breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 74,556 G→A S61S (TCG→TCA mraZ → conserved hypothetical protein
RA 128,514 G→A V25M (GTG→ATG)  yadH → predicted transporter subunit
RA 131,828 C→T L41F (CTC→TTC)  yadD → predicted transposase
RA 171,024 C→T D115D (GAC→GAT pyrH → uridylate kinase
RA 204,511 C→T intergenic (+111/‑73) alaV → / → rrlH tRNA‑Ala/23S ribosomal RNA
RA 204,556 A→G intergenic (+156/‑28) alaV → / → rrlH tRNA‑Ala/23S ribosomal RNA
RA 269,873 G→A intergenic (‑61/‑145) xseB ← / → thiI exonuclease VII small subunit/sulfurtransferase required for thiamine and 4‑thiouridine biosynthesis
RA 364,085 C→T G15D (GGC→GAC)  intD ← predicted integrase
RA 442,228 C→A A512S (GCG→TCG)  speF ← ornithine decarboxylase isozyme, inducible
RA 474,288 T→C V617A (GTT→GCT)  mngA → fused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzyme IIABC components
RA 667,574 C→A T304T (ACC→ACA uup → fused predicted transporter subunits and ATP‑binding components of ABC superfamily
RA 760,045 T→C T160A (ACG→GCG)  ycfZ ← predicted inner membrane protein
RA 786,414 G→A T173I (ACC→ATC)  nhaB ← sodium:proton antiporter
RA 857,377 T→C intergenic (‑8/+4) trpB ← / ← trpC tryptophan synthase, beta subunit/fused indole‑3‑glycerolphosphate synthetase and N‑(5‑phosphoribosyl)anthranilate isomerase
RA 1,036,505 G→A A632A (GCG→GCA rsxC → fused predicted 4Fe‑4S ferredoxin‑type protein
RA 1,312,925 G→A A84T (GCG→ACG)  hisC → histidinol‑phosphate aminotransferase
RA 1,314,637 A→G I298V (ATC→GTC)  hisB → fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase
RA 1,348,802 C→A A473S (GCG→TCG)  asmA ← predicted assembly protein
RA 1,461,819 T→C pseudogene (136/4605 nt)
pseudogene (136/4104 nt)
yfaS ←
yfaS ←
ECK2220:JW2222+JW2221:b4500; hypothetical protein
ECK2220:JW2222:b2228; hypothetical protein, N‑ter fragment
RA 1,695,569 C→T M147I (ATG→ATA yfhQ ← predicted methyltransferase
RA 1,771,424 C→T P332S (CCG→TCG)  pheA → fused chorismate mutase P and prephenate dehydratase
RA 1,869,315 T→C K147K (AAA→AAG ygcW ← predicted deoxygluconate dehydrogenase
RA 1,892,019 +GC intergenic (‑437/+54) gudP ← / ← ygdL predicted D‑glucarate transporter/conserved hypothetical protein
RA 2,143,361 T→C noncoding (22/77 nt) metY ← tRNA‑Met
RA 2,223,251 T→C R121R (CGT→CGC panF → pantothenate:sodium symporter
RA 2,349,606 A→G V681A (GTG→GCG)  polA ← fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease
RA 2,364,370 T→C N367D (AAC→GAC)  trkH ← potassium transporter
RA 2,370,014 G→A V58V (GTG→GTA fadA → 3‑ketoacyl‑CoA thiolase
RA 2,415,011 A→G L215L (CTT→CTC aslB ← predicted regulator of arylsulfatase activity
RA 2,478,588 C→T I187I (ATC→ATT pstB → phosphate transporter subunit
RA 2,509,289 T→C intergenic (+34/+19) yicI → / ← yicH predicted alpha‑glucosidase/conserved hypothetical protein
RA 2,674,677 G→A intergenic (‑140/+220) gntT ← / ← gntY gluconate transporter, high‑affinity GNT I system/predicted gluconate transport associated protein
RA 2,697,811 G→A R139C (CGT→TGT)  mrcA ← fused penicillin‑binding protein 1a murein transglycosylase and murein transpeptidase
RA 2,706,320 C→T A95V (GCC→GTC)  rpe → D‑ribulose‑5‑phosphate 3‑epimerase
RA 2,721,165 A→G L139S (TTG→TCG)  yhfK ← conserved inner membrane protein
RA 2,974,149 A→C D182A (GAT→GCT)  nadR → bifunctional DNA‑binding transcriptional repressor and NMN adenylyltransferase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ minE 251992 252035 44 21 [19] [19] 20 malZ maltodextrin glucosidase
* * ÷ minE 1420582 1420630 49 20 [19] [19] 21 ccmF heme lyase, CcmF subunit