Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I2 R2	356	55.1	4139924	95.4%	3949487	63.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,401,010	T→C	K77K (AAA→AAG)	fnr ←	DNA‑binding transcriptional dual regulator, global regulator of anaerobic growth

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,401,010	0	T	C	100.0%	199.5 / NA	56	K77K (AAA→AAG)	fnr	DNA‑binding transcriptional dual regulator, global regulator of anaerobic growth
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/34); total (22/34)

CTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCG  >  W3110S.gb/1400955‑1401071

cTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAg                                                >  1:2038622/1‑71 (MQ=255)

cTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAg                                                <  1:62681/71‑1 (MQ=255)

cTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAg                                                >  1:2820477/1‑71 (MQ=255)

cTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAg                                                >  1:1396278/1‑71 (MQ=255)

cTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAg                                                >  1:4066075/1‑71 (MQ=255)

cTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAg                                                <  1:4004111/71‑1 (MQ=255)

cTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAg                                                <  1:1907888/71‑1 (MQ=255)

cTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAg                                                <  1:3872318/71‑1 (MQ=255)

cTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAg                                                <  1:2557102/71‑1 (MQ=255)

cTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAg                                                <  1:1719780/71‑1 (MQ=255)

cTGCTAAATGGAAACCAGTGATTTGCTCGTAGCCTTGATCAGTGATGGTATAACTCTTAATCGTACCGGa                                                 >  1:1645575/1‑70 (MQ=255)

 tGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAg                                                <  1:48077/70‑1 (MQ=255)

   cTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCg                                              <  1:2364184/70‑1 (MQ=255)

   cTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCg                                              <  1:2229437/70‑1 (MQ=255)

   cTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCg                                              <  1:547967/70‑1 (MQ=255)

   cTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCg                                              <  1:1310372/70‑1 (MQ=255)

   cTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCg                                              <  1:1956560/70‑1 (MQ=255)

   cTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCg                                              <  1:3295277/70‑1 (MQ=255)

   cTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGCATAACTCTTAATCGTACCGGAGCg                                              <  1:4119992/70‑1 (MQ=255)

     aaaTGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTa                                                      >  1:3732492/1‑60 (MQ=255)

     aaaTGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTa                                                      >  1:3817226/1‑60 (MQ=255)

        tGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATgg                                         >  1:1910567/1‑70 (MQ=255)

        tGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATgg                                         >  1:2303364/1‑70 (MQ=255)

         ggAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAg                                                <  1:3400038/62‑1 (MQ=255)

            aaCCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAg                                                <  1:2341316/59‑1 (MQ=255)

               cAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAg                                                <  1:2111823/56‑1 (MQ=255)

                 gTGATTTGCTCGTCGCCTTTCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGa                               <  1:1895196/71‑1 (MQ=255)

                 gTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGa                               <  1:1768321/71‑1 (MQ=255)

                 gTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGa                               <  1:2203478/71‑1 (MQ=255)

                 gTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGa                               <  1:3523372/71‑1 (MQ=255)

                 gTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGa                               <  1:1562309/71‑1 (MQ=255)

                 gTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGa                               <  1:2957605/71‑1 (MQ=255)

                 gTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGa                               <  1:1507716/71‑1 (MQ=255)

                 gTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGa                               <  1:3289612/71‑1 (MQ=255)

                 gTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGa                               <  1:1508081/71‑1 (MQ=255)

                    aTTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGct                                <  1:2023461/67‑2 (MQ=255)

                        gCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAg                         <  1:317274/70‑1 (MQ=255)

                        gCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAg                         <  1:3509453/70‑1 (MQ=255)

                        gCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAg                         <  1:2578989/70‑1 (MQ=255)

                        gCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAg                         <  1:2149860/70‑1 (MQ=255)

                        gCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAg                         <  1:2291690/70‑1 (MQ=255)

                               gCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCAc                 >  1:1011512/1‑71 (MQ=255)

                               gCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCAc                 >  1:1505134/1‑71 (MQ=255)

                               gCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCAc                 >  1:1701049/1‑71 (MQ=255)

                               gCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCAc                 >  1:3690148/1‑71 (MQ=255)

                               gCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCAc                 >  1:3636099/1‑71 (MQ=255)

                               gCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCAc                 >  1:3513515/1‑71 (MQ=255)

                               gCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCAc                 >  1:2006504/1‑71 (MQ=255)

                               gCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCAc                 >  1:3289568/1‑71 (MQ=255)

                               gCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCAc                 >  1:2798857/1‑71 (MQ=255)

                               gCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGAATAAAGCGATTTAAGTTCATCAc                 >  1:1167211/1‑71 (MQ=255)

                                       cAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGGGa                               <  1:1113947/49‑1 (MQ=255)

                                        aGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTa         <  1:463092/70‑1 (MQ=255)

                                              ggTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCg  >  1:3944863/1‑71 (MQ=255)

                                              ggTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCg  >  1:2994289/1‑71 (MQ=255)

                                              ggTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCg  >  1:940281/1‑71 (MQ=255)

CTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCG  >  W3110S.gb/1400955‑1401071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A