Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R2
|
356 |
55.1 |
4139924 |
95.4% |
3949487 |
63.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,125,146 |
C→T |
L11L (CTG→CTA) |
glcE ← |
glycolate oxidase FAD binding subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,125,146 | 0 | C | T | 100.0%
| 105.8
/ NA
| 34 | L11L (CTG→CTA) | glcE | glycolate oxidase FAD binding subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (24/10); total (24/10) |
TTGCTGCCCTGAATCACCAGCGGCGTTTTATCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGAAAGGTA > W3110S.gb/3125087‑3125206
|
ttGCTGCCCTGAATCACCAGCGGCGTTTTATCGCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTgg < 1:1025222/69‑1 (MQ=255)
ttGCTGCCCTGAATCACCAGCGGCGTTTTATCGCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTgg < 1:681002/69‑1 (MQ=255)
ttGCTGCCCTGAATCACCAGCGGCGTTTTATCGCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTgg < 1:2281212/69‑1 (MQ=255)
ttGCTGCCCTGAATCACCAGCGGCGTTTTATCGCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTgg < 1:2757991/69‑1 (MQ=255)
ctgcCCTGAATCACCAGCGGCGTTTTATCGCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGt < 1:79559/70‑1 (MQ=255)
ctgcCCTGAATCACCAGCGGCGTTTTATCGCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGt < 1:2150714/70‑1 (MQ=255)
cTGAATCACCAGCGGCGTTTTATCGCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGt < 1:1337382/65‑1 (MQ=255)
ggcgTTTTATCGCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGt < 1:2157407/52‑1 (MQ=255)
ttgtCGCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCa > 1:2764027/4‑64 (MQ=255)
ttATCGCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCa > 1:3192043/1‑64 (MQ=255)
ttATCGCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCa > 1:3338095/1‑64 (MQ=255)
ttATCGCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCa > 1:3351283/1‑64 (MQ=255)
ttATCGCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCa > 1:2652375/1‑64 (MQ=255)
tCGCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCAGACTCGCGTAGCATCAGAAACg < 1:1900659/70‑1 (MQ=255)
tCGCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACg < 1:619463/70‑1 (MQ=255)
gCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTcg > 1:3158727/1‑52 (MQ=255)
gCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTc > 1:3406703/1‑71 (MQ=255)
gCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTc > 1:474284/1‑71 (MQ=255)
gCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTc > 1:793714/1‑71 (MQ=255)
gCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTc > 1:3036567/1‑71 (MQ=255)
gCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTc > 1:2604519/1‑71 (MQ=255)
gCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTc > 1:1902142/1‑71 (MQ=255)
gCTAATCGCCTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTc > 1:1688919/1‑71 (MQ=255)
cTGATTCACCTGCTCCAGTAGCGCCTTGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAgg > 1:2376556/1‑71 (MQ=255)
cTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAgg > 1:2948719/1‑71 (MQ=255)
cTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAgg > 1:2781903/1‑71 (MQ=255)
cTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAgg > 1:3377952/1‑71 (MQ=255)
cTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAgg > 1:3766390/1‑71 (MQ=255)
cTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAgg > 1:4005955/1‑71 (MQ=255)
cTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAgg > 1:4102186/1‑71 (MQ=255)
cTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAgg > 1:479162/1‑71 (MQ=255)
cTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAgg > 1:1876446/1‑71 (MQ=255)
cTGATTCACCTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAgg > 1:979176/1‑71 (MQ=255)
cTGCTCCAGTAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGAAAGGTa > 1:1964379/1‑70 (MQ=255)
|
TTGCTGCCCTGAATCACCAGCGGCGTTTTATCGCTAATCGCCTGATTCACCTGCTCCAGCAGCGCCTGGCTGTAATCACACTCGCGTAGCATCAGAAACGCTCCAGTTCAGGGAAAGGTA > W3110S.gb/3125087‑3125206
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
