Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F28 I3 R2
|
122 |
64.1 |
3058059 |
97.6% |
2984665 |
63.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
1,647,049 |
C→T |
Q102* (CAA→TAA) |
bcp → |
thiol peroxidase, thioredoxin‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,647,049 | 0 | C | T | 100.0%
| 113.3
/ NA
| 38 | Q102* (CAA→TAA) | bcp | thiol peroxidase, thioredoxin‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (20/18); total (20/18) |
GTTTTGCGGAAAAAGAGCTGCTTAACTTTACGCTCCTGTCTGATGAGGACCACCAGGTGTGCGAACAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCATTCATCGCATCAGCT > minE/1646984‑1647115
|
gTTTTGCGGAAAAAGAGCTGCTTAACTTTACGCTCCTGTCTGATGAGGACCACCAGGTGTGCGAATAATTc < 1:892884/71‑1 (MQ=255)
gTTTTGCGGAAAAAGAGCTGCTTAACTTTACGCTCCTGTCTGATGAGGACCACCAGGTGTGCGAATAATTc > 1:2657375/1‑71 (MQ=255)
ttGCGGAAAAAGAGCTGCTTAACTTTACGCTCCTGTCTGATGAGGACCACCAGGTGTGCGAATAATTCGGc > 1:1903205/1‑71 (MQ=255)
cGGAAAAAGAGCTGCTTAACTTTACGCTCCTGTCTGATGAGGACCACCAGGTGTGCGAATAATTCGGCGt < 1:1078555/70‑1 (MQ=255)
gAAAAAGAGCTGCTTAACTTTACGCTCCTGTCTGATGAGGACCACCAGGTGTGCGAATAATTCGGCGTCTg < 1:2626155/71‑1 (MQ=255)
aaaGAGCTGCTTAACTTTACGCTCCTGTCTGATGAGGACCACCAGGTGTGCGAATAATTCGGCGTCTgggg > 1:1855256/1‑71 (MQ=255)
agagCTGCTTAACTTTACGCTCCTGTCTGATGAGGACCACCAGGTGTGCGAATAATTCGGCGTCTGGGGt > 1:2331874/1‑70 (MQ=255)
agCTGCTTAACTTTACGCTCCTGTCTGATGAGGACCACCAGGTGTGCGAATAATTCGGCGTCTGGGGTGaa < 1:241193/71‑1 (MQ=255)
agCTGCTTAACTTTACGCTCCTGTCTGATGAGGACCACCAGGTGTGCGAATAATTCGGCGTCTGGGGTGa > 1:2672433/1‑70 (MQ=255)
ctTAACTTTACGCTCCTGTCTGATGAGGACCACCAGGTGTGCTAATAATTCGGCGTCTGGGGTGAAAAg < 1:2479346/69‑1 (MQ=255)
ctTAACTTTACGCTCCTGTCTGATGAGGACCACCAGGTGTGCGAATAATTCGGCGTCTGGGGTGAAAAg < 1:37350/69‑1 (MQ=255)
ctTAACTTTACGCTCCTGTCTGATGAGGACCACCAGGTGTGCGAATAATTCGGCGTCTGGGGTGAAAAGt > 1:1145955/1‑70 (MQ=255)
aaCTTTACGCTCCTGTCTGATGAGGACCACCAGGTGTGCGAATAATTCGGCGTCTGGGGt > 1:733981/1‑60 (MQ=255)
aaCTTTACGCTCCTGTCTGATGAGGACCACCAGGTGTGCGAATAATTCGGCGTCTGGGGTGAAAAGTcc < 1:537659/69‑1 (MQ=255)
tACGCTCCTGTCTGATGAGGACCACCAGGTGTGCGAATAATTCGGCGTCTGGGGTGAAAAGTCCTTCAt < 1:89684/69‑1 (MQ=255)
tACGCTCCTGTCTGATGAGGACCACCAGGTGTGCGAATAATTCGGCGTCTGGGGTGAAAAGTCCTTCATgg > 1:2064129/1‑71 (MQ=255)
tACGCTCCTGTCTGATGAGGACCACCAGGTGTGCGAATAATTCGGCGTCTGGGGTGAAAAGTCCTTCATg < 1:1181508/70‑1 (MQ=255)
gCTCCTGTCTGATGAGGACCACCAGGTGTGCGAATAATTCGGCGTCTGGGGTg < 1:1725937/53‑1 (MQ=255)
cTCCTGTCTGATGAGGACCACCAGGTGTGCGAATAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCaa < 1:1425411/71‑1 (MQ=255)
tCCTGTCTGATGAGGACCACCAGGTGTGCGAATAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCaa > 1:1137242/1‑70 (MQ=255)
ccTGTCTGATGAGGACCACCAGGTGTGCGAATAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCaaaa > 1:3019818/1‑71 (MQ=255)
cTGTCTGATGAGGACCACCAGGTGTGCGAATAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCaaa < 1:1101752/69‑1 (MQ=255)
tCTGATGAGGACCACCAGGTGTGCGAATAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCaa < 1:27570/65‑1 (MQ=255)
tgaGGACCACCAGGTGTGCGAATAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATg > 1:1075964/1‑71 (MQ=255)
gaGGACCACCAGGTGTGCGAATAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATgg > 1:743449/1‑71 (MQ=255)
aGGACCACCAGGTGTGCGAATAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATgg < 1:2635074/70‑1 (MQ=255)
gACCACCAGGTGTGCGAATAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCAt < 1:1751890/71‑1 (MQ=255)
gACCACCAGGTGTGCGAATAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCAt > 1:380895/1‑71 (MQ=255)
accaccAGGTGTGCGAATAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCAtt > 1:2270130/1‑71 (MQ=255)
ccaGGTGTGCGAATAATACGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCATTCATc > 1:1233984/1‑71 (MQ=255)
aGGTGTGCGAATAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCATTCATCg > 1:1670893/1‑70 (MQ=255)
ggTGTGCGAATAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCATTCATCGCa < 1:1116017/71‑1 (MQ=255)
tgCGAATAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCATTCATCGCATCa < 1:1372171/70‑1 (MQ=255)
cGAATAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCt > 1:333982/1‑47 (MQ=255)
cGAATAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTAc > 1:1850844/1‑49 (MQ=255)
cGAATAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTAc > 1:1563854/1‑49 (MQ=255)
cGAATAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCATTCATCGCATCAGc < 1:2251254/70‑1 (MQ=255)
cGAATAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCATTCATCGCATCAGCt > 1:1043142/1‑71 (MQ=255)
|
GTTTTGCGGAAAAAGAGCTGCTTAACTTTACGCTCCTGTCTGATGAGGACCACCAGGTGTGCGAACAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCATTCATCGCATCAGCT > minE/1646984‑1647115
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
