Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F21 I0 R1
|
57 |
0.0 |
661283 |
90.4% |
597799 |
68.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,187,308 |
T→C |
100% |
P183P (CCA→CCG) |
nanT ← |
sialic acid transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,187,308 | 0 | T | C | 100.0%
| 98.2
/ NA
| 27 | P183P (CCA→CCG) | nanT | sialic acid transporter |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (0/27); total (0/27) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAG > minE/2187257‑2187326
|
ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCTATAAAg < 1:85/70‑1 (MQ=255)
ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg < 1:384491/70‑1 (MQ=255)
ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg < 1:643845/70‑1 (MQ=255)
ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg < 1:616813/70‑1 (MQ=255)
ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg < 1:658461/70‑1 (MQ=255)
ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg < 1:577643/70‑1 (MQ=255)
ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg < 1:500044/70‑1 (MQ=255)
ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg < 1:440352/70‑1 (MQ=255)
ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg < 1:439803/70‑1 (MQ=255)
ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg < 1:78997/70‑1 (MQ=255)
ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg < 1:433782/70‑1 (MQ=255)
ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg < 1:408150/70‑1 (MQ=255)
ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg < 1:361879/70‑1 (MQ=255)
ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg < 1:328195/70‑1 (MQ=255)
ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg < 1:30513/70‑1 (MQ=255)
ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg < 1:294342/70‑1 (MQ=255)
ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg < 1:251486/70‑1 (MQ=255)
ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg < 1:246203/70‑1 (MQ=255)
ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg < 1:88459/70‑1 (MQ=255)
ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg < 1:127351/70‑1 (MQ=255)
ccAGTCTTCCGCTTCCGGGATGTTTGTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg < 1:209661/70‑1 (MQ=255)
ccAGTCTTCCCCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAACTGCCGATAAAg < 1:197278/70‑1 (MQ=255)
cAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg < 1:153598/69‑1 (MQ=255)
cAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg < 1:583412/69‑1 (MQ=255)
cAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg < 1:225887/69‑1 (MQ=255)
tccgGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg < 1:417815/57‑1 (MQ=255)
ttttACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg < 1:43414/47‑1 (MQ=255)
|
CCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAG > minE/2187257‑2187326
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
