Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F8 I0 R2
|
296 |
77.6 |
4260404 |
88.1% |
3753415 |
61.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,850,177 |
G→A |
54.2% |
Q286* (CAG→TAG) |
yjeP ← |
predicted mechanosensitive channel |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,850,177 | 0 | G | A | 54.2%
| 8.2
/ 83.3
| 59 | Q286* (CAG→TAG) | yjeP | predicted mechanosensitive channel |
| Reads supporting (aligned to +/- strand): ref base G (12/15); new base A (10/22); total (22/37) |
| Fisher's exact test for biased strand distribution p-value = 4.18e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01 |
GCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTT > minE/2850111‑2850233
|
gCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTg > 1:3618345/1‑70 (MQ=255)
gCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGc > 1:1730780/1‑71 (MQ=255)
gCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGc > 1:478974/1‑71 (MQ=255)
gCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGc > 1:3338752/1‑71 (MQ=255)
cGACTGTTCACGCCGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGc < 1:4180325/70‑1 (MQ=255)
cGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGc < 1:2646403/70‑1 (MQ=255)
cGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGc < 1:3365216/70‑1 (MQ=255)
cGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGc < 1:2030038/70‑1 (MQ=255)
cGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGc < 1:2093533/70‑1 (MQ=255)
cGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGc < 1:1003372/70‑1 (MQ=255)
cGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGc < 1:3865448/70‑1 (MQ=255)
aCTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAg > 1:3357277/1‑71 (MQ=255)
aCTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAg > 1:2604285/1‑71 (MQ=255)
aCTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAg > 1:807660/1‑71 (MQ=255)
cACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGc > 1:2193155/1‑67 (MQ=255)
cACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAAc > 1:642924/1‑70 (MQ=255)
cACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAAc > 1:2340251/1‑70 (MQ=255)
aCGCAGCGTATTCAACGCCTGGCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACga < 1:809738/71‑1 (MQ=255)
aCGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACg < 1:2419821/70‑1 (MQ=255)
aGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTTACGCTGTTTCGAGGCAACGAGatc < 1:2069313/71‑1 (MQ=255)
aGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatc < 1:2041719/71‑1 (MQ=255)
aGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatc < 1:1997396/71‑1 (MQ=255)
cGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatcca > 1:2403419/1‑71 (MQ=255)
cGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGatcca < 1:3899079/71‑1 (MQ=255)
cGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGatcca < 1:1025603/71‑1 (MQ=255)
ttCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatccatc > 1:1885775/1‑69 (MQ=255)
ttCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatccatc > 1:3791983/1‑69 (MQ=255)
ttCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatcca < 1:982659/67‑1 (MQ=255)
ttCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatcca < 1:309432/67‑1 (MQ=255)
ttCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatcca < 1:4107742/67‑1 (MQ=255)
ttCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatcca < 1:4140600/67‑1 (MQ=255)
ttCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatcca < 1:255105/67‑1 (MQ=255)
ttCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCg > 1:2166332/1‑71 (MQ=255)
cTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCt < 1:4020319/71‑1 (MQ=255)
cTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCt < 1:1640104/71‑1 (MQ=255)
cTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCt < 1:2309024/71‑1 (MQ=255)
gCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTATTGc < 1:289316/41‑1 (MQ=255)
cTGTAACGTCTGGCTTGCAGCCTGACGCTATTGCGAGGCAACGAGatcc < 1:3923796/49‑1 (MQ=255)
cTGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGatcc < 1:2024357/49‑1 (MQ=255)
tGTAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGatcc < 1:568725/48‑1 (MQ=255)
tAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTgcgc < 1:3098108/57‑1 (MQ=255)
tAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCaaa < 1:381688/70‑1 (MQ=255)
tAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCaaa < 1:1836535/70‑1 (MQ=255)
tAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAg > 1:930882/1‑71 (MQ=255)
tAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGat > 1:217206/1‑44 (MQ=255)
tAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTc < 1:62665/67‑1 (MQ=255)
tAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTc < 1:3557462/67‑1 (MQ=255)
tAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTc < 1:282300/67‑1 (MQ=255)
tAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAg > 1:406261/1‑71 (MQ=255)
tAACGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCAGCTGCGCCTGTTGATTCAAAg > 1:1943040/1‑71 (MQ=255)
aaCGTCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgc > 1:2305558/1‑71 (MQ=255)
aCGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgcc > 1:1302830/1‑71 (MQ=255)
tCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgccgcc < 1:551267/71‑1 (MQ=255)
tCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgccgcc < 1:3047657/71‑1 (MQ=255)
tCTGGCTGGCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgccgcc < 1:3731123/71‑1 (MQ=255)
ctggctggCAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgccgcc < 1:2540810/70‑1 (MQ=255)
tggctggcAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTc > 1:2327813/1‑60 (MQ=255)
gctggcAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGa > 1:1572663/1‑69 (MQ=255)
gcAGCCTGACGCTATTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGtt < 1:677940/70‑1 (MQ=255)
|
GCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTT > minE/2850111‑2850233
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
