Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,416,039 |
G→A |
R254C (CGT→TGT) |
yfbS ← |
predicted transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,416,039 | 0 | G | A | 100.0%
| 198.2
/ NA
| 66 | R254C (CGT→TGT) | yfbS | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (31/35); total (31/35) |
AACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGAC > W3110S.gb/2415974‑2416104
|
aaCGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCAc > 1:4138784/1‑71 (MQ=255)
aaCGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCAc > 1:3291773/1‑71 (MQ=255)
aaCGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCAc > 1:3413788/1‑71 (MQ=255)
aaCGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCAc > 1:4562970/1‑71 (MQ=255)
aaCGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCAc > 1:3699931/1‑71 (MQ=255)
aaCGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCAc > 1:2122311/1‑71 (MQ=255)
cACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCTATGACGTTAGCGCCATAACACTCACGTAAtt > 1:1985570/1‑71 (MQ=255)
cACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAAtt > 1:1476351/1‑71 (MQ=255)
cACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAAtt > 1:601735/1‑71 (MQ=255)
cACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAAtt > 1:181121/1‑71 (MQ=255)
cACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAAtt > 1:1691002/1‑71 (MQ=255)
gATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTga < 1:2540016/71‑1 (MQ=255)
tAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTgag < 1:1286014/70‑1 (MQ=255)
aaCGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAAttt > 1:1271025/1‑66 (MQ=255)
aaCGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAAttt > 1:4504179/1‑66 (MQ=255)
aaCGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAAttt > 1:3471654/1‑66 (MQ=255)
aCGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGat < 1:4176172/70‑1 (MQ=255)
cgcgACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGatca < 1:3105887/71‑1 (MQ=255)
cgacgaAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCTCCATAACACTCACGTAATTTGAGatcatc < 1:441381/71‑1 (MQ=255)
cgacgaAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGatcatc < 1:4474052/71‑1 (MQ=255)
acgaAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCa < 1:2289601/71‑1 (MQ=255)
aaaaCGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGcc < 1:4560930/71‑1 (MQ=255)
aaaaCGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGcc < 1:1393670/71‑1 (MQ=255)
gccAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCa < 1:65751/71‑1 (MQ=255)
cAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAAt < 1:1386532/71‑1 (MQ=255)
cAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAAt < 1:3604258/71‑1 (MQ=255)
cAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAAt < 1:639554/71‑1 (MQ=255)
cAGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAAt < 1:1871694/71‑1 (MQ=255)
aGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAAt > 1:938742/1‑70 (MQ=255)
aGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAAt > 1:3673247/1‑70 (MQ=255)
aGCGTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCACCCAGCCGTTGACCAAt > 1:172109/1‑70 (MQ=255)
gTTCAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCAtt < 1:1683185/71‑1 (MQ=255)
cAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCaa > 1:4673669/1‑63 (MQ=255)
cAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCaa > 1:858149/1‑63 (MQ=255)
cAACACCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGc > 1:1171316/1‑71 (MQ=255)
acacCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGa < 1:3404643/71‑1 (MQ=255)
acagATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGAt > 1:18221/4‑70 (MQ=255)
acCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATc > 1:2307719/1‑71 (MQ=255)
acCGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATc > 1:230622/1‑71 (MQ=255)
ccGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATcc > 1:3430456/1‑71 (MQ=255)
ccGATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATcc > 1:2482209/1‑71 (MQ=255)
gATGACGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCgg > 1:1630447/1‑71 (MQ=255)
aCGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCg < 1:2738950/71‑1 (MQ=255)
aCGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCg < 1:3689952/71‑1 (MQ=255)
aCGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCg < 1:3696393/71‑1 (MQ=255)
aCGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCg < 1:2236187/71‑1 (MQ=255)
cGTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCg < 1:3248351/70‑1 (MQ=255)
gTTAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCg < 1:2104882/69‑1 (MQ=255)
ttAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCa < 1:1220300/52‑1 (MQ=255)
ttAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCa < 1:1959018/52‑1 (MQ=255)
ttAGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCg < 1:2602981/68‑1 (MQ=255)
ttAGCGCCATAACACTCACCTAATTTGAGATCATCCAGCCGTTGACCAATCa < 1:549864/52‑1 (MQ=255)
aGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAg > 1:1587019/1‑71 (MQ=255)
aGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAg > 1:3526051/1‑71 (MQ=255)
aGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAg > 1:2794009/1‑71 (MQ=255)
aGCGCCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAg > 1:2664060/1‑71 (MQ=255)
gcgcCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGc < 1:4093483/71‑1 (MQ=255)
gcCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGAcc < 1:3708806/42‑1 (MQ=255)
gcCATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCa < 1:2555918/71‑1 (MQ=255)
ccATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGc < 1:2540930/68‑1 (MQ=255)
ccATAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCa < 1:166500/70‑1 (MQ=255)
aTAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGAc < 1:982353/71‑1 (MQ=255)
aTAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGAc < 1:3999634/71‑1 (MQ=255)
tAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCa < 1:640192/67‑1 (MQ=255)
tAACACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCa < 1:370716/67‑1 (MQ=255)
aaCACTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGaa > 1:3868210/1‑60 (MQ=255)
|
AACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGAC > W3110S.gb/2415974‑2416104
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
