Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,013,795 |
A→G |
S39G (AGC→GGC) |
pqiB → |
paraquat‑inducible protein B |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,013,795 | 0 | A | G | 100.0%
| 149.0
/ NA
| 43 | S39G (AGC→GGC) | pqiB | paraquat‑inducible protein B |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (16/27); total (16/27) |
GTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACAGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCGGAAGGAATTGAAGGTGGCAAAACC > W3110S.gb/1013728‑1013860
|
gTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGc > 1:2035225/1‑70 (MQ=255)
ccGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCAt < 1:2588986/68‑1 (MQ=255)
ccGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAg < 1:1472632/71‑1 (MQ=255)
ccGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAg > 1:2913395/1‑71 (MQ=255)
cGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGATTCTTTTTTATCATTACGGCCATCAgg < 1:2810755/71‑1 (MQ=255)
gtgGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGa > 1:2802217/1‑70 (MQ=255)
gATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCa > 1:16829/1‑63 (MQ=255)
atatTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGa < 1:601446/71‑1 (MQ=255)
atatTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGa < 1:3807962/71‑1 (MQ=255)
atatTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGa < 1:3262985/71‑1 (MQ=255)
atatTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGa < 1:2895327/71‑1 (MQ=255)
tCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGtt > 1:3364288/1‑69 (MQ=255)
tCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGa > 1:1127452/1‑71 (MQ=255)
tCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGa > 1:4219394/1‑71 (MQ=255)
tCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTg < 1:3418492/71‑1 (MQ=255)
gTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGaa > 1:3234524/1‑60 (MQ=255)
gTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGAt > 1:2162972/1‑71 (MQ=255)
gcgcTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCa > 1:550400/1‑67 (MQ=255)
cgcTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACcgcg < 1:3911686/71‑1 (MQ=255)
cgcTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACcgcg < 1:1479598/71‑1 (MQ=255)
gcTCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACcgcg < 1:3512568/70‑1 (MQ=255)
tCATTGGGGCCTGGGTTCTTTTTTATCATTACGGCCAt < 1:4304679/38‑1 (MQ=255)
ggggCCTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCgg > 1:3524881/1‑70 (MQ=255)
cTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCggaagg < 1:3303291/69‑1 (MQ=255)
cTGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCggaagg < 1:2939022/69‑1 (MQ=255)
tGGGTTCTTTTTTATCATTACGGCCATCAGGGACCGAAAGTGACCCt < 1:2713954/47‑1 (MQ=38)
ggTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCGGAAGGAATTg < 1:2188591/71‑1 (MQ=255)
gTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACcgcg < 1:3779661/54‑1 (MQ=255)
gTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACcgcg < 1:3920851/54‑1 (MQ=255)
gTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACcgcg < 1:2754328/54‑1 (MQ=255)
gTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCggaagga < 1:1798933/66‑1 (MQ=255)
gTTCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCggaagga < 1:1158679/66‑1 (MQ=255)
ttCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCGGAAGGAATTg > 1:2545730/1‑69 (MQ=255)
ttCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCGGAAGGAATTGaa < 1:1025445/71‑1 (MQ=255)
ttCTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCGGAAGGAATTGaa < 1:2880746/71‑1 (MQ=255)
cTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGaa > 1:191178/1‑53 (MQ=255)
cTTTTTTATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCGGAAGCAATTGAAgg > 1:4500018/1‑71 (MQ=255)
ttttATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCGGAAGGAATTGa > 1:3160840/1‑65 (MQ=255)
ttttATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCGGAAGGAATTGAAgg < 1:4335292/68‑1 (MQ=255)
tttATCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCGGAAGGAATTGAAGGTgg > 1:2897568/1‑70 (MQ=255)
tCATTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCGGAAGGAATTGAAGGTGGCaa < 1:2924727/69‑1 (MQ=255)
cATTACGGCGATCAGGGACCGGAAGTGACCCTGATGACCGCGAATGCGGAAGGAATTGaa < 1:1478972/60‑1 (MQ=255)
aTTACGGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCGGAAGGAATTGAAGGTGGCAAAacc < 1:1414372/71‑1 (MQ=255)
|
GTCTCCCGTGTGGATATTTCCTATCGTCACGGCGCTCATTGGGGCCTGGGTTCTTTTTTATCATTACAGCCATCAGGGACCGGAAGTGACCCTGATCACCGCGAATGCGGAAGGAATTGAAGGTGGCAAAACC > W3110S.gb/1013728‑1013860
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
