Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F8 I0 R2
|
296 |
77.6 |
4260404 |
88.1% |
3753415 |
61.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,249,224 |
C→T |
45.2% |
D17N (GAT→AAT) |
ruvB ← |
ATP‑dependent DNA helicase, component of RuvABC resolvasome |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,249,224 | 0 | C | T | 45.2%
| 24.9
/ 99.3
| 73 | D17N (GAT→AAT) | ruvB | ATP‑dependent DNA helicase, component of RuvABC resolvasome |
| Reads supporting (aligned to +/- strand): ref base C (26/14); new base T (12/21); total (38/35) |
| Fisher's exact test for biased strand distribution p-value = 1.93e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 |
AACGAACCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTACCTCATAACGC > minE/1249158‑1249289
|
aacgaacCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTcc > 1:1146627/1‑71 (MQ=255)
aacCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCTGCa < 1:703556/71‑1 (MQ=255)
aacCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCa < 1:830925/71‑1 (MQ=255)
acCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCa < 1:4163913/70‑1 (MQ=255)
cTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAg < 1:3009676/71‑1 (MQ=255)
cTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAg < 1:1289046/71‑1 (MQ=255)
cTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAg < 1:1592501/71‑1 (MQ=255)
cGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGt < 1:4225050/69‑1 (MQ=255)
cGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGt < 1:3170994/69‑1 (MQ=255)
cGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGt < 1:2105487/69‑1 (MQ=255)
gACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGTGGTACCg > 1:2915738/1‑71 (MQ=255)
cAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAg > 1:563035/1‑60 (MQ=255)
cAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGTGGTACCgg > 1:475323/1‑69 (MQ=255)
aaCATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGt < 1:1204116/60‑1 (MQ=255)
aaCATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCgg > 1:834757/1‑68 (MQ=255)
aaCATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCgg > 1:395449/1‑68 (MQ=255)
aaCATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCgg > 1:1249954/1‑68 (MQ=255)
aCATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAg > 1:3181956/1‑70 (MQ=255)
aCATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAg > 1:2299415/1‑70 (MQ=255)
cATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGTgg > 1:4031286/1‑60 (MQ=255)
cATACTATTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCaaa > 1:2387989/1‑56 (MQ=255)
aTACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGTGGTACCGGCAGaaa > 1:3056356/1‑71 (MQ=255)
aTACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGTGGTACCGGCAGaaa < 1:787148/71‑1 (MQ=255)
tACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGaaa > 1:981451/1‑70 (MQ=255)
tctTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGaaa > 1:3361186/1‑67 (MQ=255)
tctTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAAt > 1:1776411/1‑68 (MQ=255)
tctTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAAt > 1:2614867/1‑68 (MQ=255)
tctTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGG‑‑GAAATCAGAc > 1:3568926/1‑71 (MQ=255)
tctTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGCCAGAAAt > 1:377011/1‑68 (MQ=255)
ctTCCAGTAATTTGGGGCGAATGGCGAGATCTGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAATCAGa > 1:1558141/1‑71 (MQ=255)
ttCCAGTAATTTGGTGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGt < 1:1266732/54‑1 (MQ=255)
ttCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGt < 1:3579695/54‑1 (MQ=255)
ttCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGt < 1:3635833/54‑1 (MQ=255)
ccAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCg > 1:20616/1‑42 (MQ=255)
ccAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACgg > 1:1246490/1‑71 (MQ=255)
ccAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACgg > 1:1256308/1‑71 (MQ=255)
tAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCa < 1:578143/41‑1 (MQ=255)
tAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCa < 1:3448699/41‑1 (MQ=255)
tAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCa < 1:25568/41‑1 (MQ=255)
tAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCa < 1:401346/41‑1 (MQ=255)
tAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCa < 1:1669431/41‑1 (MQ=255)
tAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCa < 1:2436797/41‑1 (MQ=255)
tAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCa < 1:2307278/41‑1 (MQ=255)
tAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCa < 1:4113650/41‑1 (MQ=255)
aTTTGGGGCGAATGGCGCGATCTGCTACATCTTCCg > 1:321380/1‑36 (MQ=255)
aTTTGGGGCGAATGGCGCGATCTGCTACATCTTCCg > 1:1639508/1‑36 (MQ=255)
aTTTGGGGCGAATGGCGCGATCTGCTACATCTTCCg > 1:190319/1‑36 (MQ=255)
aTTTGGGGCGAATGGCGCGATCTGCTACATCTTCCg > 1:1920083/1‑36 (MQ=255)
aTTTGGGGCGAATGGCGCGATCTGCTACATCTTCCg > 1:2481764/1‑36 (MQ=255)
aTTTGGGGCGAATGGCGCGATCTGCTACATCTTCCg > 1:2774765/1‑36 (MQ=255)
tttGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGa > 1:3520759/1‑61 (MQ=255)
tttGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGa > 1:1372634/1‑61 (MQ=255)
tttGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGa > 1:2135945/1‑61 (MQ=255)
tttGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGa > 1:2501970/1‑61 (MQ=255)
ttGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCtt > 1:3395351/1‑70 (MQ=255)
cGAATGGCGCGATCTGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAatcat > 1:3579350/1‑71 (MQ=255)
cGAATGGCGCGATCTGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAatca > 1:1560675/1‑70 (MQ=255)
aTGGCGCGATCTGCTACATTTTCCGGCAAAGTGGt < 1:1040488/35‑1 (MQ=255)
aTGGCGCGATCTGCTACATTTTCCGGCAAAGTGGt < 1:954882/35‑1 (MQ=255)
aTGGCGCGATCTGCTACATTTTCCGGCAAAGTGGt < 1:959857/35‑1 (MQ=255)
aTGGCGCGATCTGCTACATTTTCCGGCAAAGTGGt < 1:1565779/35‑1 (MQ=255)
tGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGaa < 1:1396016/44‑1 (MQ=255)
tGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCtt < 1:116473/71‑1 (MQ=255)
ggCGCGATCTGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCtt > 1:4186057/1‑59 (MQ=255)
cgATCTGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTACCt > 1:954661/1‑71 (MQ=255)
tGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTACCTCa < 1:1552159/68‑1 (MQ=255)
tGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTACCTCa < 1:3931981/68‑1 (MQ=255)
tGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTACCTCa < 1:3616069/68‑1 (MQ=255)
tGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTACCTCa < 1:3104118/68‑1 (MQ=255)
tGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTACCTCa < 1:152259/68‑1 (MQ=255)
tGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTACCTCa < 1:3497568/68‑1 (MQ=255)
cTACATTTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTACCTCATAAcg > 1:2800280/1‑71 (MQ=255)
cATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTACCTCATAAcgc < 1:1568004/69‑1 (MQ=255)
|
AACGAACCTGCGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAATCAGACGGTCTGCTTCAATCATCCTTTACCTCATAACGC > minE/1249158‑1249289
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
