Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F21 I0 R1
|
57 |
0.0 |
661283 |
90.4% |
597799 |
68.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,632,970 |
A→G |
100% |
K421R (AAA→AGA) |
livM → |
leucine/isoleucine/valine transporter subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,632,970 | 0 | A | G | 100.0%
| 187.1
/ NA
| 49 | K421R (AAA→AGA) | livM | leucine/isoleucine/valine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (0/49); total (0/49) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAAAGGAGAGCAGGCAT > minE/2632914‑2632984
|
gCAGGGCTTGCTGCCCTTGACGCGCCCGCAACTGAAGCTGAAAATCGTCGCAGCGAGAGGAGAGCAGGCAt < 1:417971/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:472022/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:358837/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:379681/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:396606/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:406596/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:41890/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:441356/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:458831/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:464534/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:469061/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:109513/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:493864/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:505253/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:524105/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:575845/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:599090/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:612128/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:614359/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:73398/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:98026/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:318431/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:113645/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:133830/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:141287/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:147116/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:14759/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:184581/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:198764/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:213270/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:23318/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:253768/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:256524/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:257765/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:282020/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:284482/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:291341/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGAGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:349120/71‑1 (MQ=255)
gCAGGGCTTGATGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:525547/71‑1 (MQ=255)
gCAGGGCGTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:485022/71‑1 (MQ=255)
cAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:257518/70‑1 (MQ=255)
cAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:53769/70‑1 (MQ=255)
cAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:554039/70‑1 (MQ=255)
cAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:382689/70‑1 (MQ=255)
aGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:255082/69‑1 (MQ=255)
cccATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:72317/58‑1 (MQ=255)
aCGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:10250/52‑1 (MQ=255)
aCTGAAGCTGATAATCGGCGCAGCGAGAGGAGAGCAGGctt < 1:253387/41‑3 (MQ=25)
aGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:512375/36‑1 (MQ=255)
|
GCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAAAGGAGAGCAGGCAT > minE/2632914‑2632984
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
