Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,585,491 |
T→C |
L369L (TTG→CTG) |
narQ → |
sensory histidine kinase in two‑component regulatory system with NarP (NarL) |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,585,491 | 0 | T | C | 100.0%
| 222.7
/ NA
| 65 | L369L (TTG→CTG) | narQ | sensory histidine kinase in two‑component regulatory system with NarP (NarL) |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (36/29); total (36/29) |
TTAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGAT > W3110S.gb/2585423‑2585558
|
ttAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTg > 1:3387289/1‑71 (MQ=255)
tAATCAGGTGCAGAAGCATTTTCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTg > 1:3724631/1‑70 (MQ=255)
tAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTg > 1:1970722/1‑70 (MQ=255)
gCATTTTCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTc < 1:3470479/71‑1 (MQ=255)
ttttCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAgg < 1:12877/71‑1 (MQ=255)
ttCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTg > 1:1241982/1‑50 (MQ=255)
ttCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGc > 1:352520/1‑51 (MQ=255)
ttCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGc > 1:4092146/1‑64 (MQ=255)
ttCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGt > 1:4387228/1‑70 (MQ=255)
ttCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGt > 1:589192/1‑70 (MQ=255)
ttCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTa > 1:1817820/1‑71 (MQ=255)
ttCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTa > 1:402344/1‑71 (MQ=255)
ttCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTa > 1:382346/1‑71 (MQ=255)
ttCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTa > 1:1980648/1‑71 (MQ=255)
aaTTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTct < 1:2045642/71‑1 (MQ=255)
attattGTTGATGGAAGAACGGGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTc < 1:2346396/59‑1 (MQ=38)
tattGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCa > 1:878976/1‑43 (MQ=255)
tattGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAg > 1:1729243/1‑59 (MQ=255)
tattGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTcttac > 1:4307730/1‑71 (MQ=255)
tattGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTcttac > 1:576645/1‑71 (MQ=255)
tattGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTcttac > 1:1337492/1‑71 (MQ=255)
tattGTTGATGGAAGAACGTGCGACAATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTcttac > 1:2897314/1‑71 (MQ=255)
attGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGAc > 1:768009/1‑46 (MQ=255)
attGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCt > 1:2396631/1‑51 (MQ=255)
attGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGTCTCAGGTAc > 1:1888870/1‑62 (MQ=255)
attGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTAc > 1:2407388/1‑62 (MQ=255)
attGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACt > 1:982808/1‑63 (MQ=255)
attGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTcttact > 1:1360360/1‑71 (MQ=255)
attGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTcttact > 1:3176244/1‑71 (MQ=255)
attGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTcttact > 1:735603/1‑71 (MQ=255)
attGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTcttact > 1:3447390/1‑71 (MQ=255)
attGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTcttact > 1:3496077/1‑71 (MQ=255)
attGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTcttact > 1:2436783/1‑71 (MQ=255)
attGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTcttact > 1:4630373/1‑71 (MQ=255)
attGTTGATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTctta > 1:66488/1‑69 (MQ=255)
ttgttgATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTcttact < 1:4214053/70‑1 (MQ=255)
gttgATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTcttactta < 1:240889/70‑1 (MQ=255)
gttgATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTcttactta < 1:4382920/70‑1 (MQ=255)
gttgATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCttacttac > 1:1469151/1‑71 (MQ=255)
ttgATGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACg < 1:58788/71‑1 (MQ=255)
tGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACttt < 1:2821744/57‑1 (MQ=255)
tGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATc < 1:1035433/71‑1 (MQ=255)
tGGAAGAACGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATc < 1:4452140/71‑1 (MQ=255)
gaagaaCGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATcc < 1:4550828/70‑1 (MQ=255)
agaaCGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTctt < 1:4428722/56‑1 (MQ=255)
aCGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTg < 1:1729147/70‑1 (MQ=255)
aCGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGa < 1:1038273/71‑1 (MQ=255)
aCGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGa < 1:3707499/71‑1 (MQ=255)
cGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGAc > 1:977045/1‑71 (MQ=255)
cGTGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGAc < 1:1587476/71‑1 (MQ=255)
tGCGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGt < 1:952896/71‑1 (MQ=255)
cGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGtt < 1:1875367/70‑1 (MQ=255)
cGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGtt < 1:2532533/70‑1 (MQ=255)
cGACCATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTa > 1:3063786/1‑71 (MQ=255)
ccATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTg < 1:2968778/71‑1 (MQ=255)
cATCGCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGtt > 1:3459628/1‑66 (MQ=255)
gCCCGCGTACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCg < 1:3689776/71‑1 (MQ=255)
gCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGc < 1:3345577/70‑1 (MQ=255)
gCCCGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCg > 1:1496661/1‑71 (MQ=255)
ccGCGAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGt < 1:2569319/70‑1 (MQ=255)
cgcgAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTc < 1:4322682/71‑1 (MQ=255)
gAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGAt < 1:1983970/71‑1 (MQ=255)
gAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGAt < 1:4193090/71‑1 (MQ=255)
gAACTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGAt < 1:1828424/71‑1 (MQ=255)
gAACTGCACGACTCGCTGGCTCAAGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGAt < 1:4085778/71‑1 (MQ=255)
|
TTAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGAT > W3110S.gb/2585423‑2585558
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
