Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I1 R1 122 92.7 4382561 94.3% 4132755 66.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 1,664,529 G→A I231I (ATC→ATT)  guaA ← GMP synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,664,5290GA100.0% 370.8 / NA 110I231I (ATC→ATT) guaAGMP synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (63/47);  total (63/47)

Only 100 of 110 total aligned reads displayed.

TTTTTACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGGATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCATCGTCGATAATTTTCGCTGG  >  minE/1664461‑1664594
                                                                    |                                                                 
tttttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAAt                                                                 >  1:595257/1‑71 (MQ=255)
tttttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAAt                                                                 >  1:3414152/1‑71 (MQ=255)
tttttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAAt                                                                 >  1:4059049/1‑71 (MQ=255)
  tttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACTGAGGAATCCACACCACCAGAGAGGCCGAGAATGa                                                               >  1:528979/1‑71 (MQ=255)
  tttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAAt                                                                 >  1:3910652/1‑69 (MQ=255)
  tttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGa                                                               >  1:2291970/1‑71 (MQ=255)
  tttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGa                                                               >  1:4269083/1‑71 (MQ=255)
           aGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTAtcgt                                                      >  1:1428195/1‑71 (MQ=255)
           aGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTAtcgt                                                      >  1:3180534/1‑71 (MQ=255)
           aGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTAtcgt                                                      >  1:3938472/1‑71 (MQ=255)
           aGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTAtcgt                                                      >  1:1164793/1‑71 (MQ=255)
           aGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTAtcgt                                                      >  1:772388/1‑71 (MQ=255)
           aGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTAtcg                                                       >  1:1243553/1‑70 (MQ=255)
                        agcaTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCTAGAATGACTTTATCGTCGCCTACCTGCTc                                         >  1:1916093/1‑71 (MQ=255)
                        agcaTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGc                                           >  1:3079521/1‑69 (MQ=255)
                        agcaTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCt                                          >  1:4329077/1‑70 (MQ=255)
                        agcaTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTc                                         >  1:3261202/1‑71 (MQ=255)
                        agcaTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTc                                         >  1:853671/1‑71 (MQ=255)
                        agcaTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTc                                         >  1:255575/1‑71 (MQ=255)
                        agcaTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTc                                         >  1:2609195/1‑71 (MQ=255)
                        agcaTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTc                                         >  1:2851984/1‑71 (MQ=255)
                        agcaTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTc                                         >  1:3021851/1‑71 (MQ=255)
                        agcaTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTc                                         >  1:4063565/1‑71 (MQ=255)
                        agcaTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTc                                         >  1:3629371/1‑71 (MQ=255)
                        agcaTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTc                                         >  1:3814706/1‑71 (MQ=255)
                        agcaTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTc                                         >  1:3106512/1‑71 (MQ=255)
                        agcaTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTc                                         >  1:4158680/1‑71 (MQ=255)
                        agcaTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTc                                         >  1:1038600/1‑71 (MQ=255)
                        agcaTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTc                                         >  1:1215844/1‑71 (MQ=255)
                        agcaTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTc                                         >  1:1254765/1‑71 (MQ=255)
                        agcaTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTc                                         >  1:135194/1‑71 (MQ=255)
                        agcaTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTc                                         >  1:1711450/1‑71 (MQ=255)
                        agcaTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATCACTTTATCGTCGCCTACCTGCTc                                         >  1:611742/1‑71 (MQ=255)
                         gcaTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTcg                                        <  1:1668547/71‑1 (MQ=255)
                         gcaTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTcg                                        <  1:328697/71‑1 (MQ=255)
                         gcaTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTcg                                        <  1:259262/71‑1 (MQ=255)
                         gcaTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTcg                                        <  1:2832292/71‑1 (MQ=255)
                           aTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTcgcg                                      <  1:2121728/71‑1 (MQ=255)
                           aTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTcgcg                                      <  1:1050692/71‑1 (MQ=255)
                           aTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTcgcg                                      <  1:1064891/71‑1 (MQ=255)
                           aTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTcgcg                                      <  1:448094/71‑1 (MQ=255)
                           aTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTcgcg                                      <  1:2957712/71‑1 (MQ=255)
                           aTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTcgcg                                      <  1:2385554/71‑1 (MQ=255)
                            ttGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTcgcg                                      <  1:3579549/70‑1 (MQ=255)
                                 gTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTa                                                <  1:796890/55‑1 (MQ=255)
                                 gTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTa                                                <  1:1452953/55‑1 (MQ=255)
                                 gTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTa                                                <  1:1982387/55‑1 (MQ=255)
                                 gTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTa                                                <  1:3920244/55‑1 (MQ=255)
                                 gTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTa                                                <  1:4064956/55‑1 (MQ=255)
                                 gTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTa                                                <  1:1265014/55‑1 (MQ=255)
                                 gTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTa                                                <  1:1262497/55‑1 (MQ=255)
                                 gTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTa                                                <  1:2341286/55‑1 (MQ=255)
                                 gTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTa                                                <  1:2287283/55‑1 (MQ=255)
                                 gTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTa                                                <  1:61033/55‑1 (MQ=255)
                                 gTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCg                                <  1:4302206/71‑1 (MQ=255)
                                 gTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCg                                <  1:4252481/71‑1 (MQ=255)
                                 gTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCg                                <  1:3961944/71‑1 (MQ=255)
                                 gTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCg                                <  1:1424521/71‑1 (MQ=255)
                                 gTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCg                                <  1:2225152/71‑1 (MQ=255)
                                 gTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCg                                <  1:678027/71‑1 (MQ=255)
                                 gTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCg                                <  1:3765567/71‑1 (MQ=255)
                                 gTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCg                                <  1:193147/71‑1 (MQ=255)
                                 gTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCg                                <  1:3078679/71‑1 (MQ=255)
                                 gTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCg                                <  1:1035287/71‑1 (MQ=255)
                                 gTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCg                                <  1:3173093/71‑1 (MQ=255)
                                 gTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCg                                <  1:3519785/71‑1 (MQ=255)
                                 gTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCg                                <  1:1715212/71‑1 (MQ=255)
                                 gTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCg                                <  1:2982208/71‑1 (MQ=255)
                                 gTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCg                                <  1:1951394/71‑1 (MQ=255)
                                  ttACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTa                                                <  1:2117325/54‑1 (MQ=255)
                                  ttACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTa                                                <  1:311294/54‑1 (MQ=255)
                                          gaATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAg                        >  1:1876824/1‑70 (MQ=255)
                                          gaATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAg                        >  1:2162056/1‑70 (MQ=255)
                                          gaATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAg                        >  1:3218701/1‑70 (MQ=255)
                                          gaATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAg                        >  1:158297/1‑70 (MQ=255)
                                          gaATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAg                        >  1:1708548/1‑70 (MQ=255)
                                          gaATCCACACCACCAGACAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAg                        >  1:3630842/1‑70 (MQ=255)
                                          gaATACACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAg                        >  1:2791885/1‑70 (MQ=255)
                                                 caccacCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgtc                 >  1:3187031/1‑70 (MQ=255)
                                                 caccacCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgtc                 >  1:3679239/1‑70 (MQ=255)
                                                 caccacCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgtc                 >  1:3867667/1‑70 (MQ=255)
                                                 caccacCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgtc                 >  1:707093/1‑70 (MQ=255)
                                                 caccacCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgtc                 >  1:3934027/1‑70 (MQ=255)
                                                  accaccAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGa                               >  1:521213/1‑55 (MQ=255)
                                                  accaccAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAg                              >  1:1887511/1‑56 (MQ=255)
                                                  accaccAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAg                              >  1:2086397/1‑56 (MQ=255)
                                                  accaccAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAg                              >  1:847526/1‑56 (MQ=255)
                                                  accaccAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAg                              >  1:4201180/1‑56 (MQ=255)
                                                  accaccAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAg                              >  1:4326154/1‑56 (MQ=255)
                                                  accaccAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAg                              >  1:2444229/1‑56 (MQ=255)
                                                  accaccAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAg                              >  1:3996614/1‑56 (MQ=255)
                                                  accaccAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAg                              >  1:3880350/1‑56 (MQ=255)
                                                  accaccAGACAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAg                              >  1:2522825/1‑56 (MQ=255)
                                                       caGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCg                                <  1:3242471/49‑1 (MQ=255)
                                                             ggCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCATCGTCGATAATTTTCGCt    <  1:3575735/71‑1 (MQ=255)
                                                             ggCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCATCGTCGATAATTTTCGCt    <  1:684496/71‑1 (MQ=255)
                                                             ggCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCATCGTCGATAATTTTCGCt    <  1:3610404/71‑1 (MQ=255)
                                                              gCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCATCGTCGATAAttt         >  1:306689/1‑65 (MQ=255)
                                                              gCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCATCGTCGA‑AATTTTCGCTgg  >  1:1350556/1‑71 (MQ=255)
                                                               ccGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCATCGTCGATAATTTTCGCt    <  1:2749281/69‑1 (MQ=255)
                                                                    |                                                                 
TTTTTACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGGATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCATCGTCGATAATTTTCGCTGG  >  minE/1664461‑1664594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A