Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
584,788 |
T→A |
66.7% |
I369I (ATA→ATT) |
cydC ← |
fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 584,788 | 0 | T | A | 66.7%
| 51.2
/ 36.8
| 54 | I369I (ATA→ATT) | cydC | fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base T (15/3); new base A (28/8); total (43/11) |
| Fisher's exact test for biased strand distribution p-value = 7.33e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01 |
GGTCCCATGCGCGGGTCAGCTGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGAAATCCCTTTAAGTGCCTGTTGAGATTGCTC > minE/584721‑584847
|
ggTCCCATGCGCGGGTCAGCTGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCta < 1:109590/69‑1 (MQ=255)
ggTCCCATGCGCGGGTCAGCTGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCta < 1:1874045/69‑1 (MQ=255)
ggTCCCATGCGCGGGTCAGCTGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCta < 1:2369885/69‑1 (MQ=255)
ggTCCCATGCGCGGGTCAGCTGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCta < 1:2389412/69‑1 (MQ=255)
ggTCCCATGCGCGGGTCAGCTGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCta < 1:1545235/69‑1 (MQ=255)
ggTCCCATGCGCGGGTCAGCTGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCta < 1:2659394/69‑1 (MQ=255)
ggTCCCATGCGCGGGTCAGCTGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCta < 1:2750141/69‑1 (MQ=255)
ggTCCCATGCGCGGGTCAGCTGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCta < 1:1251072/69‑1 (MQ=255)
ggTCCCATGCGCGGGTCAGCTGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCta < 1:341830/69‑1 (MQ=255)
gTCCCATGCGCGGGTCAGCTGTTGTAACAGTGTTGATTTGCCGCATCCGGTTAGCCCGAGAATCGCta < 1:3394160/68‑1 (MQ=255)
gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGCATCGCTATATGTTcccc > 1:1633486/1‑69 (MQ=255)
gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCAATATGTTcccc > 1:3415589/1‑69 (MQ=255)
gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCAATATGTTcccc > 1:2737789/1‑69 (MQ=255)
gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCAATATGTTcccc > 1:530842/1‑69 (MQ=255)
gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCAATATGTTcccc > 1:243907/1‑69 (MQ=255)
gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCAATATGTTcccc > 1:2423796/1‑69 (MQ=255)
gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCAATATGTTcccc > 1:650173/1‑69 (MQ=255)
gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCAATATGTTcccc > 1:88530/1‑69 (MQ=255)
gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCAATATGTTcccc > 1:1972102/1‑69 (MQ=255)
gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCAATATGTTcccc > 1:2380198/1‑69 (MQ=255)
gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCAATATGTTcccc > 1:1781266/1‑69 (MQ=255)
gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCAATATGTTcccc > 1:1766109/1‑69 (MQ=255)
gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCAATATGTTcccc > 1:1615290/1‑69 (MQ=255)
gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCAATATGTTcccc > 1:1632693/1‑69 (MQ=255)
gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCAATATGTTcccc > 1:1636598/1‑69 (MQ=255)
gcgGGTCAGCAGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCAATATGTTccc > 1:51493/1‑68 (MQ=255)
aGCTGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCta < 1:616924/52‑1 (MQ=255)
aGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGaa < 1:2023650/69‑1 (MQ=255)
aGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGaa < 1:1100124/69‑1 (MQ=255)
aGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGaa < 1:1286643/69‑1 (MQ=255)
gATTTGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGAAATccc > 1:949302/1‑68 (MQ=255)
tGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAagag > 1:966876/1‑57 (MQ=255)
tGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAgaga > 1:962986/1‑58 (MQ=255)
tGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAgaga > 1:1941431/1‑58 (MQ=255)
tGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAgaga > 1:1164450/1‑58 (MQ=255)
tGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAgaga > 1:1235738/1‑58 (MQ=255)
tGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAgaga > 1:2131580/1‑58 (MQ=255)
tGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAgaga > 1:3161298/1‑58 (MQ=255)
tGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAgaga > 1:1489610/1‑58 (MQ=255)
tGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAgaga > 1:2865872/1‑58 (MQ=255)
cGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGAAATCCCTTTAAGTGCCTg > 1:1682363/1‑67 (MQ=255)
cGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGAAATCCCTTTAAGTGCCTg > 1:866304/1‑67 (MQ=255)
cGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGAAATCCCTTTAAGTGCCTg > 1:2075250/1‑67 (MQ=255)
cGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGAAATCCCTTTAAGTGCCTg > 1:2914413/1‑67 (MQ=255)
ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:2093628/1‑57 (MQ=255)
ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:1845131/1‑57 (MQ=255)
ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:1046937/1‑57 (MQ=255)
ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:2151806/1‑57 (MQ=255)
ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:688586/1‑57 (MQ=255)
ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:113975/1‑57 (MQ=255)
ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:61813/1‑57 (MQ=255)
ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:2515739/1‑57 (MQ=255)
ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:1226913/1‑57 (MQ=255)
ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:2588093/1‑57 (MQ=255)
ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:2932022/1‑57 (MQ=255)
ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:2743267/1‑57 (MQ=255)
ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:2899566/1‑57 (MQ=255)
gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:482215/69‑1 (MQ=255)
gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:1299601/69‑1 (MQ=255)
gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:2464840/69‑1 (MQ=255)
gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:1539882/69‑1 (MQ=255)
gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:324056/69‑1 (MQ=255)
gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:2716144/69‑1 (MQ=255)
gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:2036439/69‑1 (MQ=255)
gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:220931/69‑1 (MQ=255)
|
GGTCCCATGCGCGGGTCAGCTGTTGTAACAGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGAAATCCCTTTAAGTGCCTGTTGAGATTGCTC > minE/584721‑584847
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
