Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F21 I0 R2
|
308 |
27.9 |
1975068 |
96.7% |
1909890 |
67.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,697,845 |
G→A |
68.9% |
G144D (GGT→GAT) |
yfhH → |
predicted DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,697,845 | 0 | G | A | 68.9%
| 55.6
/ 38.0
| 45 | G144D (GGT→GAT) | yfhH | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (7/7); new base A (19/12); total (26/19) |
| Fisher's exact test for biased strand distribution p-value = 5.28e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.25e-01 |
ACTGCATGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATG > W3110S.gb/2697777‑2697915
|
aCTGCATGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTc > 1:108563/1‑71 (MQ=255)
aCTGCATGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTc > 1:785362/1‑71 (MQ=255)
aaTGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGgcg > 1:913533/1‑71 (MQ=255)
aaTGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGAGCTTCGGATCTGGTGgcg > 1:1006101/1‑71 (MQ=255)
tctGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACt < 1:576188/58‑1 (MQ=255)
tctGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACt < 1:1712823/58‑1 (MQ=255)
tctGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACt < 1:1780398/58‑1 (MQ=255)
gcggcgGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCtgatga < 1:1506935/71‑1 (MQ=255)
gcggcgGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCtgatga < 1:1618016/71‑1 (MQ=255)
gcggcgGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCtgatga < 1:238644/71‑1 (MQ=255)
gcggcgGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCtgatga < 1:1909507/71‑1 (MQ=255)
gcggcgGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCtgatga < 1:1793271/71‑1 (MQ=255)
cggcggATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGaa < 1:1245716/71‑1 (MQ=255)
cggcggATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGaa < 1:890101/71‑1 (MQ=255)
cggcggATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGaa < 1:303239/71‑1 (MQ=255)
ggcggATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGaa < 1:522308/70‑1 (MQ=255)
gcggATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGaa < 1:175101/69‑1 (MQ=255)
cggATAATTCTGACCGGTATTTGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGTAAGCTGATGaa < 1:186974/68‑1 (MQ=255)
aTTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCg > 1:712547/1‑70 (MQ=255)
aTTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCg > 1:278398/1‑70 (MQ=255)
aTTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCg > 1:473076/1‑70 (MQ=255)
ttGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGc < 1:739835/70‑1 (MQ=255)
ttGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGc < 1:1586889/70‑1 (MQ=255)
ttGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGc < 1:156095/70‑1 (MQ=255)
ttGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGc < 1:31029/70‑1 (MQ=255)
ttGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGc < 1:566185/70‑1 (MQ=255)
ggCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAg > 1:1721913/1‑70 (MQ=255)
ggCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:711262/1‑71 (MQ=255)
ggCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:713510/1‑71 (MQ=255)
ggCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:292978/1‑71 (MQ=255)
ggCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:1779474/1‑71 (MQ=255)
ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCtt > 1:629082/1‑56 (MQ=255)
ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:1046611/1‑71 (MQ=255)
ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:536481/1‑71 (MQ=255)
ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:869427/1‑71 (MQ=255)
ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:1493772/1‑71 (MQ=255)
ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:1672273/1‑71 (MQ=255)
ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:732691/1‑71 (MQ=255)
ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:729654/1‑71 (MQ=255)
ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:1717099/1‑71 (MQ=255)
ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:1846348/1‑71 (MQ=255)
ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:704698/1‑71 (MQ=255)
ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:672152/1‑71 (MQ=255)
ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:382581/1‑71 (MQ=255)
ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGt > 1:546514/1‑71 (MQ=255)
gTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATatg < 1:623106/71‑1 (MQ=255)
gTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATatg < 1:283455/71‑1 (MQ=255)
gTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATatg < 1:786774/71‑1 (MQ=255)
gTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATatg < 1:1287653/71‑1 (MQ=255)
|
ACTGCATGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATG > W3110S.gb/2697777‑2697915
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
