Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I2 R2 36 87.2 4157695 97.3% 4045437 63.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 2,706,320 C→T A95V (GCC→GTC)  rpe → D‑ribulose‑5‑phosphate 3‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,706,3200CT99.2% 349.9 / NA 117A95V (GCC→GTC) rpeD‑ribulose‑5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (50/66);  minor base G (0/1);  total (50/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

ACCCGTCGATCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGCCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTAAAGCGGGTCTGGTATT  >  minE/2706252‑2706386
                                                                    |                                                                  
aCCCGTCGATCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCt                                                                  <  1:2573391/71‑1 (MQ=255)
 cccGTCGATCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGtctc                                                                 <  1:3157026/71‑1 (MQ=255)
  ccGTCGATCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTcc                                                                <  1:293850/71‑1 (MQ=255)
    gTCGATCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTcc                                                                >  1:1702599/1‑69 (MQ=255)
    gTCGATCGCATTGTGCCTGATTTCGCTG‑CGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAg                                                             <  1:1609322/71‑1 (MQ=255)
     tCGATCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAg                                                             >  1:3585209/1‑71 (MQ=255)
     tCGATCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAg                                                             >  1:1616176/1‑71 (MQ=255)
      cGATCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAg                                                             >  1:1092328/1‑70 (MQ=255)
       gATCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGc                                                            <  1:2955245/70‑1 (MQ=255)
       gATCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGc                                                            <  1:1797386/70‑1 (MQ=255)
       gATCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCa                                                           >  1:2179924/1‑71 (MQ=255)
       gATCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCa                                                           <  1:3879219/71‑1 (MQ=255)
       gATCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCa                                                           <  1:2332568/71‑1 (MQ=255)
       gATCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCa                                                           <  1:2811872/71‑1 (MQ=255)
         tCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATg                                                         <  1:2947571/71‑1 (MQ=255)
         tCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATg                                                         >  1:904003/1‑71 (MQ=255)
          cGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGt                                                        >  1:1442230/1‑71 (MQ=255)
          cGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGt                                                        >  1:2036810/1‑71 (MQ=255)
          cGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGt                                                        >  1:3604225/1‑71 (MQ=255)
           gCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGtt                                                       <  1:3253389/71‑1 (MQ=255)
           gCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGtt                                                       <  1:2327132/71‑1 (MQ=255)
            cATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTg                                                      <  1:2345668/71‑1 (MQ=255)
             aTTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGa                                                     <  1:1363530/71‑1 (MQ=255)
             aTTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGa                                                     <  1:3013219/71‑1 (MQ=255)
              ttGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGAc                                                    <  1:3969923/71‑1 (MQ=255)
                   ccTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGAcc                                                   >  1:699826/1‑67 (MQ=255)
                   ccTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGAcc                                                   >  1:1398226/1‑67 (MQ=255)
                   ccTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCAAGAAGTCTCCGAGCATGTTGa                                                     >  1:3621569/1‑65 (MQ=255)
                    cTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGAGCGCACg                                              <  1:5038/71‑1 (MQ=255)
                    cTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACg                                              >  1:1966094/1‑71 (MQ=255)
                    cTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACg                                              <  1:456489/71‑1 (MQ=255)
                     tGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGc                                             >  1:2694495/1‑71 (MQ=255)
                      gATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCa                                                           >  1:1116330/1‑56 (MQ=255)
                      gATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCa                                                           >  1:3062318/1‑56 (MQ=255)
                      gATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCa                                                           >  1:2765796/1‑56 (MQ=255)
                      gATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCt                                            >  1:3385460/1‑71 (MQ=255)
                        tttCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGc                                          <  1:1899882/71‑1 (MQ=255)
                            gCTGCCGCTGGTGCCAGCATCATTAGCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACt                                      <  1:3007508/71‑1 (MQ=255)
                            gCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAAc                                       <  1:891350/70‑1 (MQ=255)
                            gCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACt                                      <  1:1359238/71‑1 (MQ=255)
                             cTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCTCGCTGCAACTg                                     <  1:132997/71‑1 (MQ=255)
                             cTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAAc                                       <  1:507371/69‑1 (MQ=255)
                             cTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAAc                                       <  1:696539/69‑1 (MQ=255)
                             cTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAAc                                       <  1:3775602/69‑1 (MQ=255)
                             cTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTg                                     <  1:833729/71‑1 (MQ=255)
                              tGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCa                                                <  1:205673/59‑1 (MQ=255)
                                ccGCTGGTTCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGa                                    <  1:2638785/69‑1 (MQ=255)
                                ccGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCa                                                >  1:956119/1‑57 (MQ=255)
                                ccGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCa                                                >  1:1321499/1‑57 (MQ=255)
                                 cGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTcc                                                                >  1:3758207/1‑40 (MQ=255)
                                 cGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTa                                 <  1:2872428/71‑1 (MQ=255)
                                 cGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTa                                 <  1:1790926/71‑1 (MQ=255)
                                  gCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACt                                      <  1:3419576/65‑1 (MQ=255)
                                  gCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTa                                 <  1:2683238/70‑1 (MQ=255)
                                  gCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTa                                 >  1:4141091/1‑70 (MQ=255)
                                  gCTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTa                                 <  1:3181666/70‑1 (MQ=255)
                                   cTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCa                                                           <  1:1145478/43‑1 (MQ=255)
                                   cTGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGa                                                     >  1:2070338/1‑49 (MQ=255)
                                    tGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATg                                                         >  1:1115635/1‑44 (MQ=255)
                                    tGGTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTa                                 >  1:1780762/1‑68 (MQ=255)
                                     ggTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGtt                                                       >  1:3120597/1‑45 (MQ=255)
                                     ggTGCCAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGtt                                                       >  1:1423751/1‑45 (MQ=255)
                                         ccAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGaaaa                          <  1:1211020/70‑1 (MQ=255)
                                         ccAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGaaaa                          <  1:2242210/70‑1 (MQ=255)
                                         ccAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAAt                         <  1:1178372/71‑1 (MQ=255)
                                         ccAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAAt                         <  1:4136296/71‑1 (MQ=255)
                                          cAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACt                                      <  1:2155805/57‑1 (MQ=255)
                                          cAGCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACt                                      <  1:3394431/57‑1 (MQ=255)
                                           aGCATCATTACCTTTCATCCAGAAGTCTACGAGCATGTTGACCGCACGCTGCAACTGAt                                   >  1:3499379/1‑59 (MQ=255)
                                            gCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGc                      >  1:1933762/1‑71 (MQ=255)
                                            gCATCATTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGc                      >  1:3855494/1‑71 (MQ=255)
                                               tcatTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGc                                             <  1:1255717/45‑1 (MQ=255)
                                               tcatTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGc                                             <  1:1562032/45‑1 (MQ=255)
                                               tcatTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTg                    <  1:184378/70‑1 (MQ=255)
                                                 atTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTa                  <  1:3257831/70‑1 (MQ=255)
                                                 atTACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTa                  <  1:1457888/70‑1 (MQ=255)
                                                 atTACCTTTCATCCAGAAGGCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTa                  <  1:3683716/70‑1 (MQ=255)
                                                  ttACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGa                             >  1:3240643/1‑58 (MQ=255)
                                                  ttACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTa                  >  1:771996/1‑69 (MQ=255)
                                                  ttACCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTa                  >  1:2402526/1‑69 (MQ=255)
                                                    aCCTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTAAAg               <  1:3907479/70‑1 (MQ=255)
                                                     ccTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTAAAGc              <  1:328266/70‑1 (MQ=255)
                                                      cTTTCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGAt                                   <  1:3840944/48‑1 (MQ=255)
                                                       tttCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAg                              <  1:440624/52‑1 (MQ=255)
                                                        ttCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGa                                    >  1:3539895/1‑45 (MQ=255)
                                                        ttCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTa                                 >  1:1259612/1‑48 (MQ=255)
                                                        ttCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGaaaa                          <  1:3543855/55‑1 (MQ=255)
                                                         tCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTAAAGCggg           <  1:3372425/69‑1 (MQ=255)
                                                         tCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTAAAGCggg           <  1:3329170/69‑1 (MQ=255)
                                                         tCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTAAAGCggg           <  1:315463/69‑1 (MQ=255)
                                                         tCATCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTAAAGCGGGTc         >  1:2059963/1‑71 (MQ=255)
                                                           aTCCAGAAGTCTCCGAGCATGTTGACCGCACGTTGCAACTGa                                    >  1:3850983/1‑42 (MQ=38)
                                                           aTCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTAAAGCGGGTCt        <  1:2003460/70‑1 (MQ=255)
                                                           aTCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTAAAGCGGGTCTg       <  1:1528830/71‑1 (MQ=255)
                                                            tCCAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTAAAGCGGGTCTgg      <  1:1605515/71‑1 (MQ=255)
                                                              cAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATgg                       >  1:2322127/1‑52 (MQ=255)
                                                              cAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATgg                       >  1:1450395/1‑52 (MQ=255)
                                                              cAGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTAAAGCGGGTCTGGTa    <  1:2897187/71‑1 (MQ=255)
                                                               aGAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTAAAGCGGGTCTGGTAt   >  1:2131948/1‑71 (MQ=255)
                                                                gAAGTCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTAAAGCGGGTCTGGTAtt  >  1:153349/1‑71 (MQ=255)
                                                                    |                                                                  
ACCCGTCGATCGCATTGTGCCTGATTTCGCTGCCGCTGGTGCCAGCATCATTACCTTTCATCCAGAAGCCTCCGAGCATGTTGACCGCACGCTGCAACTGATTAAAGAAAATGGCTGTAAAGCGGGTCTGGTATT  >  minE/2706252‑2706386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A