Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F28 I3 R2
|
122 |
64.1 |
3058059 |
97.6% |
2984665 |
63.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
2,632,970 |
A→G |
K421R (AAA→AGA) |
livM → |
leucine/isoleucine/valine transporter subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,632,970 | 0 | A | G | 100.0%
| 157.0
/ NA
| 44 | K421R (AAA→AGA) | livM | leucine/isoleucine/valine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/22); total (22/22) |
GATCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAAAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGATGATGCGCTTCGGCGGCCTGC > minE/2632902‑2633037
|
gatCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag > 1:918530/1‑71 (MQ=255)
atCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag > 1:2592053/1‑70 (MQ=255)
atCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag > 1:135491/1‑70 (MQ=255)
atCTGGCGGCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag < 1:3051026/70‑1 (MQ=255)
cTGGCGTCCGCAGGGCTTGCTGCCCATGCCGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag > 1:291827/1‑68 (MQ=255)
cTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag > 1:1095325/1‑68 (MQ=255)
cTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag > 1:2197963/1‑68 (MQ=255)
cTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag > 1:2127507/1‑68 (MQ=255)
ggCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCgagaggagag > 1:2986231/1‑71 (MQ=255)
gCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCgagaggagag < 1:2559182/70‑1 (MQ=255)
gCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCgagaggagag > 1:237023/1‑70 (MQ=255)
gCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCgagaggagag < 1:1065737/70‑1 (MQ=255)
gCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGgaga < 1:2720562/69‑1 (MQ=255)
cGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGc < 1:2817877/70‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:739498/71‑1 (MQ=255)
cAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt > 1:775934/1‑70 (MQ=255)
cAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt < 1:1593365/70‑1 (MQ=255)
tgCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCATCGagag < 1:2218216/54‑1 (MQ=255)
tgcCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCat > 1:273245/1‑71 (MQ=255)
tgcCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCat > 1:2371822/1‑71 (MQ=255)
ccATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCattat > 1:2724080/1‑71 (MQ=255)
cATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGc < 1:552034/50‑1 (MQ=255)
cATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCattatt < 1:1612714/71‑1 (MQ=255)
cATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCattat > 1:2868565/1‑70 (MQ=255)
aTGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCa < 1:804877/50‑1 (MQ=255)
aCGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCt > 1:1346475/1‑71 (MQ=255)
cgcgCCCGGAACTGTAGCTGAAAAAGGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTg < 1:2762355/71‑1 (MQ=255)
cgcCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGtt < 1:907758/71‑1 (MQ=255)
gcCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCa < 1:327419/58‑1 (MQ=255)
cccGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTaa < 1:1045612/71‑1 (MQ=255)
ccGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTaa > 1:30038/1‑70 (MQ=255)
cGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCatt < 1:2753752/57‑1 (MQ=255)
cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc < 1:242833/71‑1 (MQ=255)
cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc > 1:2084243/1‑71 (MQ=255)
cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc < 1:2727950/71‑1 (MQ=255)
aaCTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGcc > 1:2098411/1‑71 (MQ=255)
cTGAAGCTGAAAAACGGCGCAGCGAGAGAAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCtg < 1:1207200/71‑1 (MQ=255)
aaGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCtgat > 1:915026/1‑70 (MQ=255)
cTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGatgatg < 1:1268966/71‑1 (MQ=255)
cTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGatgatg < 1:71159/71‑1 (MQ=255)
tGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGtt < 1:2415506/55‑1 (MQ=255)
gAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGATGATgcg > 1:2807673/1‑71 (MQ=255)
aaaaaCGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCt < 1:1891979/61‑1 (MQ=255)
aaaaCGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATtattat > 1:254684/1‑47 (MQ=255)
aaaaCGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCtgatga > 1:660913/1‑65 (MQ=255)
aaaCGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGATGATGCGCtt > 1:1544114/1‑71 (MQ=255)
aaaCGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGATGATGCGCtt < 1:1131175/71‑1 (MQ=255)
aCGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTaa > 1:1651321/1‑51 (MQ=255)
aCGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGATGATGCGCTTcg < 1:1182019/71‑1 (MQ=255)
gcAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGATGATGCGCTTCggcggc > 1:2442445/1‑71 (MQ=255)
cAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGATGATGCGCTTCGGCGGcc > 1:733075/1‑71 (MQ=255)
cAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGATGATGCGCTTCGGCGGcc > 1:2073276/1‑71 (MQ=255)
gCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACgg < 1:658587/46‑1 (MQ=255)
gCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGATGATGCGCTTCGGCGGCctg > 1:2342183/1‑71 (MQ=255)
cGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGatgatg > 1:685482/1‑55 (MQ=255)
cGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGatgatg > 1:2844866/1‑55 (MQ=255)
cGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGATGATGCGCTTCGGCGGCctgc > 1:2147283/1‑71 (MQ=255)
|
GATCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAAAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGATGATGCGCTTCGGCGGCCTGC > minE/2632902‑2633037
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
