Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F17 I0 R1
|
71 |
95.7 |
2922988 |
91.7% |
2680379 |
65.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
484,358 |
G→A |
100% |
G55D (GGC→GAC) |
tolB → |
periplasmic protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 484,358 | 0 | G | A | 90.6%
| 92.8
/ 2.1
| 32 | G55D (GGC→GAC) | tolB | periplasmic protein |
| Reads supporting (aligned to +/- strand): ref base G (3/0); new base A (29/0); total (32/0) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01 |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GTTCCTTTCCAGTGGGCGGGGCCTGGTGCGGCACCTGAAGATATTGGCGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCCACA > minE/484312‑484427
|
gttCCTTTCCAGTGGGCTGGGCCTGGTGCGGCACCTGAAGATATTGGCGGCATCGTTGCTGCTGACTTGCg > 1:274294/1‑71 (MQ=255)
gttCCTTTCCAGTGGGCGGGGCCTGGTGCGGCACCTGAAGATATTGGCGGCATCGTTGCTGCTGACTTGCg > 1:2886427/1‑71 (MQ=255)
gttCCTTTCCAGTGGGCGGGGCCTGGTGCGGCACCTGAAGATATTGGCGGCATCGTTGCTGCTGACTTGCg > 1:855958/1‑71 (MQ=255)
ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGc > 1:2138843/1‑64 (MQ=255)
ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTaaa > 1:296101/1‑70 (MQ=255)
ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:1647418/1‑71 (MQ=255)
ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:2255289/1‑71 (MQ=255)
ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:1694964/1‑71 (MQ=255)
ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:1793604/1‑71 (MQ=255)
ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:50741/1‑71 (MQ=255)
ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:187200/1‑71 (MQ=255)
ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:1894970/1‑71 (MQ=255)
ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:2365508/1‑71 (MQ=255)
ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:404046/1‑71 (MQ=255)
ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:395395/1‑71 (MQ=255)
ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:367855/1‑71 (MQ=255)
ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:2142325/1‑71 (MQ=255)
ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:2153626/1‑71 (MQ=255)
ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:2195419/1‑71 (MQ=255)
ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:1616369/1‑71 (MQ=255)
ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:1044793/1‑71 (MQ=255)
ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:1462820/1‑71 (MQ=255)
ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:609183/1‑71 (MQ=255)
ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:1428636/1‑71 (MQ=255)
ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:1375557/1‑71 (MQ=255)
ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:244365/1‑71 (MQ=255)
ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:522404/1‑71 (MQ=255)
ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:1274778/1‑71 (MQ=255)
ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:893081/1‑71 (MQ=255)
ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:1190839/1‑71 (MQ=255)
ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:2557745/1‑71 (MQ=255)
ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:2695901/1‑71 (MQ=255)
ggcggcATGGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:2445603/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGcc > 1:2916466/1‑68 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGc > 1:407856/1‑67 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCcacg > 1:2772258/1‑70 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCcac > 1:391074/1‑70 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:412042/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:603397/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:663126/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:680861/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:342665/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:725430/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:847798/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:89564/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:917964/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:2798539/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:278859/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:93342/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:968337/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:238167/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:1628932/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:1134384/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:1139744/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:1166572/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:1229049/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:1230393/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:12571/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:1261433/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:1293609/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:1309864/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:1368543/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:1452069/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:1536329/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:2611000/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:1797397/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:1923668/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:2032605/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:2075731/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:2087631/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:2134358/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:2312437/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:2459043/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:251012/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:251120/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:2543120/1‑69 (MQ=255)
ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:260872/1‑69 (MQ=255)
|
GTTCCTTTCCAGTGGGCGGGGCCTGGTGCGGCACCTGAAGATATTGGCGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCCACA > minE/484312‑484427
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
