Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F28 I2 R2
|
36 |
87.2 |
4157695 |
97.3% |
4045437 |
63.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
2,415,011 |
A→G |
L215L (CTT→CTC) |
aslB ← |
predicted regulator of arylsulfatase activity |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,415,011 | 0 | A | G | 98.2%
| 206.9
/ NA
| 56 | L215L (CTT→CTC) | aslB | predicted regulator of arylsulfatase activity |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (20/35); minor base T (0/1); total (20/36) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGAACTCCTCGGGGCGCACCGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTAAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGACCACCGGAATAAACTGGATGAATTC > minE/2414944‑2415076
|
cGAACTCCTCGGGGCGCACCGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGt > 1:1934181/1‑71 (MQ=255)
aaCTCCTCGGGGCGCACCGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTcc < 1:3512572/71‑1 (MQ=255)
aCTCCTCGGGGCGCACCGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCa < 1:109030/71‑1 (MQ=255)
aCTCCTCGGGGCGCACCGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCa < 1:337113/71‑1 (MQ=255)
tcGGGGCGCACCGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTcc < 1:320284/65‑1 (MQ=255)
cGGGGCGCACCGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAg > 1:1175205/1‑61 (MQ=255)
cGGGGCGCACCGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGAATGTAACTTGAGTCCATCGCgg > 1:2319976/1‑71 (MQ=255)
gcgcACCGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCa < 1:1976246/61‑1 (MQ=255)
gcACCGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAgtt < 1:2905159/71‑1 (MQ=255)
cACCGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAgttg < 1:957465/71‑1 (MQ=255)
ccGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAg > 1:981466/1‑52 (MQ=255)
ccGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAg > 1:1195578/1‑52 (MQ=255)
ccGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAg > 1:123291/1‑52 (MQ=255)
ccGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGt < 1:1312792/53‑1 (MQ=255)
ccGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAgttgtt < 1:1555977/71‑1 (MQ=255)
cGACCATTCCGTTATCTCACCCTGAATATCACCAGGCGCATGTAACTTGAg < 1:1773958/51‑1 (MQ=255)
cGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGttt > 1:1184129/1‑71 (MQ=255)
gACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGttt > 1:2912212/1‑70 (MQ=255)
aCCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCggg > 1:258618/1‑60 (MQ=255)
aCCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTtca < 1:3549946/71‑1 (MQ=255)
ccATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTtcat < 1:1805925/71‑1 (MQ=255)
ttCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAg > 1:2679982/1‑71 (MQ=255)
cGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTtca < 1:3182468/64‑1 (MQ=255)
cGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGcc < 1:2150174/70‑1 (MQ=255)
cGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGcc < 1:913180/70‑1 (MQ=255)
cGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGcc < 1:855948/70‑1 (MQ=255)
cGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCa < 1:2444435/71‑1 (MQ=255)
gTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCTTCAGCCAg < 1:917803/71‑1 (MQ=255)
ttAGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAgg < 1:2890889/71‑1 (MQ=255)
aGCTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGgc < 1:3029048/70‑1 (MQ=255)
cTCACCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAgttgt < 1:1578039/54‑1 (MQ=255)
aCCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTc < 1:917672/69‑1 (MQ=255)
aCCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTc < 1:2105408/69‑1 (MQ=255)
aCCCTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTc < 1:4140029/69‑1 (MQ=255)
cccTGAATATCACCAGGCGCATGTAACTTGAGTcc < 1:4213/35‑1 (MQ=255)
cccTGAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGac > 1:3452223/1‑71 (MQ=255)
gAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGcc > 1:132425/1‑56 (MQ=255)
gAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGaccacc > 1:1688366/1‑71 (MQ=255)
gAATATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGa > 1:1457153/1‑66 (MQ=255)
aaTATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGaccac < 1:1304318/69‑1 (MQ=255)
aaTATCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGCCCACCg > 1:434445/1‑71 (MQ=255)
atatCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTTTTTCATCAGCCAGGCGCTCGACCACCgg < 1:2591342/71‑1 (MQ=255)
tCACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGACCACCGGaa > 1:2252280/1‑70 (MQ=255)
cACCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGACCACCGGAAt > 1:3314602/1‑70 (MQ=255)
aCCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGACCACCGGAATaa < 1:3054316/71‑1 (MQ=255)
aCCAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGACCACCGGAATa > 1:4127104/1‑70 (MQ=255)
ccAGGCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGac > 1:3843034/1‑59 (MQ=255)
ggCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTtcat > 1:1540493/1‑40 (MQ=255)
ggCGCATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGACCACCGGAATAAACTg < 1:1044763/71‑1 (MQ=255)
gcgcATGTAACTTTAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGgcg < 1:2129018/49‑1 (MQ=255)
gcgcATGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGACCACCGGAATAAACTgg < 1:3471846/71‑1 (MQ=255)
aTGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGACCACCGGAATAAACTGGATGa < 1:833568/71‑1 (MQ=255)
tGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGc < 1:3390977/38‑1 (MQ=255)
tGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGACCACCGGAATAAACTGGATGaa < 1:779468/71‑1 (MQ=255)
tGTAACTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGACCACCGGAATAAACTGGATGaa < 1:3249919/71‑1 (MQ=255)
aCTTGAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGACCACCGGAATAAACTGGATGAATTc < 1:1170250/70‑1 (MQ=255)
|
CGAACTCCTCGGGGCGCACCGACCATTCCGTTAGCTCACCCTGAATATCACCAGGCGCATGTAACTTAAGTCCATCGCGGGCAGTTGTTTCATCAGCCAGGCGCTCGACCACCGGAATAAACTGGATGAATTC > minE/2414944‑2415076
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
