Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R2
|
356 |
55.1 |
4139924 |
95.4% |
3949487 |
63.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,173,170 |
C→T |
R791H (CGT→CAT) |
mfd ← |
transcription‑repair coupling factor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,173,170 | 0 | C | T | 100.0%
| 122.8
/ NA
| 41 | R791H (CGT→CAT) | mfd | transcription‑repair coupling factor |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (12/29); total (12/29) |
ATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGACGGGCGGGCGGC > W3110S.gb/1173100‑1173208
|
ataataAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCAc > 1:3154592/1‑71 (MQ=255)
aCCTGTCCTCC‑CGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAg < 1:1854106/71‑1 (MQ=255)
aCCTGTCCTCC‑CGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAg < 1:1849331/71‑1 (MQ=255)
ccTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAg < 1:3726231/71‑1 (MQ=255)
ccTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAg < 1:3749101/71‑1 (MQ=255)
ccTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAg < 1:4094155/71‑1 (MQ=255)
ccTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAg < 1:3053029/71‑1 (MQ=255)
ccTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAg < 1:430028/71‑1 (MQ=255)
ccTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAg < 1:1785226/71‑1 (MQ=255)
ccTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAg < 1:1780998/71‑1 (MQ=255)
ccTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAg < 1:3496209/71‑1 (MQ=255)
ccTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAg < 1:820625/71‑1 (MQ=255)
ccTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAg < 1:3673070/71‑1 (MQ=255)
ccTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAg < 1:1045100/71‑1 (MQ=255)
cTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAgg > 1:2599499/1‑71 (MQ=255)
cTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAgg > 1:2205308/1‑71 (MQ=255)
cTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAgg > 1:222620/1‑71 (MQ=255)
tcctccGCGCAAAATTCCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGt < 1:1471066/69‑1 (MQ=255)
cgcgCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGTTTTAACt < 1:3367020/71‑1 (MQ=255)
gcgcAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGAGAAAGGTTTTAACt < 1:2630241/70‑1 (MQ=255)
gcAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGt < 1:1178619/61‑1 (MQ=255)
aTTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCTTACTCATGGACAAAGGTTTTAACTGCCAgacg < 1:3220609/71‑1 (MQ=255)
aTTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAgacg < 1:2669123/71‑1 (MQ=255)
aTTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAgacg < 1:1976242/71‑1 (MQ=255)
gCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGACGAcgggcg < 1:3814062/71‑1 (MQ=255)
gCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGACGAcgggcg < 1:3854379/71‑1 (MQ=255)
gCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGACGAcgggcg < 1:3082103/71‑1 (MQ=255)
gCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGACGAcgggcg < 1:1818835/71‑1 (MQ=255)
gCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGACGAcgggcg < 1:178029/71‑1 (MQ=255)
gCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGACGAcgggcg < 1:3589449/71‑1 (MQ=255)
gCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGACGAcgggcg < 1:1610705/71‑1 (MQ=255)
cAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGACGAcgggcg < 1:1792555/70‑1 (MQ=255)
tCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGACGACGGGCGggcggc < 1:2107413/71‑1 (MQ=255)
aTGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAgacgac > 1:191850/1‑43 (MQ=255)
aTGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGacgacg > 1:974635/1‑44 (MQ=255)
aTGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGacgacg > 1:3022964/1‑44 (MQ=255)
aTGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGacgacg > 1:2298651/1‑44 (MQ=255)
aTGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGacgacg > 1:2146298/1‑44 (MQ=255)
aTGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGacgacg > 1:1945452/1‑44 (MQ=255)
aTGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGacgacg > 1:1837704/1‑44 (MQ=255)
aTGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGacgacg > 1:1660462/1‑44 (MQ=255)
aTGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGacgacg > 1:1396907/1‑44 (MQ=255)
|
ATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGACGGGCGGGCGGC > W3110S.gb/1173100‑1173208
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
