Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R2
|
356 |
55.1 |
4139924 |
95.4% |
3949487 |
63.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,821,480 |
T→C |
T316A (ACC→GCC) |
recA ← |
DNA strand exchange and recombination protein with protease and nuclease activity |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,821,480 | 0 | T | C | 100.0%
| 225.4
/ NA
| 65 | T316A (ACC→GCC) | recA | DNA strand exchange and recombination protein with protease and nuclease activity |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (7/58); total (7/58) |
GCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACC > W3110S.gb/2821420‑2821540
|
gCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa < 1:1144377/71‑1 (MQ=255)
gCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa < 1:2368048/71‑1 (MQ=255)
gCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa < 1:24702/71‑1 (MQ=255)
gCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa < 1:3381942/71‑1 (MQ=255)
gCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa < 1:3615933/71‑1 (MQ=255)
gCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa < 1:2011638/71‑1 (MQ=255)
gCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa < 1:1990017/71‑1 (MQ=255)
gCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa < 1:3944013/71‑1 (MQ=255)
gCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa < 1:3232126/71‑1 (MQ=255)
gCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa < 1:3110924/71‑1 (MQ=255)
gCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa < 1:1396346/71‑1 (MQ=255)
gCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa < 1:3174194/71‑1 (MQ=255)
gCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa < 1:1167358/71‑1 (MQ=255)
gCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCCGCTTCCGGGTTa < 1:1969154/71‑1 (MQ=255)
ttGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCt < 1:1593271/71‑1 (MQ=255)
ttGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCt < 1:1680099/71‑1 (MQ=255)
ttGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCt < 1:3081508/71‑1 (MQ=255)
ttGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCt < 1:1782713/71‑1 (MQ=255)
ttGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCt < 1:3008991/71‑1 (MQ=255)
ttGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCt < 1:3125782/71‑1 (MQ=255)
gAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCttt > 1:1568149/1‑71 (MQ=255)
gAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCtt > 1:1661466/1‑70 (MQ=255)
gAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCtt > 1:2914363/1‑70 (MQ=255)
gCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa < 1:3436204/55‑1 (MQ=255)
gCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa < 1:3167078/55‑1 (MQ=255)
gCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTa < 1:586795/55‑1 (MQ=255)
cTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGt < 1:731052/71‑1 (MQ=255)
cTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGt < 1:1212714/71‑1 (MQ=255)
cTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGt < 1:1461468/71‑1 (MQ=255)
cTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGt < 1:2964651/71‑1 (MQ=255)
cTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGt < 1:1808679/71‑1 (MQ=255)
cagcaACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGt < 1:2374377/47‑1 (MQ=255)
cagcaACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGt < 1:396927/47‑1 (MQ=255)
cagcaACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGt < 1:243205/47‑1 (MQ=255)
aaCTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGc < 1:358888/71‑1 (MQ=255)
aaCTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGc < 1:2682280/71‑1 (MQ=255)
aaCTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGc < 1:2135486/71‑1 (MQ=255)
aaCTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGc < 1:3568754/71‑1 (MQ=255)
aaCTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGc < 1:1600724/71‑1 (MQ=255)
aaCTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGc > 1:3463870/1‑71 (MQ=255)
aaCTCACGTACTTTATTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGc < 1:2374983/71‑1 (MQ=255)
aCTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGt < 1:87994/42‑1 (MQ=255)
aCGTAGTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTa < 1:2278874/70‑1 (MQ=255)
aCGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTa < 1:1081346/70‑1 (MQ=255)
aCGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTa < 1:3825101/70‑1 (MQ=255)
aCGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTAc < 1:2106427/71‑1 (MQ=255)
aCGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTAc < 1:73230/71‑1 (MQ=255)
aCGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTAc < 1:1211863/71‑1 (MQ=255)
aCGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTAc < 1:183287/71‑1 (MQ=255)
aCGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTAc < 1:2132732/71‑1 (MQ=255)
aCGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTAc < 1:2289364/71‑1 (MQ=255)
aCGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTAc < 1:3282354/71‑1 (MQ=255)
cGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTAcc < 1:3664140/71‑1 (MQ=255)
cGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTAcc < 1:3245673/71‑1 (MQ=255)
gATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGAtct < 1:2595485/68‑1 (MQ=255)
gATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGAtct < 1:3931460/68‑1 (MQ=255)
gATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGAtct < 1:54108/68‑1 (MQ=255)
aTCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTa < 1:762002/54‑1 (MQ=255)
ctctTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTAccc > 1:1784617/1‑55 (MQ=255)
ctctTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGAcc > 1:3107310/1‑60 (MQ=255)
ctctTTCGCGGCTTCCGGGTTATCTTTCAGCAAGGCAGTCGCATTCGCTTTACCCTGAcc > 1:1680828/1‑60 (MQ=255)
tctTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGt < 1:3111905/38‑1 (MQ=255)
tttCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCAcc < 1:3477776/69‑1 (MQ=255)
tttCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCAcc < 1:3429902/69‑1 (MQ=255)
tttCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCAcc < 1:2816280/69‑1 (MQ=255)
|
GCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACC > W3110S.gb/2821420‑2821540
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
