Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F8 I0 R1
|
311 |
50.1 |
4085234 |
95.9% |
3917739 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
342,943 |
T→C |
47.1% |
V279A (GTT→GCT) |
yahK → |
predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 342,943 | 0 | T | C | 47.1%
| ‑0.9
/ 46.8
| 34 | V279A (GTT→GCT) | yahK | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base T (5/13); new base C (6/10); total (11/23) |
| Fisher's exact test for biased strand distribution p-value = 7.17e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTGGCGGCATTCCAGAAACTCAGGA > W3110S.gb/342876‑343012
|
ttGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGctt > 1:2196283/1‑70 (MQ=255)
ttGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGctt > 1:2015202/1‑70 (MQ=255)
tGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGtttt > 1:3217427/1‑70 (MQ=255)
tGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGtttt > 1:926387/1‑70 (MQ=255)
tGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGcttt > 1:1279413/1‑70 (MQ=255)
gCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTc > 1:2880696/1‑70 (MQ=255)
gTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGtttt > 1:3329509/1‑61 (MQ=255)
tGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGCTTTCAACCTGATCa > 1:931665/1‑71 (MQ=255)
tGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGa < 1:1824918/71‑1 (MQ=255)
tGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGa < 1:3559446/71‑1 (MQ=255)
tGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGCTTTCAACCTGATCATGa < 1:2761493/71‑1 (MQ=255)
ccATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGa < 1:2255063/66‑1 (MQ=255)
ccATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGa < 1:2167708/66‑1 (MQ=255)
tGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGtt < 1:2011325/47‑1 (MQ=255)
tGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCt < 1:3798409/55‑1 (MQ=255)
aCGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGc < 1:3535047/71‑1 (MQ=255)
aCGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGCTTTCAACCTGATCATGAAACGCCGTGc < 1:1596688/71‑1 (MQ=255)
cGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCg < 1:9264/71‑1 (MQ=255)
gCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGCTTTCAACCTGATCATGAAACGCCGTGCGa < 1:1630167/71‑1 (MQ=255)
gCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGCTTTCAACCTGATCATGAAACGCCGTGCGa < 1:3974055/71‑1 (MQ=255)
cTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTTATCATg < 1:714/44‑1 (MQ=255)
cTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCa > 1:375164/1‑42 (MQ=255)
cTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCa > 1:2728287/1‑42 (MQ=255)
cTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATg < 1:1578829/44‑1 (MQ=255)
cTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATg < 1:1005612/44‑1 (MQ=255)
cTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATg < 1:1098674/71‑1 (MQ=255)
tGCGACACCGCATAAATCGCCGGAAGCTTTCAACCTGATCATg < 1:1512923/43‑1 (MQ=255)
tGCGACACCGCATAAATCGCCGGAAGCTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGa > 1:246131/1‑71 (MQ=255)
acCGCATAAATCGCCGGAAGTTTTAAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGAt > 1:2026514/1‑66 (MQ=255)
cGCATAAATCGCCGGAAGCTTTCAACCTGATCATGAAACGCCGTGCGa > 1:2663557/1‑48 (MQ=255)
cGCATAAATCGCCGGAAGCTTTCAACCTGATCATGAAACGCCGTGCGa > 1:3909504/1‑48 (MQ=255)
cGCATAAATCGCCGGAAGCTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTggcggc < 1:1252525/71‑1 (MQ=255)
gCATAAATCGCCGGAAGCTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTGGCGGCa > 1:3462437/1‑71 (MQ=255)
gCATAAATCGCCGGAAGCTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTGGCGGCa > 1:1873640/1‑71 (MQ=255)
tAAATCGCCGGAAGCTTTCAACCTGATCATGAAACGCCGTGc < 1:2474617/42‑1 (MQ=255)
aaTCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTGGCGGCATTc > 1:1152301/1‑69 (MQ=255)
tCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTGGCGGCATTCCAGa < 1:3078977/71‑1 (MQ=255)
tCGCCGGAAGCTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTc < 1:1815170/50‑1 (MQ=255)
tCGCCGGAAGCTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTc < 1:107141/50‑1 (MQ=255)
gCCGGAAGCTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTGGCGGCATTCCAGaa < 1:19339/70‑1 (MQ=255)
gTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTGGCGGCATTCCAGAAACTCAGga < 1:697246/71‑1 (MQ=255)
ttttCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTGGCGGCATTCCAGAAACTc < 1:2294193/66‑1 (MQ=255)
ttttCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTGGCGGCATTCCAGAAACTc < 1:3686626/66‑1 (MQ=255)
|
TTGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTGGCGGCATTCCAGAAACTCAGGA > W3110S.gb/342876‑343012
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
