Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F28 I2 R2
|
36 |
87.2 |
4157695 |
97.3% |
4045437 |
63.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
1,314,637 |
A→G |
I298V (ATC→GTC) |
hisB → |
fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,314,637 | 0 | A | G | 100.0%
| 313.0
/ NA
| 84 | I298V (ATC→GTC) | hisB | fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (43/41); total (43/41) |
GTCGCCCGCACCTGGAATATAAAGCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAG > minE/1314569‑1314700
|
gTCGCCCGCACCTGGAATATAAAGCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTc < 1:3955607/71‑1 (MQ=255)
ccGAACCTGGAATATAAAGCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCa < 1:2950627/71‑1 (MQ=255)
gCACCTGGAATATAAAGCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCAct < 1:3571369/71‑1 (MQ=255)
cACCTGGAATATAAAGCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCActt > 1:380408/1‑71 (MQ=255)
ccTGGAATATAAAGCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCActtct < 1:417306/71‑1 (MQ=255)
ggAATATAAAGCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTcc > 1:223860/1‑71 (MQ=255)
gAATATAAAGCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCg < 1:847717/58‑1 (MQ=255)
gAATATAAAGCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACttcttc < 1:2599144/69‑1 (MQ=255)
gAATATAAAGCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACttcttc < 1:2304708/69‑1 (MQ=255)
gAATATAAAGCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCg > 1:955556/1‑71 (MQ=255)
aaTATAAAGCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGt < 1:1254998/71‑1 (MQ=255)
aaTATAAAGCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGt < 1:2040106/71‑1 (MQ=255)
atatAAAGCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGtt > 1:2740167/1‑71 (MQ=255)
atatAAAGCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGtt < 1:2529155/71‑1 (MQ=255)
atatAAAGCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGtt < 1:840998/71‑1 (MQ=255)
atatAAAGCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGtt < 1:1154314/71‑1 (MQ=255)
ataAAGCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTc > 1:1163471/1‑70 (MQ=255)
ataAAGCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCg > 1:872673/1‑71 (MQ=255)
taAATCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGc < 1:1523828/56‑1 (MQ=255)
taAAGCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGc < 1:563481/56‑1 (MQ=255)
aaaGCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGtt > 1:1519724/1‑67 (MQ=255)
aaGCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTcc < 1:3065811/63‑1 (MQ=255)
aaGCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGctc < 1:3793177/71‑1 (MQ=255)
aaGCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGctc < 1:2535519/71‑1 (MQ=255)
aGCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGctct > 1:1887178/1‑71 (MQ=255)
gCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGctc > 1:866166/1‑69 (MQ=255)
ccGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCa > 1:1081001/1‑71 (MQ=255)
ccGAGTTTACCTACCAGAGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCa > 1:3152290/1‑71 (MQ=255)
gAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATa > 1:368484/1‑71 (MQ=255)
gAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATa < 1:761202/71‑1 (MQ=255)
gTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATaca > 1:2054571/1‑71 (MQ=255)
gTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATaca > 1:3122416/1‑71 (MQ=255)
gTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATaca > 1:989875/1‑71 (MQ=255)
ttACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGc > 1:1809283/1‑45 (MQ=255)
tacctaccAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCa > 1:414156/1‑64 (MQ=255)
tacctaccAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCa > 1:1988263/1‑71 (MQ=255)
tacctaccAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCa > 1:2546840/1‑71 (MQ=255)
cctaccAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATg > 1:2212450/1‑71 (MQ=255)
ctaccAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATaca < 1:3789317/65‑1 (MQ=255)
taccAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCATCCGTTCg > 1:569949/1‑54 (MQ=39)
accAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGc > 1:319267/1‑71 (MQ=255)
accAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGc > 1:1237998/1‑71 (MQ=255)
accAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGc > 1:4109506/1‑71 (MQ=255)
accAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGc > 1:3532436/1‑71 (MQ=255)
ccAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGctct > 1:3239844/1‑56 (MQ=255)
ccAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGc < 1:1568361/70‑1 (MQ=255)
cAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGt < 1:1006655/71‑1 (MQ=255)
cAGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGt < 1:519130/71‑1 (MQ=255)
aGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGt > 1:3370652/1‑70 (MQ=255)
aGCGCGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTg < 1:2726949/71‑1 (MQ=255)
gcgcGTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGt < 1:2698460/46‑1 (MQ=255)
gcgTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACg > 1:581528/1‑71 (MQ=255)
gcgTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACg > 1:714002/1‑71 (MQ=255)
gcgTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACg > 1:1028214/1‑71 (MQ=255)
gTGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGc < 1:1823142/70‑1 (MQ=255)
tGGGCGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCt > 1:3479822/1‑70 (MQ=255)
cGATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACAcc < 1:2960270/71‑1 (MQ=255)
gATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTTGCtctc > 1:3225240/1‑44 (MQ=38)
gATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCtctc > 1:1607953/1‑44 (MQ=255)
gATCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCt > 1:3420985/1‑71 (MQ=255)
aTCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGct < 1:2073165/40‑1 (MQ=255)
aTCTCAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTac < 1:140816/66‑1 (MQ=255)
cAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGaaaa > 1:3325364/1‑71 (MQ=255)
cAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGaaaa > 1:1464577/1‑71 (MQ=255)
cAGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGaaaa > 1:2210318/1‑71 (MQ=255)
aGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGaa < 1:1295823/68‑1 (MQ=255)
aGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGa < 1:1357701/67‑1 (MQ=255)
aGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGa < 1:1792023/67‑1 (MQ=255)
aGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAAc > 1:161462/1‑71 (MQ=255)
aGCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAAc > 1:2874393/1‑71 (MQ=255)
gCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGt < 1:989518/52‑1 (MQ=255)
gCACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCt < 1:372597/64‑1 (MQ=255)
cACCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCa < 1:3173532/71‑1 (MQ=255)
aCCGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATg < 1:1875064/45‑1 (MQ=255)
ccGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGaa < 1:3014062/64‑1 (MQ=255)
ccGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGaa < 1:394915/64‑1 (MQ=255)
ccGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCaaa < 1:1993106/71‑1 (MQ=255)
ccGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCaaa < 1:2870837/71‑1 (MQ=255)
ccGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCaa < 1:2502780/70‑1 (MQ=255)
ccGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCa > 1:868059/1‑69 (MQ=255)
ccGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCa > 1:1396197/1‑69 (MQ=255)
ccGAAATGGTCGAGCACTTCTTCCGTTCGATCTCATACACCATGGGCGTGACGCTACACCTGAAAACCaaa < 1:3825410/71‑1 (MQ=255)
cGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAg > 1:2942698/1‑71 (MQ=255)
cGAAATGGTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAg > 1:219451/1‑71 (MQ=255)
|
GTCGCCCGCACCTGGAATATAAAGCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAG > minE/1314569‑1314700
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
