Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,802,461 |
G→A |
A102T (GCC→ACC) |
nrdF → |
ribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,802,461 | 0 | G | A | 98.7%
| 219.9
/ ‑4.2
| 74 | A102T (GCC→ACC) | nrdF | ribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (30/43); total (31/43) |
| Fisher's exact test for biased strand distribution p-value = 4.19e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 |
CCGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTG > W3110S.gb/2802396‑2802528
|
ccGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGc < 1:2616106/71‑1 (MQ=255)
ccGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGc < 1:2304077/71‑1 (MQ=255)
ccGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGc < 1:3540897/71‑1 (MQ=255)
cACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTa > 1:1885642/1‑70 (MQ=255)
cACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGTAGCGGTTCATACCCGCTCTTACAGt > 1:2442085/1‑70 (MQ=255)
cACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGt > 1:1651071/1‑70 (MQ=255)
cATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGAttt > 1:3270638/1‑70 (MQ=255)
gaagaaGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTtctc > 1:3650990/1‑71 (MQ=255)
aagaagCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCg > 1:3975042/1‑62 (MQ=255)
aagaagCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTtctc > 1:3926340/1‑70 (MQ=255)
aagaagCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTtctc > 1:2642164/1‑70 (MQ=255)
aagaagCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTtctc > 1:3624655/1‑70 (MQ=255)
aagaagCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTtct > 1:4471758/1‑69 (MQ=255)
aagaagCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTtct > 1:3301730/1‑69 (MQ=255)
agCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGc < 1:460821/71‑1 (MQ=255)
agCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGc < 1:535315/71‑1 (MQ=255)
gCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCt < 1:4316478/71‑1 (MQ=255)
gCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCt < 1:2477677/71‑1 (MQ=255)
tattatCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGc < 1:2037606/71‑1 (MQ=255)
attatCAAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGc > 1:235004/1‑70 (MQ=255)
ttatCGAATATCAGCTTTATGGAAGCGGTTCATAcc > 1:4373577/1‑36 (MQ=255)
ttatCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTAc < 1:1868976/45‑1 (MQ=255)
ttatCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGc < 1:1840793/64‑1 (MQ=255)
ttatCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGc < 1:1302365/64‑1 (MQ=255)
ttatCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGc < 1:783020/64‑1 (MQ=255)
ttatCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGc < 1:4629129/64‑1 (MQ=255)
ttatCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGc < 1:1661491/64‑1 (MQ=255)
ttatCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGc > 1:3251722/1‑69 (MQ=255)
atCGAATATCAGCTTTATGGAAGCGGTTCATACCCGctc > 1:640407/1‑39 (MQ=255)
atCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGa > 1:3401730/1‑50 (MQ=255)
atCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGa > 1:3737678/1‑71 (MQ=255)
atCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGa > 1:3030599/1‑71 (MQ=255)
atCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGa > 1:2682377/1‑71 (MQ=255)
atCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGa > 1:1554441/1‑71 (MQ=255)
atCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGa > 1:2075280/1‑71 (MQ=255)
atCGAATATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGa > 1:2096439/1‑71 (MQ=255)
cGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTa > 1:240711/1‑40 (MQ=255)
aaTATCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGc < 1:1359116/58‑1 (MQ=255)
atatCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCaaa < 1:924798/71‑1 (MQ=255)
atatCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCaaa < 1:4559116/71‑1 (MQ=255)
atatCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCaaa < 1:1985747/71‑1 (MQ=255)
atatCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCaaa < 1:3475734/71‑1 (MQ=255)
atatCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCaaa < 1:3439165/71‑1 (MQ=255)
atatCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCaaa < 1:214844/71‑1 (MQ=255)
atatCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCaaa < 1:2943449/71‑1 (MQ=255)
tatCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCa > 1:1624593/1‑68 (MQ=255)
tCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTa > 1:107953/1‑56 (MQ=255)
tCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGAt < 1:247153/71‑1 (MQ=255)
tCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGAt > 1:2053559/1‑71 (MQ=255)
tCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGAt < 1:3101157/71‑1 (MQ=255)
tCAGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGAt > 1:3110854/1‑71 (MQ=255)
aGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGa > 1:3247521/1‑49 (MQ=255)
aGCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGc > 1:4558681/1‑52 (MQ=255)
gCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCaaa < 1:1713630/65‑1 (MQ=255)
gCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTc < 1:1448622/71‑1 (MQ=255)
gCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTc < 1:510176/71‑1 (MQ=255)
gCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTc < 1:2717497/71‑1 (MQ=255)
gCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTc < 1:3017186/71‑1 (MQ=255)
gCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTc < 1:2528591/71‑1 (MQ=255)
gCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTc < 1:4399195/71‑1 (MQ=255)
gCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTc < 1:171461/71‑1 (MQ=255)
gCTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTc < 1:3908174/71‑1 (MQ=255)
cTTTATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTc < 1:3570337/70‑1 (MQ=255)
cTTTATGGAAGCGGTTCATACACGCTCTTACAGTTCGATTTTCTCGACGc < 1:4298971/50‑1 (MQ=255)
tttATTGAAGCGGTTCATACCCGCTCTTACAGTTCTATTTTCTCGACGCTATGCCAGACCAAAGATGTc < 1:1797417/69‑1 (MQ=255)
tttATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGc < 1:424809/49‑1 (MQ=255)
tttATGGAAGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTc < 1:4196408/69‑1 (MQ=255)
aaGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGGCGATgccgcc < 1:3324781/71‑1 (MQ=255)
aGCGGTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTc < 1:1404171/61‑1 (MQ=255)
ggTTCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTCGATGCCGCCTACg > 1:2126107/1‑71 (MQ=255)
ttCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCaaa < 1:2891648/50‑1 (MQ=255)
ttCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTCGATGCCGCCTACGcc < 1:3035371/71‑1 (MQ=255)
ttCATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTCGATGCCGCCTACGcc < 1:3040780/71‑1 (MQ=255)
cATACCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTg > 1:1747527/1‑71 (MQ=255)
|
CCGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTG > W3110S.gb/2802396‑2802528
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
