Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,712,785 |
T→C |
V579A (GTA→GCA) |
gidA → |
glucose‑inhibited cell‑division protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,712,785 | 0 | T | C | 100.0%
| 122.7
/ NA
| 37 | V579A (GTA→GCA) | gidA | glucose‑inhibited cell‑division protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (12/25); total (12/25) |
GATGAGATCGAAAAGCAGCTGCGTAACGAGAACACCCTGCTACCCGCGACACTGGATTACCGCCAGGTATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGCCTCTATCGGC > W3110S.gb/3712718‑3712846
|
gATGAGATCGAAAAGCAGCTGCGTAACGAGAACACCCTGCTACCCGCGACACTGGATTACCGCCAGGCATc > 1:3098869/1‑71 (MQ=255)
tGAGATCGAAAAGCAGCTGCGTAACGAGAACACCCTGCTACCCGCGACACTGGATTACCGCCAGGCATCCg > 1:2372664/1‑71 (MQ=255)
agaTCGAAAAGCAGCTGCGTAACGAGAACACCCTGCTACCCGCGACACTGGATTACCGCCAGGCATCCGGt < 1:754784/71‑1 (MQ=255)
agaTCGAAAAGCAGCTGCGTAACGAGAACACCCTGCTACCCGCGACACTGGATTACCGCCAGGCATCCGGt < 1:546382/71‑1 (MQ=255)
tCGAAAAGCAGCTGCGTAACGAGAACACCCTGCTACCCGCGACACTGGATTACCGCCAGTCATCCGGTCtt < 1:3567485/71‑1 (MQ=255)
cagcTGCGTAACGAGAACACCCTGCTACCCGCGACACTGGATTACCGCCAGGCATCCGGTCTTTCTAACGa < 1:657203/71‑1 (MQ=255)
agcTGCGTAACGAGAACACCCTGCTACCCGCGACACTGGATTACCGCCAGGCATCCGGTCTTTCTAACGaa < 1:3478839/71‑1 (MQ=255)
gCGTAACGAGAACACCCTGCTACCCGCGACACTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGa > 1:4529232/1‑71 (MQ=255)
cGTAACGAGAACACCCTGCTACCCGCGACACTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTg < 1:3823796/69‑1 (MQ=255)
agaACACCCTGCTACCCGCGACACTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTg < 1:2892713/62‑1 (MQ=255)
acacCCTGCTACCCGCGACACTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACtt < 1:4229919/71‑1 (MQ=255)
acacCCTGCTACCCGCGACACTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACtt < 1:997135/71‑1 (MQ=255)
cacCCTGCTACCCGCGACACTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACtt > 1:4030953/1‑70 (MQ=255)
cccTGCTACCCGCGACACTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAAc > 1:937976/1‑71 (MQ=255)
ccTGCTACCCGGGAGTCTGTATTACCGCAAGGCATCCGGTCTTTCTAActa < 1:4623472/51‑3 (MQ=255)
atGCTACCCGCGACACTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGa > 1:950320/2‑71 (MQ=255)
gCTACCCGCGACACTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTg < 1:3129500/52‑1 (MQ=255)
cccGCGACACTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATcaca < 1:2026226/70‑1 (MQ=255)
cgACACTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCa < 1:3075191/71‑1 (MQ=255)
gACACTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAg < 1:4373640/71‑1 (MQ=255)
acacTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGc < 1:4644163/71‑1 (MQ=255)
acacTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGc < 1:1792166/71‑1 (MQ=255)
cacTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGc < 1:546895/70‑1 (MQ=255)
acTGGATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATcaca < 1:3328354/62‑1 (MQ=255)
ggATTACCGCCAGGCATCCGGTCTTTCTAACGAAg > 1:2038115/1‑35 (MQ=255)
ggATTACCGCCAGGCATCCGGTCTTTCTAACGAAg > 1:1965369/1‑35 (MQ=255)
gATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGCctct < 1:1436718/69‑1 (MQ=255)
gATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGCctct < 1:3195391/69‑1 (MQ=255)
gATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGCctct < 1:3131863/69‑1 (MQ=255)
gATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGCctct < 1:200303/69‑1 (MQ=255)
gATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGCctct < 1:1685023/69‑1 (MQ=255)
gATTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGCCTCTa < 1:4245353/70‑1 (MQ=255)
aTTACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGCctct < 1:2014446/68‑1 (MQ=255)
tACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGcc > 1:212888/1‑63 (MQ=255)
tACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGCCTCTATCgg > 1:893237/1‑71 (MQ=255)
tACCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGCCTATATCgg > 1:1971792/1‑71 (MQ=255)
aCCGCCAGGCATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGCCTCTATCGGc > 1:2682339/1‑71 (MQ=255)
|
GATGAGATCGAAAAGCAGCTGCGTAACGAGAACACCCTGCTACCCGCGACACTGGATTACCGCCAGGTATCCGGTCTTTCTAACGAAGTGATCGCCAAACTTAACGATCACAAACCAGCCTCTATCGGC > W3110S.gb/3712718‑3712846
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
