Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,482,053 |
A→G |
D401G (GAT→GGT) |
ynbC → |
predicted hydrolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,482,053 | 0 | A | G | 100.0%
| 143.3
/ NA
| 42 | D401G (GAT→GGT) | ynbC | predicted hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (19/23); total (19/23) |
TGTTGGCTGGCGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAGGGCATGGGCGCTATGTACTG > W3110S.gb/1481985‑1482120
|
tgtTGGCTGGCGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGtc > 1:3882302/1‑71 (MQ=255)
tgtTGGCTGGCGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGtc > 1:1761978/1‑71 (MQ=255)
ttGGCTGGCGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGtctc < 1:305712/71‑1 (MQ=255)
ttGGCTGGCGCGGTATTCGCCAGCCCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGtctc > 1:719693/1‑71 (MQ=255)
tggctggcGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGtctc < 1:1874006/70‑1 (MQ=255)
ggctggcGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTcc < 1:1103031/70‑1 (MQ=255)
ggctggcGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCa > 1:1968179/1‑71 (MQ=255)
ggTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAgg < 1:3586554/71‑1 (MQ=255)
ggTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAgg < 1:1723460/71‑1 (MQ=255)
gTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAgg < 1:1766271/70‑1 (MQ=255)
gTATTCGCCAGCGAAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGt < 1:298851/71‑1 (MQ=255)
ttCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGttt < 1:33109/70‑1 (MQ=255)
cGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGc > 1:1700244/1‑71 (MQ=255)
cGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGc > 1:818457/1‑71 (MQ=255)
cGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGc > 1:2332008/1‑71 (MQ=255)
cGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGc > 1:1371093/1‑71 (MQ=255)
gcAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCcgc > 1:1258604/1‑71 (MQ=255)
aaaCCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTg < 1:103372/71‑1 (MQ=255)
cATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGa < 1:2683385/71‑1 (MQ=255)
cATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGa < 1:2805666/71‑1 (MQ=255)
cATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGa < 1:329035/71‑1 (MQ=255)
cATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGa < 1:1573167/71‑1 (MQ=255)
aTTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGAc < 1:161675/71‑1 (MQ=255)
tACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACa > 1:206554/1‑69 (MQ=255)
tACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACa > 1:2993540/1‑69 (MQ=255)
tACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACAtt > 1:958889/1‑71 (MQ=255)
tACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACAtt > 1:426055/1‑71 (MQ=255)
tACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACAtt > 1:3463456/1‑71 (MQ=255)
tACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACAtt > 1:413168/1‑71 (MQ=255)
tACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACAtt > 1:3825863/1‑71 (MQ=255)
aCAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTg > 1:2457379/1‑71 (MQ=255)
aaTACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGa < 1:2932974/63‑1 (MQ=255)
cTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAgg > 1:152509/1‑71 (MQ=255)
aTTAAACAAGCCGTTGCCGGTCTCCATGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAggg < 1:1586801/69‑1 (MQ=255)
aTTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAGGGc < 1:2868740/70‑1 (MQ=255)
tAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAGGGCATg > 1:4673894/1‑71 (MQ=255)
aaGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAGGGCATGGgcgc < 1:3271242/71‑1 (MQ=255)
gCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAGGGCATGGGCGCTa < 1:3751755/71‑1 (MQ=255)
cGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAGGGCATGTGCGCTATg < 1:535855/71‑1 (MQ=255)
cGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAGGGCATGGGCGCTATg < 1:3499953/71‑1 (MQ=255)
cGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAGGGCATGGGCGCTATg < 1:4214294/71‑1 (MQ=255)
ccGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAGGGCATGGGCGCTATGTACTg < 1:862853/71‑1 (MQ=255)
|
TGTTGGCTGGCGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAGGGCATGGGCGCTATGTACTG > W3110S.gb/1481985‑1482120
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
