Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F21 I0 R1
|
57 |
0.0 |
661283 |
90.4% |
597799 |
68.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
584,195 |
Δ2 bp |
100% |
coding (1699‑1700/1722 nt) |
cydC ← |
fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 584,195 | 0 | T | . | 100.0%
| 105.8
/ NA
| 24 | coding (1700/1722 nt) | cydC | fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (24/0); total (24/0) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
| * | minE | 584,196 | 0 | A | . | 100.0%
| 105.8
/ NA
| 24 | coding (1699/1722 nt) | cydC | fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (24/0); total (24/0) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCT > minE/584135‑584207
||
cGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGG‑‑ATAACGcccc > 1:449167/1‑70 (MQ=255)
cGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGG‑‑ATAACGcccc > 1:277501/1‑70 (MQ=255)
cGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGG‑‑ATAACGCCCCt > 1:3251/1‑71 (MQ=255)
cGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGG‑‑ATAACGCCCCt > 1:56052/1‑71 (MQ=255)
cGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGG‑‑ATAACGCCCCt > 1:517465/1‑71 (MQ=255)
cGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGG‑‑ATAACGCCCCt > 1:491557/1‑71 (MQ=255)
cGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGG‑‑ATAACGCCCCt > 1:473084/1‑71 (MQ=255)
cGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGG‑‑ATAACGCCCCt > 1:467984/1‑71 (MQ=255)
cGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGG‑‑ATAACGCCCCt > 1:436653/1‑71 (MQ=255)
cGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGG‑‑ATAACGCCCCt > 1:419314/1‑71 (MQ=255)
cGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGG‑‑ATAACGCCCCt > 1:400750/1‑71 (MQ=255)
cGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGG‑‑ATAACGCCCCt > 1:369699/1‑71 (MQ=255)
cGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGG‑‑ATAACGCCCCt > 1:334731/1‑71 (MQ=255)
cGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGG‑‑ATAACGCCCCt > 1:126098/1‑71 (MQ=255)
cGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGG‑‑ATAACGCCCCt > 1:298701/1‑71 (MQ=255)
cGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGG‑‑ATAACGCCCCt > 1:277373/1‑71 (MQ=255)
cGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGG‑‑ATAACGCCCCt > 1:26508/1‑71 (MQ=255)
cGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGG‑‑ATAACGCCCCt > 1:264381/1‑71 (MQ=255)
cGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGG‑‑ATAACGCCCCt > 1:252133/1‑71 (MQ=255)
cGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGG‑‑ATAACGCCCCt > 1:22832/1‑71 (MQ=255)
cGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGG‑‑ATAACGCCCCt > 1:227053/1‑71 (MQ=255)
cGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGG‑‑ATAACGCCCCt > 1:218886/1‑71 (MQ=255)
cGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGG‑‑ATAACGCCCCt > 1:184947/1‑71 (MQ=255)
cGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGG‑‑ATAACGCCCCt > 1:154643/1‑71 (MQ=255)
||
CGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCT > minE/584135‑584207
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
