Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I1 R2 328 63.0 4687567 98.0% 4593815 65.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,567,710 T→C R102R (CGT→CGC)  yihQ → alpha‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,567,7100TC100.0% 264.1 / NA 75R102R (CGT→CGC) yihQalpha‑glucosidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (54/21);  total (54/21)

TAATTCATTTCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGTTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGCCGCTCAACCAG  >  W3110S.gb/3567643‑3567780
                                                                   |                                                                      
tAATTCATTTCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTa                                                                     >  1:1397025/1‑71 (MQ=255)
tAATTCATTTCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTa                                                                     >  1:1934341/1‑71 (MQ=255)
tAATTCATTTCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTa                                                                     >  1:985118/1‑71 (MQ=255)
 aaTTCATTTCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTAt                                                                    >  1:4145968/1‑71 (MQ=255)
  aTTCATTTCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTAtt                                                                   <  1:3824418/71‑1 (MQ=255)
  aTTCATTTCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTAt                                                                    <  1:3471993/70‑1 (MQ=255)
     cATTTCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCt                                                                >  1:3542012/1‑71 (MQ=255)
     cATTTCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCt                                                                >  1:45497/1‑71 (MQ=255)
     cATTTCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCt                                                                >  1:2582942/1‑71 (MQ=255)
     cATTTCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCt                                                                >  1:3227921/1‑71 (MQ=255)
     cATTTCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCt                                                                >  1:4328029/1‑71 (MQ=255)
       tttCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCttg                                                              <  1:4338529/71‑3 (MQ=255)
         tCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGaa                                                            >  1:326034/1‑71 (MQ=255)
         tCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGa                                                             <  1:1356553/70‑1 (MQ=255)
                  gTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACaaaa                                                    >  1:3232411/1‑70 (MQ=255)
                  gTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAAc                                                   >  1:4301113/1‑71 (MQ=255)
                    tCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGa                                                 >  1:3161309/1‑71 (MQ=255)
                     cTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGa                                                 >  1:4305845/1‑70 (MQ=255)
                     cTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGa                                                 >  1:3955054/1‑70 (MQ=255)
                         cATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAAc                                             <  1:512348/70‑1 (MQ=255)
                           ttAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTgg                                                              <  1:2312521/51‑1 (MQ=255)
                           ttAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAATACGACAACCtt                                          <  1:2309832/71‑1 (MQ=255)
                             aGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACaa                                              >  1:241989/1‑65 (MQ=255)
                             aGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACaa                                              >  1:3702486/1‑65 (MQ=255)
                             aGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCtt                                          >  1:2642601/1‑69 (MQ=255)
                             aGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCt                                           >  1:1374640/1‑68 (MQ=255)
                             aGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTaa                                        >  1:1863357/1‑71 (MQ=255)
                             aGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTaa                                        >  1:2476652/1‑71 (MQ=255)
                             aGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTaa                                        >  1:3205460/1‑71 (MQ=255)
                             aGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTaa                                        >  1:3539956/1‑71 (MQ=255)
                             aGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTaa                                        >  1:2580662/1‑71 (MQ=255)
                             aGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTaa                                        >  1:284753/1‑71 (MQ=255)
                             aGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTaa                                        >  1:2772504/1‑71 (MQ=255)
                                      cTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCg                               >  1:4431736/1‑71 (MQ=255)
                                      cTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCg                               >  1:2711217/1‑71 (MQ=255)
                                      cTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCg                               <  1:1397788/71‑1 (MQ=255)
                                      cTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCg                               >  1:4359588/1‑71 (MQ=255)
                                      cTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCg                               >  1:1688586/1‑71 (MQ=255)
                                      cTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCg                               <  1:1811467/71‑1 (MQ=255)
                                      cTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAA‑CACAACCg                               >  1:2667485/1‑70 (MQ=255)
                                         aaTATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTa                             <  1:2481000/70‑1 (MQ=255)
                                          atatCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACcac                                    <  1:692505/62‑1 (MQ=255)
                                           tatCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCtt                                          <  1:3107182/55‑1 (MQ=255)
                                           tatCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCtt                                          <  1:1028285/55‑1 (MQ=255)
                                           tatCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAAcc                                >  1:2544461/1‑65 (MQ=255)
                                           tatCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCt                          >  1:1734455/1‑71 (MQ=255)
                                           tatCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCt                          >  1:1211828/1‑71 (MQ=255)
                                             tctcTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTac                                        >  1:1085991/1‑54 (MQ=255)
                                             tctcTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTAt                            >  1:4659539/1‑67 (MQ=255)
                                             tctcTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTgg                        >  1:3928976/1‑71 (MQ=255)
                                             tctcTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTgg                        >  1:2776745/1‑71 (MQ=255)
                                             tctcTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTgg                        >  1:4676352/1‑71 (MQ=255)
                                             tctcTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTg                         <  1:1202464/70‑1 (MQ=255)
                                             tctcTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACATTAACCACAACCGTATCTgg                        >  1:2675092/1‑71 (MQ=255)
                                              ctctGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGc                       >  1:2477289/1‑71 (MQ=255)
                                               tctGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGAc                                                >  1:3304406/1‑45 (MQ=255)
                                               tctGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCt                      >  1:138317/1‑71 (MQ=255)
                                               tctGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCt                      >  1:3177217/1‑71 (MQ=255)
                                                   ccGACGATCAGGTGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTgcgc                  <  1:2511382/71‑1 (MQ=255)
                                                   ccGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGa                                                 >  1:1596849/1‑40 (MQ=255)
                                                   ccGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTgcg                   <  1:372291/70‑1 (MQ=255)
                                                   ccGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTgcg                   <  1:3285967/70‑1 (MQ=255)
                                                     gacgaTCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCt                <  1:2723551/71‑1 (MQ=255)
                                                     gacgaTCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCt                <  1:4425350/71‑1 (MQ=255)
                                                         aTCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTa                             <  1:1914594/54‑1 (MQ=255)
                                                         aTCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTa                             <  1:1197476/54‑1 (MQ=255)
                                                           cAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTg                         >  1:908563/1‑56 (MQ=255)
                                                           cAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGTCTGCGCCTTGCCGc          >  1:3520912/1‑71 (MQ=255)
                                                           cAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGCCg           >  1:3994861/1‑70 (MQ=255)
                                                           cAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGCCGc          >  1:2444380/1‑71 (MQ=255)
                                                           cAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGCCGc          >  1:2535678/1‑71 (MQ=255)
                                                           cAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGCCGc          >  1:4208855/1‑71 (MQ=255)
                                                           cAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGCCGc          >  1:2914754/1‑71 (MQ=255)
                                                           cAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCCGGCTGCGCCTTGCCGc          >  1:3781014/1‑71 (MQ=255)
                                                               ggCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGCCGCTCaa      >  1:3898501/1‑71 (MQ=255)
                                                                   tttATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGCCGCTCAACCag  <  1:15160/71‑1 (MQ=255)
                                                                   tttATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGCCGCTCAACCag  <  1:2989212/71‑1 (MQ=255)
                                                                   |                                                                      
TAATTCATTTCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGTTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGCCGCTCAACCAG  >  W3110S.gb/3567643‑3567780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A