‑65 (MQ=255)
aGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAAc > 1:4005636/1‑65 (MQ=255)
aGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAAc > 1:593650/1‑65 (MQ=255)
aGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAAc > 1:3759908/1‑65 (MQ=255)
aGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAAc > 1:371376/1‑65 (MQ=255)
aGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAAc > 1:3530016/1‑65 (MQ=255)
aGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAAc > 1:1255347/1‑65 (MQ=255)
aGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAAc > 1:944408/1‑65 (MQ=255)
aGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTc > 1:1690556/1‑68 (MQ=255)
aGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCa > 1:4092668/1‑69 (MQ=255)
aGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCa > 1:1934058/1‑69 (MQ=255)
aGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCa > 1:217024/1‑69 (MQ=255)
aGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCa > 1:2872308/1‑69 (MQ=255)
aGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCa > 1:857628/1‑69 (MQ=255)
aGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCa > 1:3507421/1‑69 (MQ=255)
|
GCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTA‑CCCACCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCA > W3110S.gb/556795‑556914
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
