Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F3 I0 R2
|
232 |
76.3 |
4135488 |
90.9% |
3759158 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,850,177 |
G→A |
36.8% |
Q286* (CAG→TAG) |
yjeP ← |
predicted mechanosensitive channel |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,850,177 | 0 | G | A | 36.8%
| 51.3
/ 58.7
| 57 | Q286* (CAG→TAG) | yjeP | predicted mechanosensitive channel |
| Reads supporting (aligned to +/- strand): ref base G (21/15); new base A (11/10); total (32/25) |
| Fisher's exact test for biased strand distribution p-value = 7.84e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01 |
TGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATT > minE/2850110‑2850244
|
tGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTg > 1:3621087/1‑71 (MQ=255)
gCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGc > 1:1075304/1‑71 (MQ=255)
gCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGc > 1:490937/1‑71 (MQ=255)
gCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGc > 1:1835419/1‑71 (MQ=255)
gCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGc > 1:259373/1‑71 (MQ=255)
gCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGc > 1:2488568/1‑71 (MQ=255)
gTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGc > 1:560946/1‑70 (MQ=255)
gTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGc > 1:190428/1‑70 (MQ=255)
gTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGc > 1:3868450/1‑70 (MQ=255)
ttCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGc < 1:1940961/69‑1 (MQ=255)
ttCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCaa > 1:564117/1‑71 (MQ=255)
ttCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGc < 1:1335028/69‑1 (MQ=255)
ttCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGc < 1:549357/69‑1 (MQ=255)
ttCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGc < 1:3584436/69‑1 (MQ=255)
cACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAAc > 1:1581767/1‑70 (MQ=255)
aCGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACg < 1:993786/70‑1 (MQ=255)
aCGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACga < 1:788153/71‑1 (MQ=255)
aCGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACga < 1:2577874/71‑1 (MQ=255)
aCGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACga < 1:3815338/71‑1 (MQ=255)
aCGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACga < 1:1691244/71‑1 (MQ=255)
cAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGat < 1:436966/71‑1 (MQ=255)
aGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGat < 1:150940/70‑1 (MQ=255)
gCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatcc < 1:744112/71‑1 (MQ=255)
tATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatc < 1:3831861/67‑1 (MQ=255)
ttCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCg > 1:525007/1‑71 (MQ=255)
cAACGCGTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCt > 1:530837/1‑71 (MQ=255)
cAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGc > 1:2775253/1‑70 (MQ=255)
cAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCt > 1:1812018/1‑71 (MQ=255)
cAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCt > 1:3595580/1‑71 (MQ=255)
cAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCt > 1:2981497/1‑71 (MQ=255)
cAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCt > 1:2725512/1‑71 (MQ=255)
cAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCt > 1:3671733/1‑71 (MQ=255)
aCGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTgc < 1:625222/71‑1 (MQ=255)
aCGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTgc < 1:2611159/71‑1 (MQ=255)
aCGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTgc < 1:2165789/71‑1 (MQ=255)
ggACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGa < 1:947085/71‑1 (MQ=255)
aCCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatc < 1:3205760/50‑1 (MQ=255)
gTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCa > 1:1715366/1‑69 (MQ=255)
gTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCa > 1:1983068/1‑69 (MQ=255)
aCGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgcc > 1:1001070/1‑71 (MQ=255)
aCGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgcc > 1:3857520/1‑71 (MQ=255)
aCGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgcc > 1:2411858/1‑71 (MQ=255)
gTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGAt > 1:2311450/1‑61 (MQ=255)
tCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgccgcc < 1:394436/71‑1 (MQ=255)
tCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgccgcc < 1:2399883/71‑1 (MQ=255)
tCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgccgcc < 1:8452/71‑1 (MQ=255)
tCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgccgcc < 1:814750/71‑1 (MQ=255)
tCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgccgcc < 1:3750766/71‑1 (MQ=255)
tggctggcAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgccgc > 1:201801/1‑68 (MQ=255)
gctggcAGCCTGACGCTGTTGCGAGGCAAGGAGATCCATCCGCTGCGCCTGTTGATTCAAAGccgccg < 1:417307/68‑1 (MQ=255)
gctggcAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGccgccg < 1:3913805/68‑1 (MQ=255)
ctggcAGCCTGACGCTGTTGCGAGGCAACGAGatccat > 1:2153457/1‑38 (MQ=255)
gcAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGa > 1:2019684/1‑65 (MQ=255)
gcAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGa > 1:3386023/1‑65 (MQ=255)
gcAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGa > 1:2638596/1‑65 (MQ=255)
gCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAAtt > 1:3648183/1‑71 (MQ=255)
gCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAAtt > 1:3799361/1‑71 (MQ=255)
|
TGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATT > minE/2850110‑2850244
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
