Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,057,926 |
G→A |
R151W (CGG→TGG) |
torR ← |
DNA‑binding response regulator in two‑component regulatory system with TorS |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,057,926 | 0 | G | A | 100.0%
| 135.3
/ NA
| 45 | R151W (CGG→TGG) | torR | DNA‑binding response regulator in two‑component regulatory system with TorS |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (15/30); total (15/30) |
CGGATTCGTCACAAATGCCACCAACATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCGCTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGCGATAGCAGTTGTCCTGA > W3110S.gb/1057858‑1057987
|
cGGATTCGTCACAAATGCCACCAACATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACt > 1:1060777/1‑71 (MQ=255)
ttCGTCACAAATGCCACCAACATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAg > 1:881375/1‑71 (MQ=255)
cGTCACAAATGCCACCAACATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCg > 1:1618208/1‑71 (MQ=255)
cGTCACAAATGCCACCAACATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCg > 1:2490720/1‑71 (MQ=255)
gTCACAAATGCCACCAACATTTCATACTCTGCGCTGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCg < 1:2261180/70‑1 (MQ=255)
gTCACAAATGCCACCAACATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCg < 1:1242320/70‑1 (MQ=255)
gTCACAAATGCCACCAACATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCg < 1:847329/70‑1 (MQ=255)
gTCACAAATGCCACCAACATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCg < 1:1658383/70‑1 (MQ=255)
gTCACAAATGCCACCAACATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCg < 1:3015046/70‑1 (MQ=255)
aaTGCCACCAACATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGgcg > 1:4074413/1‑71 (MQ=255)
ccaACATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCg < 1:3002119/57‑1 (MQ=255)
aaCATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTa > 1:330548/1‑57 (MQ=255)
aaCATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTa > 1:2514920/1‑57 (MQ=255)
aaCATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTa > 1:3061964/1‑57 (MQ=255)
aaCATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACAtt > 1:2164794/1‑71 (MQ=255)
cATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACAtt > 1:1100729/1‑69 (MQ=255)
aTTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAg > 1:293705/1‑71 (MQ=255)
tCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGc < 1:244080/70‑1 (MQ=255)
tCATACTCTGCGCGGGTCAGTTTAATCGGCGCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGc < 1:285109/70‑1 (MQ=255)
aTACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAAt < 1:2638140/71‑1 (MQ=255)
tACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATa < 1:2349213/71‑1 (MQ=255)
ctctGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATaa < 1:533304/70‑1 (MQ=255)
tctGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGc < 1:384075/64‑1 (MQ=255)
tctGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAAcc > 1:1691027/1‑71 (MQ=255)
tctGCGCGGGGCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGc < 1:554858/64‑1 (MQ=39)
tGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCaa > 1:842915/1‑64 (MQ=255)
tGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCgg > 1:173257/1‑71 (MQ=255)
tGCGCGGGTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCgg > 1:4221204/1‑71 (MQ=255)
ggTCAGTTTAATCGGCTCGCCATCCCACTCCAGCg < 1:3217220/35‑1 (MQ=255)
ggTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGc < 1:179666/71‑1 (MQ=255)
gTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGc < 1:4593901/70‑1 (MQ=255)
gTCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGCg < 1:4049125/71‑1 (MQ=255)
tCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGCGa < 1:3381062/71‑1 (MQ=255)
tCAGTTTAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGCGa < 1:3922308/71‑1 (MQ=255)
tttAATCGGCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGCGATAGc < 1:1586874/71‑1 (MQ=255)
ggCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGCGATAGCAGTTGt < 1:3186127/70‑1 (MQ=255)
ggCTCGCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGCGATAGCAGTTGt < 1:2056027/70‑1 (MQ=255)
gCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCTGCAAAGCGATAGCAGTTGTCCTg < 1:40680/69‑1 (MQ=255)
gCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGCGATAGCAGTTGTCCTg < 1:4592674/69‑1 (MQ=255)
gCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGCGATAGCAGTTGTCCTg < 1:4624562/69‑1 (MQ=255)
gCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGCGATAGCAGTTGTCCTg < 1:2935088/69‑1 (MQ=255)
gCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGCGATAGCAGTTGTCCTg < 1:2287037/69‑1 (MQ=255)
gCCATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGCGATAGCAGTTGTCCTGa < 1:4351671/70‑1 (MQ=255)
ccATCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGCGATAGCAGTTGTCCTg < 1:1732897/68‑1 (MQ=255)
tCCCACTCCAGCGTATGGCGCGACACATTCAGGCAATa < 1:4278171/38‑1 (MQ=255)
|
CGGATTCGTCACAAATGCCACCAACATTTCATACTCTGCGCGGGTCAGTTTAATCGGCTCGCCATCCCGCTCCAGCGTATGGCGCGACACATTCAGGCAATAACCGGCAAAGCGATAGCAGTTGTCCTGA > W3110S.gb/1057858‑1057987
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
