Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,204,561 |
G→A |
D317N (GAT→AAT) |
yehI → |
conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,204,561 | 0 | G | A | 100.0%
| 140.2
/ NA
| 48 | D317N (GAT→AAT) | yehI | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/27); total (21/27) |
TGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCA > W3110S.gb/2204498‑2204616
|
tGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATaaaaa > 1:1191230/1‑71 (MQ=255)
gCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAaaacaaa < 1:695309/71‑1 (MQ=255)
gCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAaaacaaa < 1:4566983/71‑1 (MQ=255)
gTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAaaaca < 1:4364862/66‑1 (MQ=255)
gTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAaaaca < 1:270930/66‑1 (MQ=255)
tCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCa < 1:4135132/71‑1 (MQ=255)
ctgctgGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAg > 1:3675089/1‑71 (MQ=255)
ctgctgGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAg > 1:4598939/1‑71 (MQ=255)
ctgctgGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAg > 1:1926963/1‑71 (MQ=255)
ctgctgGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAg > 1:537486/1‑71 (MQ=255)
tgctgGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGc > 1:1090551/1‑71 (MQ=255)
gTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGc < 1:2310311/70‑1 (MQ=255)
aaTTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACgtg > 1:491420/1‑71 (MQ=255)
aTTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACgtgt < 1:2750424/71‑1 (MQ=255)
aTTAACCATCCGCAAGTCATCCGAACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACgtgt < 1:4260772/71‑1 (MQ=255)
ttAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACgtg > 1:3031209/1‑69 (MQ=255)
ttAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACgtg > 1:3075084/1‑69 (MQ=255)
ccATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTa < 1:3643191/71‑1 (MQ=255)
ccATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTa < 1:1705271/71‑1 (MQ=255)
ccATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTa < 1:131203/71‑1 (MQ=255)
cATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTa < 1:2491286/70‑1 (MQ=255)
ccGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATa > 1:1211650/1‑71 (MQ=255)
cGCAAGTCATCCGCACTTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATAc > 1:3856161/1‑71 (MQ=255)
cGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATAc > 1:3497923/1‑71 (MQ=255)
cGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATAc < 1:4072608/71‑1 (MQ=255)
cGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATAc > 1:2809145/1‑71 (MQ=255)
cGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATAc < 1:2485467/71‑1 (MQ=255)
cGCAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATAc > 1:503103/1‑71 (MQ=255)
cAAGTCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACgtg < 1:4687295/58‑1 (MQ=255)
tCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATaaa < 1:3067467/71‑1 (MQ=255)
tCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATaaa < 1:455195/71‑1 (MQ=255)
tCATCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACACAGCCTGCAACGTGTAGCTAAATACCATaaa < 1:1479773/71‑1 (MQ=255)
aTCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATaaaa < 1:2481964/70‑1 (MQ=255)
tCCGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCAt < 1:2048958/65‑1 (MQ=255)
cGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAg > 1:881879/1‑52 (MQ=255)
cGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATa > 1:3452613/1‑59 (MQ=255)
cGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATaa > 1:3038261/1‑65 (MQ=255)
cGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACtt > 1:2774476/1‑71 (MQ=255)
cGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACtt > 1:1458973/1‑71 (MQ=255)
cGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACtt > 1:1123862/1‑71 (MQ=255)
cGCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACt < 1:2688687/70‑1 (MQ=255)
gCACCTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTc < 1:4149929/71‑1 (MQ=255)
ccTTGCTACTGGTGGCTAATAAAAACAAACCCATCCTGCAACGTGTAGCTAAATACCATAAAAACTTcccc < 1:1437889/71‑1 (MQ=255)
ccTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTcccc < 1:3415391/71‑1 (MQ=255)
ccTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTcccc < 1:198939/71‑1 (MQ=255)
ccTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTcccc < 1:1543100/71‑1 (MQ=255)
cTTGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTa < 1:1975717/50‑1 (MQ=255)
ttGCTACTGGTGGCTAATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCa > 1:2232860/1‑71 (MQ=255)
|
TGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCA > W3110S.gb/2204498‑2204616
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
