Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F21 I0 R2	44	10.9	588504	78.3%	460798	57.0

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,371,447	A→G	100%	F214L (TTT→CTT)	fre ←	flavin reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,371,447	0	A	G	90.1%	57.5 / ‑1.5	20	F214L (TTT→CTT)	fre	flavin reductase
Reads supporting (aligned to +/- strand): ref base A (2/0); new base G (4/14); total (6/14)
Fisher's exact test for biased strand distribution p-value = 7.89e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAACAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGG > minE/2371398‑2371485

aaCGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAACAGATCGCGGGc                            >  1:9121/1‑62 (MQ=255)

aaCGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAACAGATCGCGGGc                            >  1:476671/1‑62 (MQ=255)

                     tCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc        >  1:183684/1‑61 (MQ=255)

                     tCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACg       >  1:217150/1‑62 (MQ=255)

                     tCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACg       >  1:321592/1‑62 (MQ=255)

                     tCTTCACGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCAtc               >  1:322233/1‑54 (MQ=255)

                           cgcgCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGt      <  1:32877/57‑1 (MQ=255)

                           cgcgCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGt      <  1:452819/57‑1 (MQ=255)

                           cgcgCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGt      <  1:188715/57‑1 (MQ=255)

                           cgcgCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGt      <  1:65400/57‑1 (MQ=255)

                           cgcgCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGt      <  1:587175/57‑1 (MQ=255)

                           cgcgCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGt      <  1:63810/57‑1 (MQ=255)

                           cgcgCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTcc    <  1:79496/59‑1 (MQ=255)

                           cgcgCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCgg  <  1:93009/61‑1 (MQ=255)

                           cgcgCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCgg  <  1:201712/61‑1 (MQ=255)

                           cgcgCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCgg  <  1:122051/61‑1 (MQ=255)

                           cgcgCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCgg  <  1:547541/61‑1 (MQ=255)

                           cgcgCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCgg  <  1:359738/61‑1 (MQ=255)

                            gcgcATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGt      <  1:564794/56‑1 (MQ=255)

                            gcgcATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCgg  <  1:527454/60‑1 (MQ=255)

AACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAACAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGG > minE/2371398‑2371485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

N/A