Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,020,305 |
A→G |
T75A (ACG→GCG) |
fliJ → |
flagellar protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,020,305 | 0 | A | G | 100.0%
| 139.8
/ NA
| 41 | T75A (ACG→GCG) | fliJ | flagellar protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/21); total (20/21) |
ACCTCAACAGCGATATGAGTGCCGGGATAACCAGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACA > W3110S.gb/2020237‑2020368
|
aCCTCAACAGCGATATGAGTGCCGGGATAACCAGCAACCGCTGGATCAACTATCGGCAGTTTATCCAGGCg > 1:2049228/1‑71 (MQ=255)
aCCTCAACAGCGATATGAGTGCCGGGATAACCAGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGGCg > 1:950825/1‑71 (MQ=255)
aCCTCAACAGCGATATGAGTGCCGGGATAACCAGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGGCg > 1:1362734/1‑71 (MQ=255)
aCCTCAACAGCGATATGAGTGCCGGGATAACCAGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGGCg > 1:3687480/1‑71 (MQ=255)
aGCGATATGAGTGCCGGGATAACCAGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGGCGCTGGaaaa < 1:81931/71‑1 (MQ=255)
gATATGAGTGCCGGGATAACCAGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGGCGCTGGAAAAAGc < 1:3571800/71‑1 (MQ=255)
tGAGTGCCGTGATAACCAGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCAt < 1:3600472/70‑1 (MQ=255)
tGAGTGCCGGGATAACCAGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCAt < 1:3539901/70‑1 (MQ=255)
tGAGTGCCGGGATAACCAGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCAt < 1:4481955/70‑1 (MQ=255)
aGTGCCGGGATAACCAGCAACCGCTGGATCAACTATCAGCAGTTTATCAAGGCGCTGGAAAAAGCCATTAc > 1:501314/1‑71 (MQ=255)
aTAACCAGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCg > 1:3518535/1‑71 (MQ=255)
tAACCAGCAACCGCTGGATCAACTATCAGCAGTTTTTCCAGGCGCTGGAGAAAGCCATTACTCAGCATCGc < 1:3838614/71‑1 (MQ=255)
aCCAGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGc > 1:425391/1‑69 (MQ=255)
aCCAGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGc > 1:1633899/1‑69 (MQ=255)
aCCAGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGc > 1:1423032/1‑69 (MQ=255)
aGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCa < 1:3999292/71‑1 (MQ=255)
aGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCa < 1:1571137/71‑1 (MQ=255)
gCTGGATCAACTATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAAt < 1:2193497/71‑1 (MQ=255)
cTGGATCAACTATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATc < 1:1555884/71‑1 (MQ=255)
cTGGATCAACTATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATc < 1:3167968/71‑1 (MQ=255)
gATCAACTATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCATTACTCAg < 1:3910383/49‑1 (MQ=255)
gATCAACTATCAGAAGTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAg < 1:3628409/70‑1 (MQ=255)
gATCAACCATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCa < 1:287182/69‑1 (MQ=255)
tATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCa < 1:3370237/53‑1 (MQ=255)
tATCAGCAGTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACg > 1:1412777/1‑69 (MQ=255)
aTCAGCAGTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCa > 1:1820272/1‑43 (MQ=255)
agcagTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGaa < 1:3812831/70‑1 (MQ=255)
gTTTATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGtt > 1:2649869/1‑70 (MQ=255)
tttATCCAGGCGCTGGAAAAAGCCATTACTGAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGa < 1:1756976/71‑1 (MQ=255)
tttATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATc < 1:2570673/52‑1 (MQ=255)
tttATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGa < 1:4095117/71‑1 (MQ=255)
tttATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGa < 1:1648424/71‑1 (MQ=255)
ttATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGa > 1:2566092/1‑70 (MQ=255)
tATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTaa > 1:3596533/1‑48 (MQ=255)
tATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTaa > 1:3232322/1‑48 (MQ=255)
tATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGAc < 1:3932769/70‑1 (MQ=255)
tATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACa > 1:2856976/1‑71 (MQ=255)
tATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACa > 1:2879679/1‑71 (MQ=255)
tATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACa > 1:4534380/1‑71 (MQ=255)
tATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACa > 1:890633/1‑71 (MQ=255)
tATCCAGGCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACa > 1:3291792/1‑71 (MQ=255)
|
ACCTCAACAGCGATATGAGTGCCGGGATAACCAGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACA > W3110S.gb/2020237‑2020368
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
