Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F17 I0 R1	71	95.7	2922988	91.7%	2680379	65.6

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	236,898	G→A	100%	E85K (GAA→AAA)	yaiE →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	236,898	0	G	A	89.2%	105.3 / 5.0	37	E85K (GAA→AAA)	yaiE	conserved hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (2/2); new base A (33/0); total (35/2)
Fisher's exact test for biased strand distribution p-value = 9.01e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGTGTTTAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCTT > minE/236852‑236941

ggTGTTTAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGc                      <  1:2431988/70‑1 (MQ=255)

ggTGTTTAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGc                      <  1:46158/70‑1 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCaaa                                           >  1:1521581/1‑43 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCt                 >  1:923052/1‑69 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCTg                >  1:2897444/1‑70 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCTg                >  1:1237287/1‑70 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCTg                >  1:295695/1‑70 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCTg                >  1:313074/1‑70 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCTg                >  1:314568/1‑70 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCTg                >  1:345244/1‑70 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCTg                >  1:399028/1‑70 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCTg                >  1:435754/1‑70 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCTg                >  1:530520/1‑70 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCTg                >  1:567504/1‑70 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCTg                >  1:653308/1‑70 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCTg                >  1:675111/1‑70 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCTg                >  1:67845/1‑70 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCTg                >  1:706140/1‑70 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCTg                >  1:706737/1‑70 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCTg                >  1:81662/1‑70 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCTg                >  1:2788383/1‑70 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCTg                >  1:1399026/1‑70 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCTg                >  1:15043/1‑70 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCTg                >  1:1822912/1‑70 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCTg                >  1:1973775/1‑70 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCTg                >  1:2044611/1‑70 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCTg                >  1:2098899/1‑70 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCTg                >  1:2207000/1‑70 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCTg                >  1:2268706/1‑70 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCTg                >  1:2270036/1‑70 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCTg                >  1:2424517/1‑70 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCTg                >  1:2526532/1‑70 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCTg                >  1:2544766/1‑70 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCTg                >  1:2605287/1‑70 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGCTATCTg                >  1:2877350/1‑70 (MQ=255)

      tAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCAAACCCACCTCTTATCTGTGCCGATATCTg                >  1:961046/1‑70 (MQ=255)

                   cacaGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCtt  >  1:2095987/1‑71 (MQ=255)

                   cacaGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCtt  >  1:1232117/1‑71 (MQ=255)

GGTGTTTAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCTT > minE/236852‑236941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

N/A