Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F28 I3 R2
|
122 |
64.1 |
3058059 |
97.6% |
2984665 |
63.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
1,511,849 |
T→C |
W373R (TGG→CGG) |
pta → |
phosphate acetyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,511,849 | 0 | T | C | 98.7%
| 261.6
/ ‑6.7
| 76 | W373R (TGG→CGG) | pta | phosphate acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (37/38); minor base G (0/1); total (37/39) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTAT > minE/1511781‑1511914
|
ttCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCgg < 1:303594/71‑1 (MQ=255)
tCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGa > 1:1105170/1‑71 (MQ=255)
tCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGa > 1:2527968/1‑71 (MQ=255)
ccGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGAt > 1:2294940/1‑71 (MQ=255)
cGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGAt < 1:2926962/70‑1 (MQ=255)
cGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGAt < 1:1192489/70‑1 (MQ=255)
cGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATc > 1:2155045/1‑71 (MQ=255)
cGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATc < 1:1630592/71‑1 (MQ=255)
ggTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCg < 1:2113817/71‑1 (MQ=255)
gTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGa < 1:2406441/71‑1 (MQ=255)
ttGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGaa < 1:1920306/71‑1 (MQ=255)
aCGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAAtct < 1:2359487/71‑1 (MQ=255)
cGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGa > 1:2670294/1‑62 (MQ=255)
gATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAAtct > 1:2367926/1‑69 (MQ=255)
gATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATctct < 1:1379284/71‑1 (MQ=255)
aTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATctct > 1:2234523/1‑70 (MQ=255)
aTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTg < 1:2368266/71‑1 (MQ=255)
cACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGa < 1:1418022/70‑1 (MQ=255)
cACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGa > 1:1655540/1‑70 (MQ=255)
cACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGa > 1:2652185/1‑70 (MQ=255)
cACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGAc > 1:992724/1‑71 (MQ=255)
aCGAACGTATCGAGAAAGTTCAGGAATACGTTGCCAACTACATCAACGCTGACCGGATCGa < 1:1215893/61‑1 (MQ=255)
cGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTg < 1:2416089/71‑1 (MQ=255)
aaCGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGcc < 1:684403/71‑1 (MQ=255)
aaCGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGc > 1:45596/1‑70 (MQ=255)
aaCGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGc > 1:276335/1‑70 (MQ=255)
cGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCAc < 1:1060880/71‑1 (MQ=255)
cGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCAc < 1:2706761/71‑1 (MQ=255)
gTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATctct < 1:1714833/61‑1 (MQ=255)
tATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACtt < 1:2467426/71‑1 (MQ=255)
cGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTTCCACTTCt < 1:1581852/70‑1 (MQ=255)
gagaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTg < 1:245976/70‑1 (MQ=255)
gagaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTg < 1:2369888/70‑1 (MQ=255)
gagaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTg < 1:1546550/70‑1 (MQ=255)
gagaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTg < 1:2506898/70‑1 (MQ=255)
agaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAg < 1:2126774/71‑1 (MQ=255)
aaaGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATctct < 1:2435877/53‑1 (MQ=255)
aaaGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAgcg > 1:909939/1‑71 (MQ=255)
aaaGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAgcg < 1:3051152/71‑1 (MQ=255)
aGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAgcg > 1:2262517/1‑69 (MQ=255)
gTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACAGCCACTTCTg < 1:1013958/64‑1 (MQ=255)
cAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCcg > 1:1108384/1‑71 (MQ=255)
ggAATACGTTGCTAACTACATCAACGCTGACGGGATCGAATctct < 1:1675000/45‑1 (MQ=255)
aaTACGTTGCTAACTACATCAACGCTGACCGGATCTAATCTCTGACTGCCACTTCTGAGCGCAGCcgtcgt < 1:2306408/71‑1 (MQ=255)
aTACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCcgtcgt > 1:1731799/1‑70 (MQ=255)
aTACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCgtcgtc > 1:1961658/1‑71 (MQ=255)
tACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCa < 1:1380692/61‑1 (MQ=255)
tACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtct > 1:1668116/1‑71 (MQ=255)
tcGTTGCTAACTTCATCATCGCTGACCGGATCGAATCTCTGACTGCCACTTCt < 1:730435/52‑1 (MQ=255)
cGTTGCTAACTACATCAACGCTGACCGGATCGAAt > 1:1876984/1‑35 (MQ=255)
cGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctgt > 1:868738/1‑71 (MQ=255)
cGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctgt < 1:2866054/71‑1 (MQ=255)
cGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctg > 1:2087752/1‑70 (MQ=255)
tGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGa > 1:1323059/1‑51 (MQ=255)
tGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGtctc > 1:338600/1‑71 (MQ=255)
gCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGtctc < 1:29414/70‑1 (MQ=255)
cTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCg > 1:855685/1‑71 (MQ=255)
tAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAgcgc < 1:2368132/52‑1 (MQ=255)
aCTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAgcgc > 1:1892823/1‑50 (MQ=255)
aCTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAgcg > 1:2661452/1‑49 (MQ=255)
aCTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCgtcgtc > 1:702959/1‑60 (MQ=255)
aCTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGc < 1:534550/69‑1 (MQ=255)
aCTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGc < 1:253206/69‑1 (MQ=255)
cTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGcc > 1:1080964/1‑69 (MQ=255)
cTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTg < 1:2215978/71‑1 (MQ=255)
cATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCg < 1:757034/70‑1 (MQ=255)
cATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGt > 1:1882942/1‑71 (MQ=255)
cATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGt > 1:270265/1‑71 (MQ=255)
tCAACGCTGACCGGATCGAATCTCTGACTGCCACtt > 1:1572362/1‑36 (MQ=255)
tCAACGCTGACCGGATCGAATCTCTGACTGCCACtt > 1:1829264/1‑36 (MQ=255)
tCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCcgtcgt > 1:75838/1‑53 (MQ=255)
cAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTcc > 1:2501760/1‑71 (MQ=255)
aCGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGt < 1:905532/71‑1 (MQ=255)
gCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTa > 1:2079845/1‑71 (MQ=255)
gACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTAt > 1:203513/1‑69 (MQ=255)
gACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTAt > 1:2030872/1‑69 (MQ=255)
|
TTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTAT > minE/1511781‑1511914
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
